Drug targeting : strategies, principles, and applications /
Drug targeting : strategies, principles, and applications /
edited by G.E. Francis and Cristina Delgado.
- Totowa, N.J. : Humana Press, ©2000.
- 1 online resource (x, 318 pages) : illustrations
- Methods in molecular medicine ; 25 .
- Methods in molecular medicine ; 25. .
Includes bibliographical references and index.
pt. 1. Strategies -- Chemical construction of immunotoxins -- Construction of ribonuclease-antibody conjugates for selective cytotoxicity -- Intracellular targeting using bispecific antibodies -- Antibody conjugation methods for active targeting of liposomes -- Folate as a targeting device for proteins utilizing folate receptor-mediated endocytosis -- Preparation of recombinant RNase single-chain antibody fusion proteins -- pt. 2. Theoretical aspects -- Practical considerations in the exploitation of passive tumor targeting -- Effect of dose, molecular size, and binding affinity on uptake of antibodies -- Inhibition of tumor blood flow -- pt. 3. Applications -- Targeting in myocardial infarction -- Targeting HIV-infected cells -- Making fusion toxins to targetleukemia and lymphoma -- Antibody-bearing liposomes as chloroquine vehicles in treatment of murine malaria -- Immunotargeting of catalase to the pulmonary vascular endothelium -- Targeted gene transfer: a practical guide based on experience with lipid-based plasmid delivery systems.
Annotation G.E. Francis and Cristina Delgado have assembled a comprehensive collection of leading-edge protocols for drug targeting studies. In addition to the detailed step-by-step instruction, the protocols review the principal current targeting strategies, discuss how to build the constructs, examine theoretical considerations relevant to solid tumors, and describe experimental and clinical applications of targeted drug delivery systems. Also present are new chemical and biological approaches to the preparation of targeted biopharmaceuticals, including ribonuclease-antibody chimeras, bispecific antibodies, enzyme-antibody conjugates, and folate-bearing conjugates. Applications include the targeting of myocardial infarctions using liposomal systems, the use of monoclonal antibodies to target malaria and HIV-infected cells, the targeting of fusion proteins to leukemias and lymphomas, and the use of antioxidant enzyme-antibody conjugates to target the pulmonary endothelium. Comprehensive and innovative, Drug Targeting: Strategies, Principles, and Applications documents in practical detail the major new chemical and biological targeting methods now being used to produce today's emerging rich array of novel diagnostic agents and promising biopharmaceuticals.
English.
0585395098 9780585395098 1592590756 9781592590759 9786610820658 6610820651 1280820659 9781280820656
10.1385/1592590756. doi
089603531X 00012511
Drug delivery systems.
Drug carriers (Pharmacy)
Drug Delivery Systems
Drug Carriers
Pharmaceutical Vehicles
Systèmes d'administration de médicaments.
Vecteurs de médicaments.
MEDICAL--Pharmacology.
Drug carriers (Pharmacy)
Drug delivery systems
Laboratory Manual
Internet Resources.
Laboratory Manuals.
Laboratory manuals.
Manuels de laboratoire.
RM301.63 / .D785 2000
615/.7
2000 I-485 WB 330 / D7973 2000
Includes bibliographical references and index.
pt. 1. Strategies -- Chemical construction of immunotoxins -- Construction of ribonuclease-antibody conjugates for selective cytotoxicity -- Intracellular targeting using bispecific antibodies -- Antibody conjugation methods for active targeting of liposomes -- Folate as a targeting device for proteins utilizing folate receptor-mediated endocytosis -- Preparation of recombinant RNase single-chain antibody fusion proteins -- pt. 2. Theoretical aspects -- Practical considerations in the exploitation of passive tumor targeting -- Effect of dose, molecular size, and binding affinity on uptake of antibodies -- Inhibition of tumor blood flow -- pt. 3. Applications -- Targeting in myocardial infarction -- Targeting HIV-infected cells -- Making fusion toxins to targetleukemia and lymphoma -- Antibody-bearing liposomes as chloroquine vehicles in treatment of murine malaria -- Immunotargeting of catalase to the pulmonary vascular endothelium -- Targeted gene transfer: a practical guide based on experience with lipid-based plasmid delivery systems.
Annotation G.E. Francis and Cristina Delgado have assembled a comprehensive collection of leading-edge protocols for drug targeting studies. In addition to the detailed step-by-step instruction, the protocols review the principal current targeting strategies, discuss how to build the constructs, examine theoretical considerations relevant to solid tumors, and describe experimental and clinical applications of targeted drug delivery systems. Also present are new chemical and biological approaches to the preparation of targeted biopharmaceuticals, including ribonuclease-antibody chimeras, bispecific antibodies, enzyme-antibody conjugates, and folate-bearing conjugates. Applications include the targeting of myocardial infarctions using liposomal systems, the use of monoclonal antibodies to target malaria and HIV-infected cells, the targeting of fusion proteins to leukemias and lymphomas, and the use of antioxidant enzyme-antibody conjugates to target the pulmonary endothelium. Comprehensive and innovative, Drug Targeting: Strategies, Principles, and Applications documents in practical detail the major new chemical and biological targeting methods now being used to produce today's emerging rich array of novel diagnostic agents and promising biopharmaceuticals.
English.
0585395098 9780585395098 1592590756 9781592590759 9786610820658 6610820651 1280820659 9781280820656
10.1385/1592590756. doi
089603531X 00012511
Drug delivery systems.
Drug carriers (Pharmacy)
Drug Delivery Systems
Drug Carriers
Pharmaceutical Vehicles
Systèmes d'administration de médicaments.
Vecteurs de médicaments.
MEDICAL--Pharmacology.
Drug carriers (Pharmacy)
Drug delivery systems
Laboratory Manual
Internet Resources.
Laboratory Manuals.
Laboratory manuals.
Manuels de laboratoire.
RM301.63 / .D785 2000
615/.7
2000 I-485 WB 330 / D7973 2000