Single domain antibodies : methods and protocols /
Single domain antibodies : methods and protocols /
edited by Dirk Saerens and Serge Muyldermans.
- New York : Humana Press, ©2012.
- 1 online resource (xv, 580 pages) : illustrations (some color)
- Methods in molecular biology, 911 1940-6029 ; .
- Methods in molecular biology (Clifton, N.J.) ; 911. .
Includes bibliographical references and index.
From whole monoclonal antibodies to single domain antibodies : think small / Introduction to heavy chain antibodies and derived nanobodies / Overview and discovery of IgNARs and generation of VNARs / Creation of the large and highly functional synthetic repertoire of human VH and Vk domain antibodies / Preparation of a naive library of camelid single domain antibodies / Selection by phage display of single domain antibodies specific to antigens in their native conformation / Semiautomated panning of naive camelidae libraries and selection of single-domain antibodies against peptide antigens / Pichia surface display : a tool for screening single domain antibodies / Bacterial two hybrid : a versatile one-step intracellular selection method / Intracellular antibody capture (IAC) methods for single domain antibodies / Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells : a 'one plasmid' mammalian two-hybrid system / Cell-free selection of domain antibodies by in vitro compartmentalization / Selection of VHHs under application conditions / Isolation and characterization of Clostridium difficile toxin-specific single-domain antibodies / Selection of VHH antibody fragments that recognize different Ab depositions using complex immune libraries / Expression of single-domain antibodies in bacterial systems / Expression of VHHs in Saccharomyces cerevisiae / Stable expression of chimeric heavy chain antibodies in CHO cells / Production of Camel-Like Antibodies in Plants / Selecting and purifying autonomous human variable heavy (VH) domains / Solubility and stability engineering of human VH domains / Improvement of proteolytic stability through in silico engineering / Selection of human VH single domains with improved biophysical properties by phage display / Improvement of single domain antibody stability by disulfide bond introduction / Characterization of single-domain antibodies with an engineered disulfide bond / Affinity maturation of single-domain antibodies by yeast surface display / Multivalent display of single-domain antibodies / Methods for determining the PK parameters of AlbudAbs[TM] and of long serum half-life drugs made using the AlbudAb[TM] technology / Fluorescent protein specific nanotraps to study protein-protein interactions and histone-tail peptide binding / Site-specific labeling of his-tagged nanobodies with 99mTc : a practical guide / Nanobody-based chromatin immunoprecipitation / User-friendly expression plasmids enable the fusion of VHHs to application-specific tags / Application of single-domain antibodies in tumor histochemistry / Nanobodies as structural probes of protein misfolding and fibril formation / Molecular imaging using nanobodies : a case study / Case study on live cell apoptosis-assay using lamin-chromobody cell-lines for high-content analysis / Jean-Luc Teillaud -- Cécile Vincke and Serge Muyldermans -- Stewart D. Nuttall -- Olga Ignatovich [and others] -- Aurelien Olichon and Ario de Marco -- Peter Verheesen and Toon Laeremans -- Jyothi Kumaran, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi -- Kristof De Schutter and Nico Callewaert -- Mireille Pellis, Serge Muyldermans, and Cécile Vincke -- Tomoyuki Tanaka and Terence H. Rabbitts -- Tomoyuki Tanaka and Terence H. Rabbitts -- Armin Sepp and Andrew Griffiths -- Edward Dolk, Theo Verrips, and Hans de Haard -- Greg Hussack [and others] -- Rinse Klooster, Kim S. Rutgers, and Silvere M. van der Maarel -- Toya Nath Baral and Mehdi Arbabi-Ghahroudi -- Andrea Gorlani, Hans de Haard, and Theo Verrips -- Vishal Agrawal [and others] -- Sylvie De Buck [and others] -- Raffi Tonikian and Sachdev S. Sidhu -- Dae Young Kim, Wen Ding, and Jamshid Tanha -- Lucy Rutten, Hans de Haard, and Theo Verrips -- Kip Dudgeon [and others] -- Yoshihisa Hagihara and Dirk Saerens -- Greg Hussack, C. Roger MacKenzie, and Jamshid Tanha -- Akiko Koide and Shohei Koide -- Jianbing Zhang and C. Roger MacKenzie -- Daniel Rycroft and Lucy J. Holt -- Garwin Pichler, Heinrich Leonhardt, and Ulrich Rothbauer -- Catarina Xavier [and others] -- Trong Nguyen Duc [and others] -- Ario de Marco -- Kien T. Maik and C. Roger MacKenzie -- Erwin De Genst and Christopher M. Dobson -- Nick Devoogdt [and others] -- Kourosh Zolghadr [and others].
The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
English.
9781617799686 1617799688 161779967X 9781617799679
10.1007/978-1-61779-968-6 doi
101592924 DNLM
Monoclonal antibodies--Laboratory manuals.
Monoclonal antibodies.
Antibodies, Monoclonal
Biology.
Health & Biological Sciences.
Microbiology & Immunology.
Anticorps monoclonaux--Manuels de laboratoire.
Anticorps monoclonaux.
Monoclonal antibodies
Biology.
Health & Biological Sciences.
Microbiology & Immunology.
Medicine. Immunology. Monoclonal antibodies. Biomedicine. Antibodies. biomedische wetenschappen immunologie antilichamen Medicine (General) Geneeskunde (algemeen)
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QR186.85 / .S56 2012
616.07/98
W1 / ME9616J v.911 2012 QW 525
Includes bibliographical references and index.
From whole monoclonal antibodies to single domain antibodies : think small / Introduction to heavy chain antibodies and derived nanobodies / Overview and discovery of IgNARs and generation of VNARs / Creation of the large and highly functional synthetic repertoire of human VH and Vk domain antibodies / Preparation of a naive library of camelid single domain antibodies / Selection by phage display of single domain antibodies specific to antigens in their native conformation / Semiautomated panning of naive camelidae libraries and selection of single-domain antibodies against peptide antigens / Pichia surface display : a tool for screening single domain antibodies / Bacterial two hybrid : a versatile one-step intracellular selection method / Intracellular antibody capture (IAC) methods for single domain antibodies / Selection of functional single domain antibody fragments for interfering with protein-protein interactions inside cells : a 'one plasmid' mammalian two-hybrid system / Cell-free selection of domain antibodies by in vitro compartmentalization / Selection of VHHs under application conditions / Isolation and characterization of Clostridium difficile toxin-specific single-domain antibodies / Selection of VHH antibody fragments that recognize different Ab depositions using complex immune libraries / Expression of single-domain antibodies in bacterial systems / Expression of VHHs in Saccharomyces cerevisiae / Stable expression of chimeric heavy chain antibodies in CHO cells / Production of Camel-Like Antibodies in Plants / Selecting and purifying autonomous human variable heavy (VH) domains / Solubility and stability engineering of human VH domains / Improvement of proteolytic stability through in silico engineering / Selection of human VH single domains with improved biophysical properties by phage display / Improvement of single domain antibody stability by disulfide bond introduction / Characterization of single-domain antibodies with an engineered disulfide bond / Affinity maturation of single-domain antibodies by yeast surface display / Multivalent display of single-domain antibodies / Methods for determining the PK parameters of AlbudAbs[TM] and of long serum half-life drugs made using the AlbudAb[TM] technology / Fluorescent protein specific nanotraps to study protein-protein interactions and histone-tail peptide binding / Site-specific labeling of his-tagged nanobodies with 99mTc : a practical guide / Nanobody-based chromatin immunoprecipitation / User-friendly expression plasmids enable the fusion of VHHs to application-specific tags / Application of single-domain antibodies in tumor histochemistry / Nanobodies as structural probes of protein misfolding and fibril formation / Molecular imaging using nanobodies : a case study / Case study on live cell apoptosis-assay using lamin-chromobody cell-lines for high-content analysis / Jean-Luc Teillaud -- Cécile Vincke and Serge Muyldermans -- Stewart D. Nuttall -- Olga Ignatovich [and others] -- Aurelien Olichon and Ario de Marco -- Peter Verheesen and Toon Laeremans -- Jyothi Kumaran, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi -- Kristof De Schutter and Nico Callewaert -- Mireille Pellis, Serge Muyldermans, and Cécile Vincke -- Tomoyuki Tanaka and Terence H. Rabbitts -- Tomoyuki Tanaka and Terence H. Rabbitts -- Armin Sepp and Andrew Griffiths -- Edward Dolk, Theo Verrips, and Hans de Haard -- Greg Hussack [and others] -- Rinse Klooster, Kim S. Rutgers, and Silvere M. van der Maarel -- Toya Nath Baral and Mehdi Arbabi-Ghahroudi -- Andrea Gorlani, Hans de Haard, and Theo Verrips -- Vishal Agrawal [and others] -- Sylvie De Buck [and others] -- Raffi Tonikian and Sachdev S. Sidhu -- Dae Young Kim, Wen Ding, and Jamshid Tanha -- Lucy Rutten, Hans de Haard, and Theo Verrips -- Kip Dudgeon [and others] -- Yoshihisa Hagihara and Dirk Saerens -- Greg Hussack, C. Roger MacKenzie, and Jamshid Tanha -- Akiko Koide and Shohei Koide -- Jianbing Zhang and C. Roger MacKenzie -- Daniel Rycroft and Lucy J. Holt -- Garwin Pichler, Heinrich Leonhardt, and Ulrich Rothbauer -- Catarina Xavier [and others] -- Trong Nguyen Duc [and others] -- Ario de Marco -- Kien T. Maik and C. Roger MacKenzie -- Erwin De Genst and Christopher M. Dobson -- Nick Devoogdt [and others] -- Kourosh Zolghadr [and others].
The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
English.
9781617799686 1617799688 161779967X 9781617799679
10.1007/978-1-61779-968-6 doi
101592924 DNLM
Monoclonal antibodies--Laboratory manuals.
Monoclonal antibodies.
Antibodies, Monoclonal
Biology.
Health & Biological Sciences.
Microbiology & Immunology.
Anticorps monoclonaux--Manuels de laboratoire.
Anticorps monoclonaux.
Monoclonal antibodies
Biology.
Health & Biological Sciences.
Microbiology & Immunology.
Medicine. Immunology. Monoclonal antibodies. Biomedicine. Antibodies. biomedische wetenschappen immunologie antilichamen Medicine (General) Geneeskunde (algemeen)
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QR186.85 / .S56 2012
616.07/98
W1 / ME9616J v.911 2012 QW 525