Spermatogenesis : methods and protocols /
Spermatogenesis : methods and protocols /
edited by Douglas T. Carrell, Kenneth I. Aston.
- New York : Humana Press, ©2013.
- 1 online resource (xvii, 554 pages) : illustrations (some color)
- Methods in molecular biology, 927 1940-6029 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 927. .
Includes bibliographical references and index.
Methods for sperm concentration determination / Methods of sperm vitality assessment / Hypo-osmotic swelling test for evaluation of sperm membrane integrity / Sperm morphology classification : a rational method for schemes adopted by the World Health Organization / Sperm morphology assessment using strict (tygerberg) criteria / Methods for direct and indirect antisperm antibody testing / Hiroaki Shibahara and Junko Koriyama -- Manual methods for sperm motility assessment / David Mortimer and Sharon T. Mortimer -- Computer-aided sperm analysis (CASA) of sperm motility and hyperactivation / Hemizona assay for assessment of sperm function / Sperm penetration assay for the assessment of fertilization capacity / Methods for the assessment of sperm capacitation and acrosome reaction excluding the sperm penetration assay / Sperm DNA fragmentation analysis using the TUNEL assay / Sperm DNA damage measured by comet assay / Sperm chromatin structure assay (SCSA) / Sperm aneuploidy testing using fluorescence in situ hybridization / Flow cytometric methods for sperm assessment / Human y chromosome microdeletion analysis by PCR multiplex protocols identifying only clinically relevant AZF microdeletions / Sperm cryopreservation methods / Density gradient separation of sperm for artificial insemination / Recovery, isolation, identification, and preparation of spermatozoa from human testis / Enhancement of sperm motility using pentoxifylline and platelet-activating factor / Intracytoplasmic morphology-selected sperm injection / Sperm selection for ICSI using annexin V / Sperm selection for ICSI using the hyaluronic acid binding assay / sperm selection based on electrostatic charge / Sex-sorting sperm using flow cytometry/cell sorting / Assessment of spermatogenesis through staging of seminiferous tubules / Immunohistochemical approaches for the study of spermatogenesis / Ultrastructural analysis of testicular tissue and sperm by transmission and scanning electron microscopy / Assessment of oxidative stress in sperm and semen / Improved chemiluminescence assay for measuring antioxidant capacity of seminal plasma / Methods of sperm DNA extraction for genetic and epigenetic studies / Isolating mRNA and small noncoding RNAs from human sperm / Review of genome-wide approaches to study the genetic basis for spermatogenic defects / Methods for the analysis of the sperm proteome / Methodology of aniline blue staining of chromatin and the assessment of the associated nuclear and cytoplasmic attributes in human sperm / Isolation of sperm nuclei and nuclear matrices from the mouse, and other Rodents / Protamine extraction and analysis of human sperm protamine 1/protamine 2 ratio using acid gel electrophoresis / Analysis of gene-specific and genome-wide sperm DNA methylation / Evaluating the localization and DNA binding complexity of histones in mature sperm / In vitro spermatogenesis using an organ culture technique / Testicular tissue grafting and male germ cell transplantation / Transgenic modification of spermatogonial stem cells using lentiviral vectors / Methods for sperm-mediated gene transfer / Phenotypic sssessment of male fertility status in transgenic animal models / Lars Bjorndahl -- Sergey I. Moskovtsev and Clifford L. Librach -- Sivakumar Ramu and Rajasingam S. Jeyendran -- Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow -- Roelof Menkveld -- David Mortimer and Sharon T. Mortimer -- Sergio Oehninger, Mahmood Morshedi, and Daniel Franken -- Kathleen Hwang and Dolores J. Lamb -- Christopher J. De Jonge and Christopher L.R. Barratt -- Rakesh Sharma, Jayson Masaki, and Ashok Agarwal -- Luke Simon and Douglas T. Carrell -- Donald P. Evenson -- Benjamin R. Emery -- Vanesa Robles and Felipe Martinez-Pastor -- Peter H. Vogt and Ulrike Bender -- Tiffany Justice and Greg Christensen -- David Mortimer and Sharon T. Mortimer -- Charles H. Muller and Erin R. Pagel -- Shilo L. Archer and William E. Roudebush -- Luke Simon, Aaron Wilcox, and Douglas T. Carrell -- Sonja Grunewald and Uwe Paasch -- Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh -- Luke Simon, Shao-qin Ge, and Douglas T. Carrell -- Duane L. Garner, K. Michael Evans, and George E. Seidel -- Marvin L. Meistrich and Rex A. Hess -- Cathryn A. Hogarth and Michael D. Griswold -- Hector E. Chemes -- Anthony H. Kashou, Rakesh Sharma, and Ashok Agarwal -- Charles H. Muller, Tiffany K.Y. Lee, and Michalina A. Montano -- Jeanine Griffin -- Robert J. Goodrich, Ester Anton, and Stephen A. Krawetz -- Kenneth I. Aston and Donald F. Conrad -- Sara de Mateo, Josep Maria Estanyol, and Rafael Oliva -- Leyla Sati and Gabor Huszar -- W. Steven Ward -- Lihua Liu, Kenneth I. Aston, and Douglas T. Carrell -- Saher Sue Hammoud, Bradley R. Cairns, and Douglas T. Carrell -- David Miller and Agnieszka Paradowska -- Tetsuhiro Yokonishi, Takuya Sato, Kumiko Katagiri, and Takehiko Ogawa -- Jose R. Rodriguez-Sosa, Lin Tang, and Ina Dobrinski -- Christina Tenenhaus Dann -- Marialuisa Lavitrano, Roberto Giovannoni, and Maria Grazia Cerrito -- David M. de Kretser and Liza O'Donnell.
Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.
English.
9781627030380 1627030387
10.1007/978-1-62703-038-0. doi
Spermatogenesis--Laboratory manuals.
Spermatogenesis.
Spermatogenesis
Spermatogenèse--Manuels de laboratoire.
Spermatogenèse.
Spermatogenesis
Life sciences. Cytology. Developmental biology. Embryology. Cell Biology. spermatogenese spermatogenesis celbiologie cellular biology mechatronica mechatronics ontogenie ontogeny gametogenese gametogenesis Ontogeny, Embryology Ontogenie, embryologie
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QL966 / .S64 2013
571.8/451
WJ 834
Includes bibliographical references and index.
Methods for sperm concentration determination / Methods of sperm vitality assessment / Hypo-osmotic swelling test for evaluation of sperm membrane integrity / Sperm morphology classification : a rational method for schemes adopted by the World Health Organization / Sperm morphology assessment using strict (tygerberg) criteria / Methods for direct and indirect antisperm antibody testing / Hiroaki Shibahara and Junko Koriyama -- Manual methods for sperm motility assessment / David Mortimer and Sharon T. Mortimer -- Computer-aided sperm analysis (CASA) of sperm motility and hyperactivation / Hemizona assay for assessment of sperm function / Sperm penetration assay for the assessment of fertilization capacity / Methods for the assessment of sperm capacitation and acrosome reaction excluding the sperm penetration assay / Sperm DNA fragmentation analysis using the TUNEL assay / Sperm DNA damage measured by comet assay / Sperm chromatin structure assay (SCSA) / Sperm aneuploidy testing using fluorescence in situ hybridization / Flow cytometric methods for sperm assessment / Human y chromosome microdeletion analysis by PCR multiplex protocols identifying only clinically relevant AZF microdeletions / Sperm cryopreservation methods / Density gradient separation of sperm for artificial insemination / Recovery, isolation, identification, and preparation of spermatozoa from human testis / Enhancement of sperm motility using pentoxifylline and platelet-activating factor / Intracytoplasmic morphology-selected sperm injection / Sperm selection for ICSI using annexin V / Sperm selection for ICSI using the hyaluronic acid binding assay / sperm selection based on electrostatic charge / Sex-sorting sperm using flow cytometry/cell sorting / Assessment of spermatogenesis through staging of seminiferous tubules / Immunohistochemical approaches for the study of spermatogenesis / Ultrastructural analysis of testicular tissue and sperm by transmission and scanning electron microscopy / Assessment of oxidative stress in sperm and semen / Improved chemiluminescence assay for measuring antioxidant capacity of seminal plasma / Methods of sperm DNA extraction for genetic and epigenetic studies / Isolating mRNA and small noncoding RNAs from human sperm / Review of genome-wide approaches to study the genetic basis for spermatogenic defects / Methods for the analysis of the sperm proteome / Methodology of aniline blue staining of chromatin and the assessment of the associated nuclear and cytoplasmic attributes in human sperm / Isolation of sperm nuclei and nuclear matrices from the mouse, and other Rodents / Protamine extraction and analysis of human sperm protamine 1/protamine 2 ratio using acid gel electrophoresis / Analysis of gene-specific and genome-wide sperm DNA methylation / Evaluating the localization and DNA binding complexity of histones in mature sperm / In vitro spermatogenesis using an organ culture technique / Testicular tissue grafting and male germ cell transplantation / Transgenic modification of spermatogonial stem cells using lentiviral vectors / Methods for sperm-mediated gene transfer / Phenotypic sssessment of male fertility status in transgenic animal models / Lars Bjorndahl -- Sergey I. Moskovtsev and Clifford L. Librach -- Sivakumar Ramu and Rajasingam S. Jeyendran -- Susan A. Rothmann, Anna-Marie Bort, John Quigley, and Robin Pillow -- Roelof Menkveld -- David Mortimer and Sharon T. Mortimer -- Sergio Oehninger, Mahmood Morshedi, and Daniel Franken -- Kathleen Hwang and Dolores J. Lamb -- Christopher J. De Jonge and Christopher L.R. Barratt -- Rakesh Sharma, Jayson Masaki, and Ashok Agarwal -- Luke Simon and Douglas T. Carrell -- Donald P. Evenson -- Benjamin R. Emery -- Vanesa Robles and Felipe Martinez-Pastor -- Peter H. Vogt and Ulrike Bender -- Tiffany Justice and Greg Christensen -- David Mortimer and Sharon T. Mortimer -- Charles H. Muller and Erin R. Pagel -- Shilo L. Archer and William E. Roudebush -- Luke Simon, Aaron Wilcox, and Douglas T. Carrell -- Sonja Grunewald and Uwe Paasch -- Mohammad Hossein Nasr-Esfahani and Tavalaee Marziyeh -- Luke Simon, Shao-qin Ge, and Douglas T. Carrell -- Duane L. Garner, K. Michael Evans, and George E. Seidel -- Marvin L. Meistrich and Rex A. Hess -- Cathryn A. Hogarth and Michael D. Griswold -- Hector E. Chemes -- Anthony H. Kashou, Rakesh Sharma, and Ashok Agarwal -- Charles H. Muller, Tiffany K.Y. Lee, and Michalina A. Montano -- Jeanine Griffin -- Robert J. Goodrich, Ester Anton, and Stephen A. Krawetz -- Kenneth I. Aston and Donald F. Conrad -- Sara de Mateo, Josep Maria Estanyol, and Rafael Oliva -- Leyla Sati and Gabor Huszar -- W. Steven Ward -- Lihua Liu, Kenneth I. Aston, and Douglas T. Carrell -- Saher Sue Hammoud, Bradley R. Cairns, and Douglas T. Carrell -- David Miller and Agnieszka Paradowska -- Tetsuhiro Yokonishi, Takuya Sato, Kumiko Katagiri, and Takehiko Ogawa -- Jose R. Rodriguez-Sosa, Lin Tang, and Ina Dobrinski -- Christina Tenenhaus Dann -- Marialuisa Lavitrano, Roberto Giovannoni, and Maria Grazia Cerrito -- David M. de Kretser and Liza O'Donnell.
Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.
English.
9781627030380 1627030387
10.1007/978-1-62703-038-0. doi
Spermatogenesis--Laboratory manuals.
Spermatogenesis.
Spermatogenesis
Spermatogenèse--Manuels de laboratoire.
Spermatogenèse.
Spermatogenesis
Life sciences. Cytology. Developmental biology. Embryology. Cell Biology. spermatogenese spermatogenesis celbiologie cellular biology mechatronica mechatronics ontogenie ontogeny gametogenese gametogenesis Ontogeny, Embryology Ontogenie, embryologie
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QL966 / .S64 2013
571.8/451
WJ 834