Cryopreservation and freeze-drying protocols /
Cryopreservation and freeze-drying protocols /
edited by Willem F. Wolkers, Harriette Oldenhof.
- Third edition.
- 1 online resource (xiii, 509 pages) : illustrations (some color)
- Methods in molecular biology, 1257 1940-6029 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1257. .
Includes bibliographical references and index.
Principles of cryopreservation / Principles of cryopreservation by vitrification / Modeling and optimization of cryopreservation / Principles of freeze-drying / Use of in situ fourier transform infrared spectroscopy to study freezing and drying of cells / Calorimetric analysis of cryopreservation and freeze-drying formulations / Measurement of intracellular ice formation kinetics by high-speed video cryomicroscopy / Laser scanning microscopy in cryobiology / Low-temperature electron microscopy : techniques and protocols / Cryopreservation of semen from domestic livestock / Cryopreservation of mammalian oocytes / Vitrification : a simple and successful method for cryostorage of human blastocysts / Efficient cryopreservation of human pluripotent stem cells by surface-based vitrification / Cryopreservation of Greenshell mussel (Perna canaliculus) sperm / Membrane modification strategies for cryopreservation / Sperm cleanup and centrifugation processing for cryopreservation / Cryopreservation of red blood cells / Cord blood clinical processing, cryopreservation, and storage / Directional freezing for large volume cryopreservation / Vitrification of heart valve tissues / Cryopreservation of plant cell lines / Writing standard operating procedures (SOPs) for cryostorage protocols : using shoot meristem cryopreservation as an example / Freeze-drying of proteins / Freeze-drying of lactic acid Bbacteria / Freeze-drying of mammalian sperm / Freeze-drying of decellularized deart valve tissues / David E. Pegg -- Gregory M. Fahy and Brian Wowk -- James D. Benson -- Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward -- Willem F. Wolkers and Harriëtte Oldenhof -- Wendell Q. Sun -- Jens O.M. Karlsson -- Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann -- Roland A. Fleck -- Harald Sieme and Harriëtte Oldenhof -- Victoria Keros and Barry J. Fuller -- Juergen Liebermann -- Julia C. Neubauer [and 3 others] -- Serean L. Adams [and 4 others] -- Phillip H. Purdy and James K. Graham -- Harald Sieme and Harriëtte Oldenhof -- Johan W. Lagerberg -- Heidi Elmoazzen and Jelena L. Holovati -- Joseph Saragusty -- Kelvin G.M. Brockbank [and 3 others] -- Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack -- Keith Harding and Erica E. Benson -- Baolin Liu and Xinli Zhou -- Fernanda Fonseca, Stéphanie Cenard, and Stéphanie Passot -- Levent Keskintepe and Ali Eroglu -- Willem F. Wolkers and Andres Hilfiker.
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
9781493921935 1493921932 1493921924 9781493921928
com.springer.onix.9781493921935 Springer Nature
GBB8L6732 bnb
019127181 Uk
Cryopreservation of organs, tissues, etc.--Methods.
Freeze-drying--Methods.
Cryopreservation--methods
Freeze Drying--methods
Materials science.
Biotechnology.
Technology & Engineering--Material Science.
Science--Biotechnology.
Cryopreservation of organs, tissues, etc.
Freeze-drying
Laboratory Manual
Methods (Music)
Laboratory manuals.
Manuels de laboratoire.
TP248.25.C79 TP248.13-248.65
660.6
Includes bibliographical references and index.
Principles of cryopreservation / Principles of cryopreservation by vitrification / Modeling and optimization of cryopreservation / Principles of freeze-drying / Use of in situ fourier transform infrared spectroscopy to study freezing and drying of cells / Calorimetric analysis of cryopreservation and freeze-drying formulations / Measurement of intracellular ice formation kinetics by high-speed video cryomicroscopy / Laser scanning microscopy in cryobiology / Low-temperature electron microscopy : techniques and protocols / Cryopreservation of semen from domestic livestock / Cryopreservation of mammalian oocytes / Vitrification : a simple and successful method for cryostorage of human blastocysts / Efficient cryopreservation of human pluripotent stem cells by surface-based vitrification / Cryopreservation of Greenshell mussel (Perna canaliculus) sperm / Membrane modification strategies for cryopreservation / Sperm cleanup and centrifugation processing for cryopreservation / Cryopreservation of red blood cells / Cord blood clinical processing, cryopreservation, and storage / Directional freezing for large volume cryopreservation / Vitrification of heart valve tissues / Cryopreservation of plant cell lines / Writing standard operating procedures (SOPs) for cryostorage protocols : using shoot meristem cryopreservation as an example / Freeze-drying of proteins / Freeze-drying of lactic acid Bbacteria / Freeze-drying of mammalian sperm / Freeze-drying of decellularized deart valve tissues / David E. Pegg -- Gregory M. Fahy and Brian Wowk -- James D. Benson -- Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward -- Willem F. Wolkers and Harriëtte Oldenhof -- Wendell Q. Sun -- Jens O.M. Karlsson -- Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann -- Roland A. Fleck -- Harald Sieme and Harriëtte Oldenhof -- Victoria Keros and Barry J. Fuller -- Juergen Liebermann -- Julia C. Neubauer [and 3 others] -- Serean L. Adams [and 4 others] -- Phillip H. Purdy and James K. Graham -- Harald Sieme and Harriëtte Oldenhof -- Johan W. Lagerberg -- Heidi Elmoazzen and Jelena L. Holovati -- Joseph Saragusty -- Kelvin G.M. Brockbank [and 3 others] -- Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack -- Keith Harding and Erica E. Benson -- Baolin Liu and Xinli Zhou -- Fernanda Fonseca, Stéphanie Cenard, and Stéphanie Passot -- Levent Keskintepe and Ali Eroglu -- Willem F. Wolkers and Andres Hilfiker.
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
9781493921935 1493921932 1493921924 9781493921928
com.springer.onix.9781493921935 Springer Nature
GBB8L6732 bnb
019127181 Uk
Cryopreservation of organs, tissues, etc.--Methods.
Freeze-drying--Methods.
Cryopreservation--methods
Freeze Drying--methods
Materials science.
Biotechnology.
Technology & Engineering--Material Science.
Science--Biotechnology.
Cryopreservation of organs, tissues, etc.
Freeze-drying
Laboratory Manual
Methods (Music)
Laboratory manuals.
Manuels de laboratoire.
TP248.25.C79 TP248.13-248.65
660.6