Plant synthetic promoters : methods and protocols /
Plant synthetic promoters : methods and protocols /
edited by Reinhard Hehl.
- 1 online resource (xi, 325 pages) : illustrations (some color)
- Methods in molecular biology, volume 1482 1064-3745 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1482. .
Includes index.
Includes bibliographical references and index.
What Have We Learned About Synthetic Promoter Construction? -- Quantitative Analysis of Cis-Regulatory Element Activity Using Synthetic Promoters in Transgenic Plants -- The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information -- Quantitative Analysis of Protein-DNA Interaction by qDPI-ELISA -- Analyzing Synthetic Promoters Using Arabidopsis Protoplasts -- Selecting Hypomethylated Genomic Regions Using MRE-Seq -- Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues -- Novel Synthetic Promoters from the Cestrum Yellow Leaf-Curling Virus -- Fast and Efficient Cloning of Cis-Regulatory Sequences for High Throughput Yeast One-Hybrid Analyses of Transcription Factors -- The Physcomitrella patens System for Transient Gene Expression Assays -- Analysis of Microbe-Associated Molecular Pattern-Responsive Synthetic Promoters with the Parsley Protoplast System -- A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules -- Simultaneous Analysis of Multiple Promoters: An Application of the PC-GW Binary Vector Series -- GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis -- Bioinformatic Identification of Conserved Cis-Sequences in Coregulated Genes -- In Silico Expression Analysis -- FootprintDB: Analysis of Plant Cis-Regulatory Elements, Transcription Factors, and Binding Interfaces -- RSAT::Plants: Motif Discovery Within Clusters of Upstream Sequences in Plant Genomes -- RSAT::Plants: Motif Discovery in ChIP-Seq Peaks of Plant Genomes.
This text assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis -regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented.
9781493963966 1493963961 1493963945 9781493963942
9781493963942 10.1007/978-1-4939-6396-6 doi
10.1007/978-1-4939-6396-6 00012511
GBB8M4863 bnb
101694475 DNLM 019140401 Uk
Plant gene expression.
Plant genetic transformation.
Promoters (Genetics)
Genetics--Technique.
Gene Expression Regulation, Plant
Promoter Regions, Genetic
Plants--genetics
Regulatory Elements, Transcriptional
Genetic Techniques
Plantes--Expression génique.
Transformation génétique végétale.
Promoteurs (Génétique)
Génétique--Technique.
Botany & plant sciences.
Science--Life Sciences--Botany.
Promoters (Genetics)
Genetics--Technique
Plant gene expression
Plant genetic transformation
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QK981
572.8/652
W1 / ME9616J v.1482 2016 QK 981
Includes index.
Includes bibliographical references and index.
What Have We Learned About Synthetic Promoter Construction? -- Quantitative Analysis of Cis-Regulatory Element Activity Using Synthetic Promoters in Transgenic Plants -- The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information -- Quantitative Analysis of Protein-DNA Interaction by qDPI-ELISA -- Analyzing Synthetic Promoters Using Arabidopsis Protoplasts -- Selecting Hypomethylated Genomic Regions Using MRE-Seq -- Spatio-Temporal Imaging of Promoter Activity in Intact Plant Tissues -- Novel Synthetic Promoters from the Cestrum Yellow Leaf-Curling Virus -- Fast and Efficient Cloning of Cis-Regulatory Sequences for High Throughput Yeast One-Hybrid Analyses of Transcription Factors -- The Physcomitrella patens System for Transient Gene Expression Assays -- Analysis of Microbe-Associated Molecular Pattern-Responsive Synthetic Promoters with the Parsley Protoplast System -- A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules -- Simultaneous Analysis of Multiple Promoters: An Application of the PC-GW Binary Vector Series -- GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis -- Bioinformatic Identification of Conserved Cis-Sequences in Coregulated Genes -- In Silico Expression Analysis -- FootprintDB: Analysis of Plant Cis-Regulatory Elements, Transcription Factors, and Binding Interfaces -- RSAT::Plants: Motif Discovery Within Clusters of Upstream Sequences in Plant Genomes -- RSAT::Plants: Motif Discovery in ChIP-Seq Peaks of Plant Genomes.
This text assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis -regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented.
9781493963966 1493963961 1493963945 9781493963942
9781493963942 10.1007/978-1-4939-6396-6 doi
10.1007/978-1-4939-6396-6 00012511
GBB8M4863 bnb
101694475 DNLM 019140401 Uk
Plant gene expression.
Plant genetic transformation.
Promoters (Genetics)
Genetics--Technique.
Gene Expression Regulation, Plant
Promoter Regions, Genetic
Plants--genetics
Regulatory Elements, Transcriptional
Genetic Techniques
Plantes--Expression génique.
Transformation génétique végétale.
Promoteurs (Génétique)
Génétique--Technique.
Botany & plant sciences.
Science--Life Sciences--Botany.
Promoters (Genetics)
Genetics--Technique
Plant gene expression
Plant genetic transformation
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QK981
572.8/652
W1 / ME9616J v.1482 2016 QK 981