ATM kinase : methods and protocols /
ATM kinase : methods and protocols /
edited by Sergei V. Kozlov.
- 1 online resource (xiv, 439 pages) : illustrations (some color)
- Methods in molecular biology, 1599 1064-3745 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1599. .
Includes bibliographical references and index.
Assaying radiosensitivity of ataxia-telangiectasia / Assaying for radioresistant DNA synthesis, the hallmark feature of the intra-S-phase checkpoint using a DNA fiber technique / ATM gene mutation detection techniques and functional analysis / HTRF assay for the protein kinase ATM / ATM kinase inhibitors : HTS cellular imaging assay using cellomics ArrayScan VTI platform / Image-based high content screening : automating the quantification process for DNA damage-induced foci / Analyzing ATM function by electroporation of endonucleases and immunofluorescence microscopy / Quantitative and dynamic imaging of ATM kinase activity by bioluminescence imaging / Zn(II)-Phos-Tag SDS-PAGE for separation and detection of a DNA damage-related signaling large phosphoprotein / Identification of ATM protein kinase phosphorylation sites by mass spectrometry / Studies of ATM kinase activity using engineered ATM sensitive to ATP analogues (ATM-AS) / Functional characterization of ATM kinase using acetylation-specific antibodies / Identification of ATM-interacting proteins by co-immunoprecipitation and Glutathione-S-Transferase (GST) pull-down assays / ATM activation and H2AX phosphorylation induced by genotoxic agents assessed by flow- and laser scanning cytometry / Peptide immunoaffinity enrichment with targeted mass spectrometry : application to quantification of ATM kinase phospho-signaling / Mass spectrometry-based proteomics for quantifying DNA damage-induced phosphorylation / Statistical analysis of ATM-dependent signaling in quantitative mass spectrometry phosphoproteomics / ChIP technique to study protein dynamics at defined DNA double strand breaks / Studies of the DNA damage response by using the Lac Operator/Repressor (LacO/LacR) tethering system / Imaging of fluorescently tagged ATM kinase at the sites of DNA double strand breaks / Live cell imaging to study real-time ATM-mediated recruitment of DNA repair complexes to sites of ionizing radiation-induced DNA damage / Analyzing heterochromatic DNA double strand break (DSB) repair in response to ionizing radiation / Phenotypic analysis of ATM protein kinase in DNA double-strand break formation and repair / Monitoring DNA repair consequences of ATM signaling using simultaneous fluorescent readouts / Noncanonical ATM activation and signaling in response to transcription-blocking DNA damage / Study of ATM phosphorylation by Cdk5 in neuronal cells / DNA damage response in human stem cells and neural descendants / Patient-specific stem cell model to investigate the neurological phenotype observed in ataxia-telangiectasia / Lentiviral reprogramming of A-T patient fibroblasts to induced pluripotent stem cells / Monitoring the ATM-mediated DNA damage response in the cerebellum using organotypic cultures / Hailiang Hu, Shareef Nahas, and Richard A. Gatti -- Amanda W. Kijas and Martin F. Lavin -- Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern -- Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater -- Catherine Bardelle and Joanna Boros -- Yi Chieh Lim -- Keiji Suzuki -- Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla -- Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike -- Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov -- Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo -- Yingli Sun and Fengxia Du -- Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna -- Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz -- Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich -- Marina E. Borisova, Sebastian A. Wagner, and Petra Beli -- Ashley J. Waardenberg -- Jie Wen and Patrick Concannon -- Rossana Piccinno, Marta Cipinska, and Vassilis Roukos -- Anthony J. Davis, Shih-Ya Wang, David J. Chen, and Benjamin P.C. Chen -- Burkhard Jakob and Gisela Taucher-Scholz -- Karolin Klement and Aaron A. Goodarzi -- Elisabeth Mian and Lisa Wiesmüller -- Adrian Wiegmans -- Jurgen A. Marteijn, Wim Vermeulen, and Maria Tresini -- Hua She and Zixu Mao -- Jason M. Beckta, Bret R. Adams, and Kristoffer Valerie -- Romal Stewart, Gautam Wali, Chris Perry, Martin F. Lavin, Francois Féron, Alan Mackay-Sim, and Ratneswary Sutharsan -- Sam Nayler, Sergei V. Kozlov, Martin F. Lavin, and Ernst Wolvetang -- Efrat Tal and Yosef Shiloh.
Ataxia-telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successfulMethods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system. Comprehensive and practical, ATM Kinase: Methods and Protocols aims to ignite and attract the interest of colleagues from diverse fields to A-T research in an effort to bring their expertise and fresh ideas to resolve many A-T puzzles still waiting to be pieced together and to alleviate the suffering of A-T children and their families.
9781493969555 1493969552 1493969536 9781493969531
10.1007/978-1-4939-6955-5 doi 9781493969531
9781493969531 00012511
GBB8M4912 bnb
101712326 DNLM 019140455 Uk
Ataxia telangiectasia--Genetic aspects--Laboratory manuals.
Protein kinases--Laboratory manuals.
DNA damage.
Protein kinases.
Ataxia Telangiectasia Mutated Proteins--genetics
DNA Damage
Protein Kinases
Ataxie-télangiectasie--Aspect génétique--Manuels de laboratoire.
Protéines kinases--Manuels de laboratoire.
ADN--Lésions.
Protéines kinases.
Medical genetics.
Medical--Genetics.
DNA damage
Protein kinases
Electronic books.
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QP606.P76
572/.6
W1 / ME9616J v.1599 2017 QU 25
Includes bibliographical references and index.
Assaying radiosensitivity of ataxia-telangiectasia / Assaying for radioresistant DNA synthesis, the hallmark feature of the intra-S-phase checkpoint using a DNA fiber technique / ATM gene mutation detection techniques and functional analysis / HTRF assay for the protein kinase ATM / ATM kinase inhibitors : HTS cellular imaging assay using cellomics ArrayScan VTI platform / Image-based high content screening : automating the quantification process for DNA damage-induced foci / Analyzing ATM function by electroporation of endonucleases and immunofluorescence microscopy / Quantitative and dynamic imaging of ATM kinase activity by bioluminescence imaging / Zn(II)-Phos-Tag SDS-PAGE for separation and detection of a DNA damage-related signaling large phosphoprotein / Identification of ATM protein kinase phosphorylation sites by mass spectrometry / Studies of ATM kinase activity using engineered ATM sensitive to ATP analogues (ATM-AS) / Functional characterization of ATM kinase using acetylation-specific antibodies / Identification of ATM-interacting proteins by co-immunoprecipitation and Glutathione-S-Transferase (GST) pull-down assays / ATM activation and H2AX phosphorylation induced by genotoxic agents assessed by flow- and laser scanning cytometry / Peptide immunoaffinity enrichment with targeted mass spectrometry : application to quantification of ATM kinase phospho-signaling / Mass spectrometry-based proteomics for quantifying DNA damage-induced phosphorylation / Statistical analysis of ATM-dependent signaling in quantitative mass spectrometry phosphoproteomics / ChIP technique to study protein dynamics at defined DNA double strand breaks / Studies of the DNA damage response by using the Lac Operator/Repressor (LacO/LacR) tethering system / Imaging of fluorescently tagged ATM kinase at the sites of DNA double strand breaks / Live cell imaging to study real-time ATM-mediated recruitment of DNA repair complexes to sites of ionizing radiation-induced DNA damage / Analyzing heterochromatic DNA double strand break (DSB) repair in response to ionizing radiation / Phenotypic analysis of ATM protein kinase in DNA double-strand break formation and repair / Monitoring DNA repair consequences of ATM signaling using simultaneous fluorescent readouts / Noncanonical ATM activation and signaling in response to transcription-blocking DNA damage / Study of ATM phosphorylation by Cdk5 in neuronal cells / DNA damage response in human stem cells and neural descendants / Patient-specific stem cell model to investigate the neurological phenotype observed in ataxia-telangiectasia / Lentiviral reprogramming of A-T patient fibroblasts to induced pluripotent stem cells / Monitoring the ATM-mediated DNA damage response in the cerebellum using organotypic cultures / Hailiang Hu, Shareef Nahas, and Richard A. Gatti -- Amanda W. Kijas and Martin F. Lavin -- Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern -- Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater -- Catherine Bardelle and Joanna Boros -- Yi Chieh Lim -- Keiji Suzuki -- Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla -- Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike -- Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov -- Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo -- Yingli Sun and Fengxia Du -- Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna -- Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz -- Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich -- Marina E. Borisova, Sebastian A. Wagner, and Petra Beli -- Ashley J. Waardenberg -- Jie Wen and Patrick Concannon -- Rossana Piccinno, Marta Cipinska, and Vassilis Roukos -- Anthony J. Davis, Shih-Ya Wang, David J. Chen, and Benjamin P.C. Chen -- Burkhard Jakob and Gisela Taucher-Scholz -- Karolin Klement and Aaron A. Goodarzi -- Elisabeth Mian and Lisa Wiesmüller -- Adrian Wiegmans -- Jurgen A. Marteijn, Wim Vermeulen, and Maria Tresini -- Hua She and Zixu Mao -- Jason M. Beckta, Bret R. Adams, and Kristoffer Valerie -- Romal Stewart, Gautam Wali, Chris Perry, Martin F. Lavin, Francois Féron, Alan Mackay-Sim, and Ratneswary Sutharsan -- Sam Nayler, Sergei V. Kozlov, Martin F. Lavin, and Ernst Wolvetang -- Efrat Tal and Yosef Shiloh.
Ataxia-telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successfulMethods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system. Comprehensive and practical, ATM Kinase: Methods and Protocols aims to ignite and attract the interest of colleagues from diverse fields to A-T research in an effort to bring their expertise and fresh ideas to resolve many A-T puzzles still waiting to be pieced together and to alleviate the suffering of A-T children and their families.
9781493969555 1493969552 1493969536 9781493969531
10.1007/978-1-4939-6955-5 doi 9781493969531
9781493969531 00012511
GBB8M4912 bnb
101712326 DNLM 019140455 Uk
Ataxia telangiectasia--Genetic aspects--Laboratory manuals.
Protein kinases--Laboratory manuals.
DNA damage.
Protein kinases.
Ataxia Telangiectasia Mutated Proteins--genetics
DNA Damage
Protein Kinases
Ataxie-télangiectasie--Aspect génétique--Manuels de laboratoire.
Protéines kinases--Manuels de laboratoire.
ADN--Lésions.
Protéines kinases.
Medical genetics.
Medical--Genetics.
DNA damage
Protein kinases
Electronic books.
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QP606.P76
572/.6
W1 / ME9616J v.1599 2017 QU 25