Plant protein secretion : methods and protocols /
Plant protein secretion : methods and protocols /
edited by Liwen Jiang.
- 1 online resource (xi, 285 pages) : illustrations (some color.)
- Methods in molecular biology, 1662 1940-6029 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1662. .
Includes bibliographical references and index.
An overview of protein secretion in yeast and animal cells / An overview of protein secretion in plant cells / Bioinformatics analysis of protein secretion in plants / Proteomic analysis of secreted proteins from cell microenvironment / Using homology modeling to understand the structural basis of specific interaction of a plant-specific AtSar1a-AtSec23a pair involved in protein ER export / Analysis of Golgi-mediated protein traffic in plant cells / Analysis of membrane protein topology in the plant secretory pathway / Semiautomatic segmentation of plant Golgi stacks in electron tomograms using 3dmod / 3D printing of plant Golgi stacks from their electron tomographic models / Transient expression of chimeric fluorescent reporter proteins in pollen tubes to study protein polar secretion and dynamics / Analysis of actin-based intracellular trafficking in pollen tubes / Analysis of phragmoplast kinetics during plant cytokinesis / Immunofluorescence analysis of membrane-associated proteins for calthrin-mediated endocytosis in plant root cells / In vivo interaction studies by measuring Förster resonance energy transfer through fluorescence lifetime imaging microscopy (FRET/FLIM) / Analysis of nano-body-epitope interactions in living cells via quantitative protein transport assays / A secretion system for cargo protein identification of vacuolar sorting receptors / Identifying novel regulators of vacuolar trafficking by combining fluorescence imaging-based forward genetic screening and in vitro pollen germination / Measuring plan protein secretion / Transient secretory enzyme expression in leaf protoplasts to characterize SNARE functional classes in conventional and unconventional secretion / The organelle pH estimate and measurement in plant secratory pathway / Analysis of exocyst-positive organelle (EXPO)-mediated unconventional protein secretion (UPS) in plant cells / Isolation of the plant exocyst complex / Using microscopy tools to visualize autophagosomal structures in plant cells / Analysis of plant autophagy / Yusong Guo, Feng Yang, Xiao Tang -- Kin Pau Chung, Yonglun Zeng -- Liyuan Chen -- Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian -- Yap-Shing Nim, Shuangli Sun, Kam-Bo Wong -- Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao -- Jinya Guo, Yansong Miao, Yi Cai -- Keith Ka Ki Mai, Byung-Ho Kang -- Keith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang -- Guitao Zhong, Ronghe Liu, Menglong Zhuang, Hao Wang -- Yuxiang Jiang, Meng Zhang, Shanjin Huang -- Pantelis Livanos, Mayank Chugh, Sabine Müller -- Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, Jianwei Pan -- Florian Fässler, Peter Pimpl -- Simone Früholz and Peter Pimpl -- Jinbo Shen -- Qiang-Nan Feng, Yan Zhang -- Emily R. Larson -- 19. Di Sansebastiano Gian Pietro, Barozzi Fabrizio -- Jinbo Shen -- Yu Ding, Juan Wang -- King Pong Leung, Wilson Chun Yu Lau -- Weili Lin, Xiaohong Zhuang -- Liang Chen, Faqiang Li, Shi Xiao. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 20. 21. 22. 23. 24.
This volume aims to provide an update on recent developments in protein secretion studies in plants versus yeast and mammalian systems. This book also discusses case studies that analyze the use of plant protein secretion using various tools and systems. The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Protein Secretion: Methods and Protocols is a valuable resource for researchers interested in furthering their studies in plant protein secretion."
9781493972623 1493972626
10.1007/978-1-4939-7262-3 doi
Plant proteins--Secretion--Laboratory manuals.
Plant Proteins--metabolism
Plants--metabolism
Secretory Vesicles--metabolism
Golgi Apparatus--metabolism
Secretory Pathway
Protéines végétales--Sécrétion--Manuels de laboratoire.
Botany & plant sciences.
Science--Life Sciences--Botany.
Botany
Life sciences
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QK898.P8
572/.62
Includes bibliographical references and index.
An overview of protein secretion in yeast and animal cells / An overview of protein secretion in plant cells / Bioinformatics analysis of protein secretion in plants / Proteomic analysis of secreted proteins from cell microenvironment / Using homology modeling to understand the structural basis of specific interaction of a plant-specific AtSar1a-AtSec23a pair involved in protein ER export / Analysis of Golgi-mediated protein traffic in plant cells / Analysis of membrane protein topology in the plant secretory pathway / Semiautomatic segmentation of plant Golgi stacks in electron tomograms using 3dmod / 3D printing of plant Golgi stacks from their electron tomographic models / Transient expression of chimeric fluorescent reporter proteins in pollen tubes to study protein polar secretion and dynamics / Analysis of actin-based intracellular trafficking in pollen tubes / Analysis of phragmoplast kinetics during plant cytokinesis / Immunofluorescence analysis of membrane-associated proteins for calthrin-mediated endocytosis in plant root cells / In vivo interaction studies by measuring Förster resonance energy transfer through fluorescence lifetime imaging microscopy (FRET/FLIM) / Analysis of nano-body-epitope interactions in living cells via quantitative protein transport assays / A secretion system for cargo protein identification of vacuolar sorting receptors / Identifying novel regulators of vacuolar trafficking by combining fluorescence imaging-based forward genetic screening and in vitro pollen germination / Measuring plan protein secretion / Transient secretory enzyme expression in leaf protoplasts to characterize SNARE functional classes in conventional and unconventional secretion / The organelle pH estimate and measurement in plant secratory pathway / Analysis of exocyst-positive organelle (EXPO)-mediated unconventional protein secretion (UPS) in plant cells / Isolation of the plant exocyst complex / Using microscopy tools to visualize autophagosomal structures in plant cells / Analysis of plant autophagy / Yusong Guo, Feng Yang, Xiao Tang -- Kin Pau Chung, Yonglun Zeng -- Liyuan Chen -- Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian -- Yap-Shing Nim, Shuangli Sun, Kam-Bo Wong -- Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao -- Jinya Guo, Yansong Miao, Yi Cai -- Keith Ka Ki Mai, Byung-Ho Kang -- Keith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang -- Guitao Zhong, Ronghe Liu, Menglong Zhuang, Hao Wang -- Yuxiang Jiang, Meng Zhang, Shanjin Huang -- Pantelis Livanos, Mayank Chugh, Sabine Müller -- Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, Jianwei Pan -- Florian Fässler, Peter Pimpl -- Simone Früholz and Peter Pimpl -- Jinbo Shen -- Qiang-Nan Feng, Yan Zhang -- Emily R. Larson -- 19. Di Sansebastiano Gian Pietro, Barozzi Fabrizio -- Jinbo Shen -- Yu Ding, Juan Wang -- King Pong Leung, Wilson Chun Yu Lau -- Weili Lin, Xiaohong Zhuang -- Liang Chen, Faqiang Li, Shi Xiao. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 20. 21. 22. 23. 24.
This volume aims to provide an update on recent developments in protein secretion studies in plants versus yeast and mammalian systems. This book also discusses case studies that analyze the use of plant protein secretion using various tools and systems. The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Protein Secretion: Methods and Protocols is a valuable resource for researchers interested in furthering their studies in plant protein secretion."
9781493972623 1493972626
10.1007/978-1-4939-7262-3 doi
Plant proteins--Secretion--Laboratory manuals.
Plant Proteins--metabolism
Plants--metabolism
Secretory Vesicles--metabolism
Golgi Apparatus--metabolism
Secretory Pathway
Protéines végétales--Sécrétion--Manuels de laboratoire.
Botany & plant sciences.
Science--Life Sciences--Botany.
Botany
Life sciences
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QK898.P8
572/.62