The plant endoplasmic reticulum : methods and protocols /
The plant endoplasmic reticulum : methods and protocols /
edited by Chris Hawes and Verena Kriechbaumer.
- 1 online resource (xiv, 265 pages) : illustrations (some color)
- Methods in molecular biology, 1691 1064-3745 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1691. .
Includes bibliographical references and index.
Labeling the ER for light and fluorescence microscopy / 3D electron microscopy of the ER / Characterization of proteins localized to plant ER-PM contact sites / Preparation and imaging of specialized ER using super-resolution and TEM techniques / Quantitation of ER structure and function / Long-term imaging of endoplasmic reticulum morphology in embryos during seed germination / Dancing with the stars : using image analysis to study the choreography of the endoplasmic reticulum and its partners and of movement within its tubules / Preparation of highly enriched ER membranes using free-flow electrophoresis / ER microsome preparation in Arabidopsis thaliana / ER membrane lipid composition and metabolism : lipidomic analysis / 2in1 vectors improve in planta BiFC and FRET analyses / Metabolons on the plant ER / Using optical tweezers combined with total internal reflection microscopy to study interactions between the ER and golgi in plant cells / Protein biosynthesis and maturation in the ER / ER membrane protein interactions using the split-ubiquitin system (SUS) / Analysis of protein glycosylation in the ER / Unfolded protein response / Unfolded protein response in Arabidopsis / Fluorescence imaging of autophagy-mediated ER-to-vacuole trafficking in plants / Imaging the ER and endomembrane system in cereal endosperm / Chris Hawes, Pengwei Wang, and Verena Kriechbaumer -- Maike Kittelmann -- Pengwei Wang, Chris Hawes, Christine Richardson, and Patrick J. Hussey -- Karen Bell, Karl Oparka, and Kirsten Knox -- Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. Müller, and Andreas J. Meyer -- Natasha Dzimitrowicz, Emily Breeze, and Lorenzo Frigerio -- Lawrence R. Griffing -- Harriet T. Parsons -- Verena Kriechbaumer -- Laetitia Fouillen, Lilly Maneta-Peyret, and Patrick Moreau -- Dietmar G. Mehlhorn, Niklas Wallmeroth, Kenneth W. Berendzen, and Christopher Grefen -- Verena Kriechbaumer and Stanley W. Botchway -- Imogen Sparkes, Rhiannon R. White, Benjamin Coles, Stanley W. Botchway, and Andy Ward -- Emanuela Pedrazzini and Alessandro Vitale -- Lisa Yasmin Asseck, Niklas Wallmeroth, and Christopher Grefen -- Jennifer Schoberer, Yun-Ji Shin, Ulrike Vavra, Christiane Veit, and Richard Strasser -- Kazuki Tabara, Yuji Iwata, and Nozomu Koizumi -- Cristina Ruberti and Federica Brandizzi -- Hadas Peled-Zehavi and Gad Galili -- Verena Ibl, Jenny Peters, Eva Stöger, and Elsa Arcalı́s.
This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field.
9781493973897 1493973894 1493973886 9781493973880
Endoplasmic reticulum--Laboratory manuals.
Plant genetics--Laboratory manuals.
Endoplasmic reticulum.
Endoplasmic Reticulum
Plants
Réticulum endoplasmique--Manuels de laboratoire.
Génétique végétale--Manuels de laboratoire.
Réticulum endoplasmique.
Plantes.
vegetation.
Endoplasmic reticulum
Plant genetics
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QH603.E6
571.6/5
W1 / ME9616J v.1691 2018 QK 725
Includes bibliographical references and index.
Labeling the ER for light and fluorescence microscopy / 3D electron microscopy of the ER / Characterization of proteins localized to plant ER-PM contact sites / Preparation and imaging of specialized ER using super-resolution and TEM techniques / Quantitation of ER structure and function / Long-term imaging of endoplasmic reticulum morphology in embryos during seed germination / Dancing with the stars : using image analysis to study the choreography of the endoplasmic reticulum and its partners and of movement within its tubules / Preparation of highly enriched ER membranes using free-flow electrophoresis / ER microsome preparation in Arabidopsis thaliana / ER membrane lipid composition and metabolism : lipidomic analysis / 2in1 vectors improve in planta BiFC and FRET analyses / Metabolons on the plant ER / Using optical tweezers combined with total internal reflection microscopy to study interactions between the ER and golgi in plant cells / Protein biosynthesis and maturation in the ER / ER membrane protein interactions using the split-ubiquitin system (SUS) / Analysis of protein glycosylation in the ER / Unfolded protein response / Unfolded protein response in Arabidopsis / Fluorescence imaging of autophagy-mediated ER-to-vacuole trafficking in plants / Imaging the ER and endomembrane system in cereal endosperm / Chris Hawes, Pengwei Wang, and Verena Kriechbaumer -- Maike Kittelmann -- Pengwei Wang, Chris Hawes, Christine Richardson, and Patrick J. Hussey -- Karen Bell, Karl Oparka, and Kirsten Knox -- Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. Müller, and Andreas J. Meyer -- Natasha Dzimitrowicz, Emily Breeze, and Lorenzo Frigerio -- Lawrence R. Griffing -- Harriet T. Parsons -- Verena Kriechbaumer -- Laetitia Fouillen, Lilly Maneta-Peyret, and Patrick Moreau -- Dietmar G. Mehlhorn, Niklas Wallmeroth, Kenneth W. Berendzen, and Christopher Grefen -- Verena Kriechbaumer and Stanley W. Botchway -- Imogen Sparkes, Rhiannon R. White, Benjamin Coles, Stanley W. Botchway, and Andy Ward -- Emanuela Pedrazzini and Alessandro Vitale -- Lisa Yasmin Asseck, Niklas Wallmeroth, and Christopher Grefen -- Jennifer Schoberer, Yun-Ji Shin, Ulrike Vavra, Christiane Veit, and Richard Strasser -- Kazuki Tabara, Yuji Iwata, and Nozomu Koizumi -- Cristina Ruberti and Federica Brandizzi -- Hadas Peled-Zehavi and Gad Galili -- Verena Ibl, Jenny Peters, Eva Stöger, and Elsa Arcalı́s.
This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field.
9781493973897 1493973894 1493973886 9781493973880
Endoplasmic reticulum--Laboratory manuals.
Plant genetics--Laboratory manuals.
Endoplasmic reticulum.
Endoplasmic Reticulum
Plants
Réticulum endoplasmique--Manuels de laboratoire.
Génétique végétale--Manuels de laboratoire.
Réticulum endoplasmique.
Plantes.
vegetation.
Endoplasmic reticulum
Plant genetics
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QH603.E6
571.6/5
W1 / ME9616J v.1691 2018 QK 725