Autophagy : methods and protocols /
Autophagy : methods and protocols /
edited by Nicholas Ktistakis and Oliver Florey.
- 1 online resource (xvii, 741 pages) : illustrations (some color)
- Methods in molecular biology, volume 1880 1940-6029 ; .
- Methods in molecular biology (Clifton, N.J.) ; v. 1880. .
Includes bibliographical references and index.
Recombinant expression, purification, and assembly of p62 filaments / Structural studies of autophagy-related proteins / Structural studies of mammalian autophagy lipidation complex / Structural studies of selective autophagy in yeast / Biophysical studies of LC3 family proteins / Reconstituting autophagy initiation from purified components / Cell-free reconstitution of autophagic membrane formation / Use of peptide arrays for identification and characterization of LIR motifs / Studying autophagic lysosome reformation in cells and by an in vitro reconstitution System / Formation of autophagosomes coincides with relaxation of membrane curvature / Studies of receptor-Atg8 interactions during selective autophagy / Correlative light and electron microscopy of autophagosomes / Improved electron microscopy fixation methods for tracking autophagy-associated membranes in cultured mammalian cells / Three-color simultaneous live imaging of autophagy-related structures / Correlative live-cell imaging and super-resolution microscopy of autophagy / Methods for imaging autophagosome dynamics in primary neurons / Imaging autophagy in hiPSC-derived midbrain dopaminergic neuronal cultures for parkinson's disease research / Correlative light and electron microscopy to analyze LC3 proteins in Caenorhabditis elegans embryo / Imaging noncanonical autophagy and LC3-associated phagocytosis in cultured cells / Measurement of bulk autophagy by a cargo sequestration assay / Methods to detect loss of lysosomal membrane integrity / Monitoring of autophagy and cell volume regulation in kidney epithelial cells in response to fluid shear stress / Identification and regulation of multimeric protein complexes in autophagy via SILAC-based mass spectrometry approaches / Identification and validation of novel autophagy regulators using an endogenous readout siGENOME screen / Autophagy pathway mapping to elucidate the function of novel autophagy regulators identified by high-throughput screening / In vitro screening platforms for identifying autophagy modulators in mammalian cells / Automated detection of autophagy response using single cell-based microscopy assays / Methods for the study of entotic cell death / MHC class I internalization via autophagy proteins / Analysis of autophagy for liver pathogenesis / Autophagy in 3D in vitro and ex vivo cancer models / Autophagy in platelets / Methods to image macroautophagy in the brain in vivo / Measuring nonselective and selective autophagy in the liver / Measuring autophagy in pancreatitis / Characterization of the "Autophagic Flux" in prostate cancer tissue biopsies by LC3A/LAMP2a immunofluorescence and confocal microscopy / Methods to determine the role of autophagy proteins in C. elegans aging / Investigating non-selective autophagy in Drosophila / Imaging the dynamics of mitophagy in live cells / Triggering mitophagy with photosensitizers / Investigating mitophagy and mitochondrial morphology in vivo using mito-QC : a comprehensive guide / Assays to monitor mitophagy in Drosophila / Mitophagy dynamics in Caenorhabditis elegans / Methods for studying mitophagy in yeast / Measuring antibacterial autophagy / Quantitative phosphoproteomics of selective autophagy receptors / Analysis of chaperone-mediated autophagy / Interactive autophagy : monitoring a novel form of selective autophagy by macroscopic observations / Abul K. Tarafder, Audrey Guesdon, Tanja Kuhm, and Carsten Sachse -- Melanie Schwarten, Oliver H. Weiergr€aber, Dusˇan Petrovic´,Birgit Strodel, and Dieter Willbold -- Kazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, and Takanori Otomo -- Akinori Yamasaki, Yasunori Watanabe, and Nobuo N. Noda -- Javier H. Herva s, Zurine Anton, and Alicia Alonso -- Peter Mayrhofer and Thomas Wollert -- Min Zhang and Liang Ge -- Mads Skytte Rasmussen, Asa Birna Birgisdottir, and Terje Johansen -- Yang Chen, Qian Peter Su, and Li Yu -- Jaime Agudo-Canalejo and Roland L. Knorr -- Christine Abert and Sascha Martens -- Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, and Eeva-Liisa Eskelinen -- Ritsuko Arai and Satoshi Waguri -- Hiroyuki Ueda, Ouin Kunitaki, and Maho Hamasaki -- Eleftherios Karanasios -- Audrey Dong, Vineet Vinay Kulkarni, and Sandra Maday -- Petros Stathakos, Natalia Jimenez-Moreno, Lucy Crompton, Paul Nistor, Maeve A. Caldwell, and Jon D. Lane -- Ce´line Largeau and Renaud Legouis -- Elise Jacquin, Katherine Fletcher, and Oliver Florey -- Nikolai Engedal, Morten Luhr, Paula Szalai, and Per O. Seglen -- Sonja Aits -- Maria M. Lazari, Idil Orhon, Patrice Codogno, and Nicolas Dupont -- Ste´phanie Kaeser-Pebernard, Britta Diedrich, and Jorn Dengjel -- Maria New, Tim Van Acker, Ming Jiang, Rebecca Saunders, Jaclyn S. Long, Jun-Ichi Sakamaki, Kevin M. Ryan, Michael Howell, and Sharon A. Tooze -- Martina Wirth and Sharon A. Tooze -- Elena Seranova, Carl Ward, Miruna Chipara, Tatiana R. Rosenstock, and Sovan Sarkar -- Amelie J. Mueller and Tassula Proikas-Cezanne -- Jens C. Hamann, Sung Eun Kim, and Michael Overholtzer -- Monica Loi, Laure-Anne Ligeon, and Christian Mu¨nz -- Nazmul Huda, Hui Zou, Shengmin Yan, Bilon Khambu, and Xiao-Ming Yin -- Carlo Follo, Dario Barbone, William G. Richards,Raphael Bueno, and V. Courtney Broaddus -- Meenakshi Banerjee, Yunjie Huang, Madhu M. Ouseph, Smita Joshi, Irina Pokrovskaya, Brian Storrie, Jinchao Zhang, Sidney W. Whiteheart, and Qing Jun Wang -- Xigui Chen, Kanoh Kondo, and Hitoshi Okazawa -- Takashi Ueno and Masaaki Komatsu -- Alejandro Ropolo, Daniel Grasso, and Maria I. Vaccaro -- D. Kalamida, A. Giatromanolaki, and M. I. Koukourakis -- Sivan Henis-Korenblit and Alicia Mele´ndez -- Szabolcs Taka´ ts, Sarolta Toth, Gyo˝zo˝ Szenci, and Ga´ bor Juha´ sz -- Andrew S. Moore and Erika L. F. Holzbaur -- Cheng-Wei Hsieh and Wei Yuan Yang -- Thomas G. McWilliams and Ian G. Ganley -- Panagiotis Tsapras, Anne-Claire Jacomin, and Ioannis P. Nezis -- Konstantinos Palikaras, Eirini Lionaki, and Nektarios Tavernarakis -- Panagiota Kolitsida and Hagai Abeliovich -- Keith B. Boyle and Felix Randow -- Thomas Juretschke, Petra Beli, and Ivan Dikic -- Y. R. Juste and A. M. Cuervo -- Jana Petri and Roland L. Knorr.
This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
9781493988730 1493988735
com.springer.onix.9781493988730 Springer Nature
GBB935134 bnb
019219000 Uk
Cell death--Laboratory manuals.
Apoptosis--Laboratory manuals.
Autophagy
Cellules--Mort--Manuels de laboratoire.
Apoptose--Manuels de laboratoire.
Autophagie (Cytologie)
Apoptosis
Cell death
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QH671
571.9/36
Includes bibliographical references and index.
Recombinant expression, purification, and assembly of p62 filaments / Structural studies of autophagy-related proteins / Structural studies of mammalian autophagy lipidation complex / Structural studies of selective autophagy in yeast / Biophysical studies of LC3 family proteins / Reconstituting autophagy initiation from purified components / Cell-free reconstitution of autophagic membrane formation / Use of peptide arrays for identification and characterization of LIR motifs / Studying autophagic lysosome reformation in cells and by an in vitro reconstitution System / Formation of autophagosomes coincides with relaxation of membrane curvature / Studies of receptor-Atg8 interactions during selective autophagy / Correlative light and electron microscopy of autophagosomes / Improved electron microscopy fixation methods for tracking autophagy-associated membranes in cultured mammalian cells / Three-color simultaneous live imaging of autophagy-related structures / Correlative live-cell imaging and super-resolution microscopy of autophagy / Methods for imaging autophagosome dynamics in primary neurons / Imaging autophagy in hiPSC-derived midbrain dopaminergic neuronal cultures for parkinson's disease research / Correlative light and electron microscopy to analyze LC3 proteins in Caenorhabditis elegans embryo / Imaging noncanonical autophagy and LC3-associated phagocytosis in cultured cells / Measurement of bulk autophagy by a cargo sequestration assay / Methods to detect loss of lysosomal membrane integrity / Monitoring of autophagy and cell volume regulation in kidney epithelial cells in response to fluid shear stress / Identification and regulation of multimeric protein complexes in autophagy via SILAC-based mass spectrometry approaches / Identification and validation of novel autophagy regulators using an endogenous readout siGENOME screen / Autophagy pathway mapping to elucidate the function of novel autophagy regulators identified by high-throughput screening / In vitro screening platforms for identifying autophagy modulators in mammalian cells / Automated detection of autophagy response using single cell-based microscopy assays / Methods for the study of entotic cell death / MHC class I internalization via autophagy proteins / Analysis of autophagy for liver pathogenesis / Autophagy in 3D in vitro and ex vivo cancer models / Autophagy in platelets / Methods to image macroautophagy in the brain in vivo / Measuring nonselective and selective autophagy in the liver / Measuring autophagy in pancreatitis / Characterization of the "Autophagic Flux" in prostate cancer tissue biopsies by LC3A/LAMP2a immunofluorescence and confocal microscopy / Methods to determine the role of autophagy proteins in C. elegans aging / Investigating non-selective autophagy in Drosophila / Imaging the dynamics of mitophagy in live cells / Triggering mitophagy with photosensitizers / Investigating mitophagy and mitochondrial morphology in vivo using mito-QC : a comprehensive guide / Assays to monitor mitophagy in Drosophila / Mitophagy dynamics in Caenorhabditis elegans / Methods for studying mitophagy in yeast / Measuring antibacterial autophagy / Quantitative phosphoproteomics of selective autophagy receptors / Analysis of chaperone-mediated autophagy / Interactive autophagy : monitoring a novel form of selective autophagy by macroscopic observations / Abul K. Tarafder, Audrey Guesdon, Tanja Kuhm, and Carsten Sachse -- Melanie Schwarten, Oliver H. Weiergr€aber, Dusˇan Petrovic´,Birgit Strodel, and Dieter Willbold -- Kazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, and Takanori Otomo -- Akinori Yamasaki, Yasunori Watanabe, and Nobuo N. Noda -- Javier H. Herva s, Zurine Anton, and Alicia Alonso -- Peter Mayrhofer and Thomas Wollert -- Min Zhang and Liang Ge -- Mads Skytte Rasmussen, Asa Birna Birgisdottir, and Terje Johansen -- Yang Chen, Qian Peter Su, and Li Yu -- Jaime Agudo-Canalejo and Roland L. Knorr -- Christine Abert and Sascha Martens -- Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, and Eeva-Liisa Eskelinen -- Ritsuko Arai and Satoshi Waguri -- Hiroyuki Ueda, Ouin Kunitaki, and Maho Hamasaki -- Eleftherios Karanasios -- Audrey Dong, Vineet Vinay Kulkarni, and Sandra Maday -- Petros Stathakos, Natalia Jimenez-Moreno, Lucy Crompton, Paul Nistor, Maeve A. Caldwell, and Jon D. Lane -- Ce´line Largeau and Renaud Legouis -- Elise Jacquin, Katherine Fletcher, and Oliver Florey -- Nikolai Engedal, Morten Luhr, Paula Szalai, and Per O. Seglen -- Sonja Aits -- Maria M. Lazari, Idil Orhon, Patrice Codogno, and Nicolas Dupont -- Ste´phanie Kaeser-Pebernard, Britta Diedrich, and Jorn Dengjel -- Maria New, Tim Van Acker, Ming Jiang, Rebecca Saunders, Jaclyn S. Long, Jun-Ichi Sakamaki, Kevin M. Ryan, Michael Howell, and Sharon A. Tooze -- Martina Wirth and Sharon A. Tooze -- Elena Seranova, Carl Ward, Miruna Chipara, Tatiana R. Rosenstock, and Sovan Sarkar -- Amelie J. Mueller and Tassula Proikas-Cezanne -- Jens C. Hamann, Sung Eun Kim, and Michael Overholtzer -- Monica Loi, Laure-Anne Ligeon, and Christian Mu¨nz -- Nazmul Huda, Hui Zou, Shengmin Yan, Bilon Khambu, and Xiao-Ming Yin -- Carlo Follo, Dario Barbone, William G. Richards,Raphael Bueno, and V. Courtney Broaddus -- Meenakshi Banerjee, Yunjie Huang, Madhu M. Ouseph, Smita Joshi, Irina Pokrovskaya, Brian Storrie, Jinchao Zhang, Sidney W. Whiteheart, and Qing Jun Wang -- Xigui Chen, Kanoh Kondo, and Hitoshi Okazawa -- Takashi Ueno and Masaaki Komatsu -- Alejandro Ropolo, Daniel Grasso, and Maria I. Vaccaro -- D. Kalamida, A. Giatromanolaki, and M. I. Koukourakis -- Sivan Henis-Korenblit and Alicia Mele´ndez -- Szabolcs Taka´ ts, Sarolta Toth, Gyo˝zo˝ Szenci, and Ga´ bor Juha´ sz -- Andrew S. Moore and Erika L. F. Holzbaur -- Cheng-Wei Hsieh and Wei Yuan Yang -- Thomas G. McWilliams and Ian G. Ganley -- Panagiotis Tsapras, Anne-Claire Jacomin, and Ioannis P. Nezis -- Konstantinos Palikaras, Eirini Lionaki, and Nektarios Tavernarakis -- Panagiota Kolitsida and Hagai Abeliovich -- Keith B. Boyle and Felix Randow -- Thomas Juretschke, Petra Beli, and Ivan Dikic -- Y. R. Juste and A. M. Cuervo -- Jana Petri and Roland L. Knorr.
This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
9781493988730 1493988735
com.springer.onix.9781493988730 Springer Nature
GBB935134 bnb
019219000 Uk
Cell death--Laboratory manuals.
Apoptosis--Laboratory manuals.
Autophagy
Cellules--Mort--Manuels de laboratoire.
Apoptose--Manuels de laboratoire.
Autophagie (Cytologie)
Apoptosis
Cell death
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
QH671
571.9/36