Caenorhabditis elegans : modern biological analysis of an organism /
Caenorhabditis elegans : modern biological analysis of an organism /
edited by Henry F. Epstein and Diane C. Shakes.
- 1 online resource (xxi, 659 pages) : illustrations
- Methods in cell biology, v. 48 0091-679X ; .
- Methods in cell biology ; v. 48. .
"Prepared under the auspices of the American Society for Cell Biology."
Includes bibliographical references and index.
pt. 1. Genetic and culture methods. Basic culture methods / James A. Lewis, John T. Fleming -- Mutagenesis / Philip Anderson -- Reverse genetics : from gene sequence to mutant worm / Ronald H.A. Plasterk -- Genetic mapping with polymorphic sequence-tagged sites / Benjamin D. Williams -- Genetic dissection of developmental pathways / Linda S. Huang, Paul W. Sternberg -- Mosaic analysis / Robert K. Herman -- Genetic balancers / Mark L. Edgley [and others] -- pt 2. Neurobiology. Genetic pharmacology : interactions between drugs and gene products in Caenorhabditis elegans / James B. Rand, Carl D. Johnson -- Methods of studying behavioral plasticity in Caenorhabditis elegans / Timothy N. Gannon, Catharine H. Rankin -- Laser killing of cells in Caenorhabditis elegans / Cornelia I. Bargmann, Leon Avery -- Electrophysiological methods / Leon Avery, David Raizen, Shawn Lockery -- pt. 3. Cell biology and molecular biology. Cell biology of nematode sperm / Steven W. L'Hernault, Thomas M. Roberts -- Blastomere culture and analysis / Lois G. Edgar -- Whole-mount in situ hybridization for the detection of RNA in Caenorhabditis elegans embryos / Geraldine Seydoux, Andrew Fire -- Fluorescence in situ hybridization for the detection of DNA and RNA / Donna G. Albertson, Rita M. Fishpool, Philip S. Birchall -- Immunofluorescence microscopy / David M. Miller, Diane C. Shakes -- Electron microscopy and three-dimensional image reconstruction / David H. Hall. Proteins and protein assemblies / Henry F. Epstein, Feizhou Liu -- DNA transformation / Craig Mello, Andrew Fire -- Transcription and translation ; Techniques for analyzing transcription and translation / Michael Krause -- pt. 4. Genomics and informatics. The physical map of the Caenorhabditis elegans genome / Alan Coulson [and others] -- Genomic DNA sequencing methods / Anthony Favello, LaDeana Hillier, Richard K. Wilson -- Large-scale complementary DNA sequencing methods / Lucinda L. Fulton, LaDeana Hillier, Richard K. Wilson -- ACeDB and Macace / Frank H. Eeckman, Richard Durbin -- The Worm Community System, release 2.0 (WCSr2) / Laura M. Shoman [and others] -- Appendix A. Additional information and resources -- Appendix B. Caenorhabditis Genetics Center, laboratory designations -- Appendix C. Gene names and descriptions.
The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr.
9780080859460 0080859461 0125641494 9780125641494
(WaSeSS)ssj0000116712
GB9586629 bnb
9517120 DNLM 006304713 Uk
Caenorhabditis elegans.
Genetics--Technique.
Caenorhabditis elegans--genetics
Genetic Techniques
Caenorhabditis elegans
Cænorhabditis elegans.
Génétique--Technique.
SCIENCE--Life Sciences--Zoology--General.
Genetics--Technique
Caenorhabditis elegans
Caenorhabditis elegans
Molekularbiologie
Cytologie
Methode
Genanalyse
Genetik
Neurobiologie
Genom
Caenorhabditis elegans.
Celbiologie.
Genanalyse.
Nematodes
QH585 / .M47eb vol. 48
595.182
442 / P92 v.48
W1 / ME9615D v.48 1995 QX 203 / C127 1995
"Prepared under the auspices of the American Society for Cell Biology."
Includes bibliographical references and index.
pt. 1. Genetic and culture methods. Basic culture methods / James A. Lewis, John T. Fleming -- Mutagenesis / Philip Anderson -- Reverse genetics : from gene sequence to mutant worm / Ronald H.A. Plasterk -- Genetic mapping with polymorphic sequence-tagged sites / Benjamin D. Williams -- Genetic dissection of developmental pathways / Linda S. Huang, Paul W. Sternberg -- Mosaic analysis / Robert K. Herman -- Genetic balancers / Mark L. Edgley [and others] -- pt 2. Neurobiology. Genetic pharmacology : interactions between drugs and gene products in Caenorhabditis elegans / James B. Rand, Carl D. Johnson -- Methods of studying behavioral plasticity in Caenorhabditis elegans / Timothy N. Gannon, Catharine H. Rankin -- Laser killing of cells in Caenorhabditis elegans / Cornelia I. Bargmann, Leon Avery -- Electrophysiological methods / Leon Avery, David Raizen, Shawn Lockery -- pt. 3. Cell biology and molecular biology. Cell biology of nematode sperm / Steven W. L'Hernault, Thomas M. Roberts -- Blastomere culture and analysis / Lois G. Edgar -- Whole-mount in situ hybridization for the detection of RNA in Caenorhabditis elegans embryos / Geraldine Seydoux, Andrew Fire -- Fluorescence in situ hybridization for the detection of DNA and RNA / Donna G. Albertson, Rita M. Fishpool, Philip S. Birchall -- Immunofluorescence microscopy / David M. Miller, Diane C. Shakes -- Electron microscopy and three-dimensional image reconstruction / David H. Hall. Proteins and protein assemblies / Henry F. Epstein, Feizhou Liu -- DNA transformation / Craig Mello, Andrew Fire -- Transcription and translation ; Techniques for analyzing transcription and translation / Michael Krause -- pt. 4. Genomics and informatics. The physical map of the Caenorhabditis elegans genome / Alan Coulson [and others] -- Genomic DNA sequencing methods / Anthony Favello, LaDeana Hillier, Richard K. Wilson -- Large-scale complementary DNA sequencing methods / Lucinda L. Fulton, LaDeana Hillier, Richard K. Wilson -- ACeDB and Macace / Frank H. Eeckman, Richard Durbin -- The Worm Community System, release 2.0 (WCSr2) / Laura M. Shoman [and others] -- Appendix A. Additional information and resources -- Appendix B. Caenorhabditis Genetics Center, laboratory designations -- Appendix C. Gene names and descriptions.
The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr.
9780080859460 0080859461 0125641494 9780125641494
(WaSeSS)ssj0000116712
GB9586629 bnb
9517120 DNLM 006304713 Uk
Caenorhabditis elegans.
Genetics--Technique.
Caenorhabditis elegans--genetics
Genetic Techniques
Caenorhabditis elegans
Cænorhabditis elegans.
Génétique--Technique.
SCIENCE--Life Sciences--Zoology--General.
Genetics--Technique
Caenorhabditis elegans
Caenorhabditis elegans
Molekularbiologie
Cytologie
Methode
Genanalyse
Genetik
Neurobiologie
Genom
Caenorhabditis elegans.
Celbiologie.
Genanalyse.
Nematodes
QH585 / .M47eb vol. 48
595.182
442 / P92 v.48
W1 / ME9615D v.48 1995 QX 203 / C127 1995