Guide to protein purification /
Guide to protein purification /
edited by Murray P. Deutscher.
- San Diego : Academic Press, ©1990.
- 1 online resource (xxix, 894 pages) : illustrations
- Methods in enzymology, v. 182 0076-6879 ; .
- Methods in enzymology ; v. 182. .
Includes bibliographical references and indexes.
Why purify enzymes / Arthur Kornberg -- Strategies and considerations for protein purifications / Stuart Linn -- Setting up a laboratory / Murray P. Deutscher -- Buffers: Principles and practice / Vincent S. Stoll, John S. Blanchard -- Measurement of enzyme activity / Edward F. Rossomando -- Quantitation of protein / Christa M. Stoscheck -- Concentration of proteins and removal of solutes / Thomas Pohl -- Maintaining protein stability / Murray P. Deutscher -- Overproduction of proteins in Escherichia coli: Vectors, hosts, and strategies / Asis Das -- Overexpression of proteins in eukaryotes / Margaret K. Bradley -- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland, Brendan Kenny, Boris Steipe, Andreas Plückthun -- Preparation of extracts from prokaryotes / Millard Cull, Charles S. McHenry -- Preparation of extracts from yeast / S. Michal Jazwinski -- Preparation of Extracts from Plants / Peter Gegenheimer -- Preparation of extracts from higher eukaryotes / John David Dignam -- Isolation of subcellular organelles / Brian Storrie, Edward A Madden -- Preparation of membrane fractions / Juris Ozols -- Detergents: An overview / Judith M. Neugebauer -- Solubilization of native membrane proteins / Leonard M. Hjelmeland -- Solubilization of protein aggregates / Fiona A.O. Marston, Donna L. Hartley -- Removal of detergents from membrane proteins / Leonard M. Hjelmeland -- Precipitation techniques / Sasha Englard, Sam Seifter -- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham -- Ion-exchange chromatography / Edward F. Rossomando -- Gel filtration / Earle Stellwagen -- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- Hydrophobic chromatography / Robert M. Kennedy -- Chromatography on immobilized reactive dyes / Earle Stellwagen -- Affinity chromatography: General methods / Steven Ostrove -- Affinity chromatography: Specialized techniques / Steven Ostrove, Shelly Weiss -- Chromatofocusing / Lallan Giri -- High-performance liquid chromatography: Effective protein purification by various chromatographic modes / Roman M. Chicz, Fred E. Regnier -- One-dimensional gel electrophoresis / David E. Garfin -- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura, Therese M. Timmons -- Isoelectric focusing / David E. Garfin -- Gel-staining techniques / Carl R. Merril -- Elution of protein from gels / Michael G. Harrington -- Purification of membrane proteins / Thomas C. Thomas, Mark G. McNamee -- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg, Stephen C. Francesconi, Chris Civalier, Scott S. Walker -- Purification of glycoproteins / Craig Gerard -- Purification of multienzyme complexes / Paul A. Srere, Christopher K. Mathews -- Determination of purity / David G. Rhodes, Thomas M. Laue -- Determination of size, molecular weight, and presence of subunits / Thomas M. Laue, David G. Rhodes -- Amino acid analysis / Juris Ozols -- Limited N-terminal sequence analysis / Paul Matsudaira -- Peptide mapping / Ralph C. Judd -- Analysis for protein modifications and nonprotein cofactors / Sam Seifter, Sasha Englard -- Protein crystallization / David Ollis, Stephen White -- Preparation of polyclonal antibodies / Bonnie S. Dunbar, Eric D. Schwoebel -- Preparation of monoclonal antibodies / Bonnie S. Dunbar, Sheri M. Skinner -- Protein blotting and immunodetection / Therese M. Timmons, Bonnie S. Dunbar -- Immunoprecipitation of proteins / Gary L. Firestone, Sandra D. Winguth -- Immunoassays / Charles W. Parker -- Radiolabeling of proteins / Charles W. Parker -- Using purified protein to clone its gene / John M. Wozney -- Computer analysis of protein structure / Patrick Argos -- Rethinking your purification procedure / Murray P. Deutscher -- The private life of DNA polymerase I / Arthur Kornberg -- Purification and crystallization of transaldolase from Candida utilis / B.L. Horecker, O. Tsolas -- Discovery of glutamine synthetase cascade / Earl R. Stadtman -- Discovery, resolution, purification, and function of elongation factors / Kivie Moldave.
Use copy
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for protein and enzyme purification * Personal perspectives on enzyme purification by eminent researchers Among the Topics Covered * General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
Electronic reproduction.
[Place of publication not identified] :
HathiTrust Digital Library,
2011.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
http://purl.oclc.org/DLF/benchrepro0212
English.
0121820831 9780121820831
(WaSeSS)ssj0000492525
Proteins--Purification.
Proteins.
Proteins--isolation & purification
Protéines--Purification.
Protéines.
protein.
Proteins--Purification
Proteine
Reinigung
Reinigungsverfahren
Eiwitten.
Enzymen.
Zuiveringstechnieken.
Laboratoriumonderzoek.
Proteine Reinigungsverfahren Proteine Reinigung
Electronic books.
QP601 / .M49 v.182 QP551
574.19296
577.15 N0.182
QP601.M49 / v.182
W1 [QU 55] / ME9615K v.182 1990 QU 55
Includes bibliographical references and indexes.
Why purify enzymes / Arthur Kornberg -- Strategies and considerations for protein purifications / Stuart Linn -- Setting up a laboratory / Murray P. Deutscher -- Buffers: Principles and practice / Vincent S. Stoll, John S. Blanchard -- Measurement of enzyme activity / Edward F. Rossomando -- Quantitation of protein / Christa M. Stoscheck -- Concentration of proteins and removal of solutes / Thomas Pohl -- Maintaining protein stability / Murray P. Deutscher -- Overproduction of proteins in Escherichia coli: Vectors, hosts, and strategies / Asis Das -- Overexpression of proteins in eukaryotes / Margaret K. Bradley -- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland, Brendan Kenny, Boris Steipe, Andreas Plückthun -- Preparation of extracts from prokaryotes / Millard Cull, Charles S. McHenry -- Preparation of extracts from yeast / S. Michal Jazwinski -- Preparation of Extracts from Plants / Peter Gegenheimer -- Preparation of extracts from higher eukaryotes / John David Dignam -- Isolation of subcellular organelles / Brian Storrie, Edward A Madden -- Preparation of membrane fractions / Juris Ozols -- Detergents: An overview / Judith M. Neugebauer -- Solubilization of native membrane proteins / Leonard M. Hjelmeland -- Solubilization of protein aggregates / Fiona A.O. Marston, Donna L. Hartley -- Removal of detergents from membrane proteins / Leonard M. Hjelmeland -- Precipitation techniques / Sasha Englard, Sam Seifter -- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham -- Ion-exchange chromatography / Edward F. Rossomando -- Gel filtration / Earle Stellwagen -- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- Hydrophobic chromatography / Robert M. Kennedy -- Chromatography on immobilized reactive dyes / Earle Stellwagen -- Affinity chromatography: General methods / Steven Ostrove -- Affinity chromatography: Specialized techniques / Steven Ostrove, Shelly Weiss -- Chromatofocusing / Lallan Giri -- High-performance liquid chromatography: Effective protein purification by various chromatographic modes / Roman M. Chicz, Fred E. Regnier -- One-dimensional gel electrophoresis / David E. Garfin -- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura, Therese M. Timmons -- Isoelectric focusing / David E. Garfin -- Gel-staining techniques / Carl R. Merril -- Elution of protein from gels / Michael G. Harrington -- Purification of membrane proteins / Thomas C. Thomas, Mark G. McNamee -- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg, Stephen C. Francesconi, Chris Civalier, Scott S. Walker -- Purification of glycoproteins / Craig Gerard -- Purification of multienzyme complexes / Paul A. Srere, Christopher K. Mathews -- Determination of purity / David G. Rhodes, Thomas M. Laue -- Determination of size, molecular weight, and presence of subunits / Thomas M. Laue, David G. Rhodes -- Amino acid analysis / Juris Ozols -- Limited N-terminal sequence analysis / Paul Matsudaira -- Peptide mapping / Ralph C. Judd -- Analysis for protein modifications and nonprotein cofactors / Sam Seifter, Sasha Englard -- Protein crystallization / David Ollis, Stephen White -- Preparation of polyclonal antibodies / Bonnie S. Dunbar, Eric D. Schwoebel -- Preparation of monoclonal antibodies / Bonnie S. Dunbar, Sheri M. Skinner -- Protein blotting and immunodetection / Therese M. Timmons, Bonnie S. Dunbar -- Immunoprecipitation of proteins / Gary L. Firestone, Sandra D. Winguth -- Immunoassays / Charles W. Parker -- Radiolabeling of proteins / Charles W. Parker -- Using purified protein to clone its gene / John M. Wozney -- Computer analysis of protein structure / Patrick Argos -- Rethinking your purification procedure / Murray P. Deutscher -- The private life of DNA polymerase I / Arthur Kornberg -- Purification and crystallization of transaldolase from Candida utilis / B.L. Horecker, O. Tsolas -- Discovery of glutamine synthetase cascade / Earl R. Stadtman -- Discovery, resolution, purification, and function of elongation factors / Kivie Moldave.
Use copy
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for protein and enzyme purification * Personal perspectives on enzyme purification by eminent researchers Among the Topics Covered * General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
Electronic reproduction.
[Place of publication not identified] :
HathiTrust Digital Library,
2011.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
http://purl.oclc.org/DLF/benchrepro0212
English.
0121820831 9780121820831
(WaSeSS)ssj0000492525
Proteins--Purification.
Proteins.
Proteins--isolation & purification
Protéines--Purification.
Protéines.
protein.
Proteins--Purification
Proteine
Reinigung
Reinigungsverfahren
Eiwitten.
Enzymen.
Zuiveringstechnieken.
Laboratoriumonderzoek.
Proteine Reinigungsverfahren Proteine Reinigung
Electronic books.
QP601 / .M49 v.182 QP551
574.19296
577.15 N0.182
QP601.M49 / v.182
W1 [QU 55] / ME9615K v.182 1990 QU 55