Adaptive mutation in E. coli modulated by luxS
Hennessey, Michael
Adaptive mutation in E. coli modulated by luxS - Institute of Science and Technology Austria 2023
Thesis
Abstract Acknowledgements About the Author List of Publications Abbreviations and Technical Terms 1 An incomplete introduction to bacterial mutation 2 Development of the droplet platform 3 Results and Discussion 4 Methods References Appendix
Mutation rates represent the net result of complex interactions among various cellular processes and can dramatically influence the evolutionary fate of microbial populations. However, many popular techniques used to study mutations are subject to the confounding effects of heredity and the subtleties of adaptation to selection, all of which make it difficult to observe any dynamic responses of mutation rates to fitness challenges. Furthermore, in spite of the ubiquity of quorum sensing systems across the bacterial domain and relevance for many physiological behaviors, the effects of such mechanisms on mutation rate and adaptation remain poorly understood. In the following work, I present the development of a microfluidic droplet-based method to measure single base-pair mutation rates in growing populations of the bacterium Escherichia coli. I use this method to observe a stress-induced increase in mutation rate that is mediated by luxS, a highly conserved bacterial quorum sensing component. I also show that the aforementioned increase in mutation rate, and its associated control by luxS, corresponds to a higher degree of adaptability under competitive environments.
Adaptive mutation in E. coli modulated by luxS - Institute of Science and Technology Austria 2023
Thesis
Abstract Acknowledgements About the Author List of Publications Abbreviations and Technical Terms 1 An incomplete introduction to bacterial mutation 2 Development of the droplet platform 3 Results and Discussion 4 Methods References Appendix
Mutation rates represent the net result of complex interactions among various cellular processes and can dramatically influence the evolutionary fate of microbial populations. However, many popular techniques used to study mutations are subject to the confounding effects of heredity and the subtleties of adaptation to selection, all of which make it difficult to observe any dynamic responses of mutation rates to fitness challenges. Furthermore, in spite of the ubiquity of quorum sensing systems across the bacterial domain and relevance for many physiological behaviors, the effects of such mechanisms on mutation rate and adaptation remain poorly understood. In the following work, I present the development of a microfluidic droplet-based method to measure single base-pair mutation rates in growing populations of the bacterium Escherichia coli. I use this method to observe a stress-induced increase in mutation rate that is mediated by luxS, a highly conserved bacterial quorum sensing component. I also show that the aforementioned increase in mutation rate, and its associated control by luxS, corresponds to a higher degree of adaptability under competitive environments.