MARC details
| 000 -LEADER |
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| fixed length control field |
05484cam a2200769 a 4500 |
| 001 - CONTROL NUMBER |
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on1135204776 |
| 003 - CONTROL NUMBER IDENTIFIER |
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OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250702171210.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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cr cn||||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
200109s2020 nyua ob 000 0 eng |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
NLM |
| Language of cataloging |
eng |
| Transcribing agency |
NLM |
| Modifying agency |
GW5XE |
| -- |
OCLCF |
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VT2 |
| -- |
EUN |
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UKMGB |
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YDX |
| -- |
OCLCO |
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NLM |
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OCLCO |
| -- |
OCLCL |
| -- |
UKAHL |
| 015 ## - NATIONAL BIBLIOGRAPHY NUMBER |
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| National bibliography number |
GBC140916 |
| Source |
bnb |
| 016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER |
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| Record control number |
101774160 |
| Source |
DNLM |
| 016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER |
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| Record control number |
019652988 |
| Source |
Uk |
| 019 ## - |
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| -- |
1153047392 |
| -- |
1239905422 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9781493997565 |
| Qualifying information |
(electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
1493997564 |
| Qualifying information |
(electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| Canceled/invalid ISBN |
1493997556 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| Canceled/invalid ISBN |
9781493997558 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
1493997556 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9781493997558 |
| 024 3# - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
9781493997558 |
| 024 7# - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
10.1007/978-1-4939-9756-5. |
| Source of number or code |
doi |
| 029 1# - (OCLC) |
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| OCLC library identifier |
UKMGB |
| System control number |
019652988 |
| 029 1# - (OCLC) |
|---|
| OCLC library identifier |
AU@ |
| System control number |
000066472115 |
| 029 0# - (OCLC) |
|---|
| OCLC library identifier |
NLM |
| System control number |
101774160 |
| 035 ## - SYSTEM CONTROL NUMBER |
|---|
| System control number |
(OCoLC)1135204776 |
| Canceled/invalid control number |
(OCoLC)1153047392 |
| -- |
(OCoLC)1239905422 |
| 037 ## - SOURCE OF ACQUISITION |
|---|
| Stock number |
978-1-4939-9755-8 |
| Source of stock number/acquisition |
00024965 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
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| Classification number |
QL537.D76 |
| 060 00 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
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| Classification number |
QY 25 |
| 072 #7 - SUBJECT CATEGORY CODE |
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| Subject category code |
PSC. |
| Source |
bicssc |
| 072 #7 - SUBJECT CATEGORY CODE |
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| Subject category code |
SCI072000. |
| Source |
bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE |
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| Subject category code |
PSC. |
| Source |
thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
595.77/4 |
| Edition number |
23 |
| 049 ## - LOCAL HOLDINGS (OCLC) |
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| Holding library |
MAIN |
| 245 00 - TITLE STATEMENT |
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| Title |
Fundamental approaches to screen abnormalities in drosophila / |
| Statement of responsibility, etc. |
edited by Monalisa Mishra. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
New York : |
| Name of publisher, distributor, etc. |
Springer, |
| Date of publication, distribution, etc. |
c2020. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
1 online resource. |
| 336 ## - CONTENT TYPE |
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| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
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| Media type term |
computer |
| Media type code |
c |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
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| Carrier type term |
online resource |
| Carrier type code |
cr |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
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| File type |
text file |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
|---|
| Encoding format |
PDF |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Springer protocols handbooks, |
| International Standard Serial Number |
1949-2456 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc |
Includes bibliographical references. |
| 505 0# - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
Chapter 1. Preparation of normal food and modified food for Drosophila -- Chapter 2. Protocol for dissection of various tissues (gut, imaginal disc, fat body, ovary, testes) in larva and adult fly -- Chapter 3. Biochemical estimation of various metabolites (protein, lipid, carbohydrate, trehalose, cholesterol) -- Chapter 4. Detection of fat within the Drosophila body -- Chapter 5. Estimation of reactive oxygen species (ROS) production in Drosophila cells -- Chapter 6. Methods to check the metabolic activity of fly -- Chapter 7. Detection of DNA damage in larva and adult stage of Drosophila by Fluorescent microscopy -- Chapter 8. Imaging of larva/adult gut damage using Bright field microscopy -- Chapter 9. A comet assay to detect severity of DNA damage -- Chapter 10. Checking the abnormality in hemolymph by Giemsa stain -- Chapter 11. Analysis of channel proteins -- Chapter 12. Various behavioral assays in larva and adult Drosophila -- Chapter 13. Detection of various metals within the tissue by Energy dispersive X-ray (EDX) through SEM and FTIR -- Chapter 14. Imaging of various sections of fly through Scanning Electron Microscopy (SEM) -- Chapter 15. Preparation of thin sections and fixation of various tissues of Drosophila for examination by transmission electron microscopy (TEM) -- Chapter 16. Flow cytometric analysis of Drosophila cells. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided. The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila's eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Drosophila |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
95406 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Drosophila |
| General subdivision |
Genetics |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
88148 |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Drosophila |
| General subdivision |
genetics |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Drosophiles |
| Form subdivision |
Manuels de laboratoire. |
| 9 (RLIN) |
971343 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Drosophila |
| Source of heading or term |
fast |
| 9 (RLIN) |
1453 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Drosophila |
| General subdivision |
Genetics |
| Source of heading or term |
fast |
| 655 #2 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory Manual |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory manuals |
| Source of term |
fast |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory manuals. |
| Source of term |
lcgft |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Manuels de laboratoire. |
| Source of term |
rvmgf |
| 9 (RLIN) |
966128 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mishra, Monalisa, |
| Relator term |
editor. |
| 9 (RLIN) |
95409 |
| 758 ## - |
|---|
| -- |
has work: |
| -- |
Fundamental approaches to screen abnormalities in drosophila (Text) |
| -- |
https://id.oclc.org/worldcat/entity/E39PCGQjkTDJmqBdCpXbXVpr7b |
| -- |
https://id.oclc.org/worldcat/ontology/hasWork |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Relationship information |
Print version: |
| Title |
Fundamental approaches to screen abnormalities in drosophila. |
| Place, publisher, and date of publication |
New York : Springer, ©2020 |
| International Standard Book Number |
1493997556 |
| -- |
9781493997558 |
| Record control number |
(OCoLC)1102472893 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
|---|
| Uniform title |
Springer protocols (Series) |
| International Standard Serial Number |
1949-2448 |
| 9 (RLIN) |
80328 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-4939-9756-5">https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-4939-9756-5</a> |
| 938 ## - |
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Askews and Holts Library Services |
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ASKH |
| -- |
AH38372377 |
| 938 ## - |
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YBP Library Services |
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YANK |
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16603807 |
| 994 ## - |
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92 |
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ATIST |