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SFB |
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eng |
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0128142812 |
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9780128142813 |
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0128142804 |
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9780128142813 |
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532.05 |
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23 |
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| 049 ## - LOCAL HOLDINGS (OCLC) |
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MAIN |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
Microfluidics in cell biology. |
| Number of part/section of a work |
Part A, |
| Name of part/section of a work |
Microfluidics for multicellular systems / |
| Statement of responsibility, etc. |
edited by by Junsang Doh, Daniel Fletcher, Matthieu Piel. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Cambridge, Massachusetts : |
| Name of producer, publisher, distributor, manufacturer |
Academic Press, |
| Date of production, publication, distribution, manufacture, or copyright notice |
2018. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
1 online resource (276 pages). |
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text |
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txt |
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computer |
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online resource |
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cr |
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rdacarrier |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Methods in Cell Biology ; |
| Volume/sequential designation |
Volume 146 |
| 588 ## - SOURCE OF DESCRIPTION NOTE |
|---|
| Source of description note |
Description based on print version record. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Front Cover -- Microfluidics in Cell Biology Part A: Microfluidics for Multicellular Systems -- Copyright -- Contents -- Contributors -- Preface -- Section 1: Microfluidics for cell monolayers/spheroids -- Chapter 1: Tubular microscaffolds for studying collective cell migration -- 1. Introduction -- 2. Microfabrication of Microtubular Architectures for Epithelial Collective Migration Study -- 2.1. Materials -- 2.2. Equipment -- 2.3. Methods -- 2.3.1. Fabrication of PDMS microtube -- 2.3.2. Fabrication of UV-curable polymeric microtube -- 2.3.3. Preparation of elastomeric circular microchannels -- 3. Functionalization of the Tubular Microscaffolds With Extracellular Matrix Protein and Cell Seeding -- 3.1. Materials -- 3.2. Equipment -- 3.3. Methods -- 3.3.1. Functionalization of elastomeric microtubes and seeding cells procedure -- 3.3.2. Functionalization of elastomeric circular microchannels and seeding cells procedure -- 4. 3D Imaging and Image Analysis -- 4.1. Materials -- 4.2. Equipment -- 4.3. Methods -- 4.3.1. Immunostaining of epithelia in microtubes -- 4.3.2. 3D Imaging of epithelia in microtubes -- 4.3.3. Image analysis -- 5. Discussion and Perspectives -- Acknowledgments -- References -- Chapter 2: Endothelial cell monolayer-based microfluidic systems mimicking complex in vivo microenvironments for the stu -- 1. Introduction -- 2. Assembly and Characterization of a Parallel-Plate Flow Chamber -- 2.1. Materials -- 2.2. Equipment -- 2.3. Methods -- 2.3.1. Fabrication of a mechanically assembled parallel-plate flow chamber -- 2.3.2. EC culture on glass coverslips -- 2.3.3. Observation of leukocyte adhesion cascades using a parallel-plate flow chamber -- 2.3.4. Estimation of flow characteristics in a parallel-plate flow chamber -- 3. Alignment of Endothelial Monolayers Using Nanogrooved Surfaces -- 3.1. Materials -- 3.2. Equipment. |
| 505 8# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
3.3. Methods -- 4. Fabrication of Stenotic Structures and Characterization of Complex Flow Patterns in Post-stenotic Regions -- 4.1. Materials -- 4.2. Equipment -- 4.3. Methods -- 4.3.1. Fabrication of a stenotic structure -- 4.3.2. Fluidic circuit assembly and flow experiment -- 4.3.3. PIV experiment -- 4.3.4. CFD simulation -- 5. Extension to 3D Blood Vessel/Inflamed Tissue Structures -- 5.1. Materials -- 5.2. Equipment -- 5.3. Methods -- 5.3.1. EC culture on porous membranes -- 5.3.2. 3D collagen gelation -- 5.3.3. Parallel-plate flow chamber assembly and flow experiment -- 6. Summary and Outlook -- Acknowledgments -- References -- Chapter 3: Constrained spheroids/organoids in perfusion culture -- 1. Introduction -- 2. Tethered Spheroids/Organoids -- 2.1. Materials and Reagents -- 2.2. Sticky and Soft Substrate Surfaces -- 2.3. Electrospun Nanofiber-Coated Surface -- 2.4. Forming and Culturing Tethered Spheroids -- 2.5. Characterizing Tethered Spheroids -- 2.6. Potential Pitfalls and Solutions -- 3. Physically-Constrained Spheroids/Organoids -- 3.1. Materials and Reagents -- 3.2. Track-Etched Porous Membrane -- 3.3. Microfabricated Porous Ultrathin Membranes -- 3.3.1. Conjugation of PEG and galactose (AHG) to the glass coverslips -- 3.3.2. Fabrication of porous ultrathin Parylene C membrane -- 3.4. Macroporous Soft Sponges -- 3.5. Forming and Culturing Physically-Constrained Spheroids -- 3.6. Characterizing Physically-Constrained Spheroids -- 3.7. Potential Pitfalls and Solutions -- 4. Perfusion Culture in Microfluidic Channels -- 4.1. Materials and Reagents -- 4.2. Micropillars as Spheroid Trap -- 4.3. Dynamic Traps -- 4.4. Forming and Culturing Spheroids in Microfluidic Channels -- 4.5. Characterizing Spheroids in Microfluidics Channels -- 4.6. Potential Pitfalls and Solutions -- 5. Applications -- 6. Conclusions -- Acknowledgments -- References. |
| 505 8# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Section 2: Organs on chips -- Chapter 4: Generation of functional cardiac microtissues in a beating heart-on-a-chip -- 1. Introduction -- 2. Materials and Equipment -- 3. Methods -- 3.1. Master Mold Fabrication -- 3.2. Beating Heart-on-a-Chip Device Fabrication -- 3.3. iPS-CM Seeding and Mechanical Stimulation -- 3.4. Cardiac Microtissue Analysis and Readout -- References -- Chapter 5: Kidney on chips -- 1. Basic Functions of the Kidney -- 1.1. Basic Renal Function and Structure -- 1.1.1. Excretion of waste products and foreign substances -- 1.1.2. Regulation of water and electrolytes -- 1.1.3. Production of hormones -- 1.1.4. Organ crosstalk: Kidney and other organs -- 2. Kidney Tubule-on-a-Chip -- 2.1. Fluid Shear Stress in Kidney-on-a-Chip -- 2.2. Development of Kidney Tubule-on-a-Chip -- 2.3. Drug Nephrotoxicity Using Kidney Tubule-on-a-Chip -- 2.4. Disease Modeling Using Kidney Tubule-on-a-Chip -- 2.4.1. Kidney stone -- 2.4.2. Kidney fibrosis -- 2.5. Examples of Tubule-on-a-Chip for Nephrotoxicity -- 2.5.1. Device fabrication -- 2.5.2. Application of human pharmacokinetic profiles -- 3. Glomerulus-on-a-Chip -- 3.1. Development of Glomerulus-on-a-Chip -- 3.2. Drug Nephrotoxicity -- 3.3. Disease Modeling -- 3.3.1. Hypertensive nephropathy -- 3.3.2. Diabetic nephropathy -- 4. Kidney Chamber in Multi-organs-on-a-Chip -- 4.1. Kidney in Multi-organs-on-a-Chips -- 4.2. Drug Pharmacokinetics -- 4.3. Organ Interaction and Disease Modeling -- 5. Future Perspective -- Acknowledgment -- References -- Chapter 6: Liver sinusoid on a chip -- 1. Introduction of Cell-Cell Interactions in Liver Sinusoidal Microenvironments -- 1.1. Architecture and Cell Composition of the Liver Sinusoids -- 1.2. Cellular Interactions in the Liver Sinusoids During Fibrosis and Inflammation -- 1.3. Sinusoidal Mechanical Microenvironments. |
| 505 8# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
1.4. In Vitro Models of the Liver Sinusoids -- 2. Construction of the Liver Sinusoid Chip -- 2.1. Microfluidic Device Fabrication -- 2.2. Primary Hepatic Cell Isolation -- 2.3. Identification of Isolated Hepatic Cells -- 2.4. Chip Assembling and Characterization -- 3. Mechanical Microenvironment Analysis -- 3.1. Fluidic Dynamic Model -- 3.2. Fluid Field Visualization -- 4. Liver-Specific Functions of the Sinusoidal Chip -- 5. Hepatic Immune Response -- 5.1. Neutrophil Isolation -- 5.2. Neutrophil Recruitment in Chip -- 6. Conclusive Remarks and Future Perspectives -- Acknowledgments -- References -- Further Reading -- Chapter 7: Pathomimetic modeling of human intestinal diseases and underlying host-gut microbiome interactions in a gut-on-a-.. -- 1. Introduction -- 2. Microfabrication of a Gut-on-a-Chip Device -- 2.1. Materials -- 2.2. Equipment -- 2.3. Methods -- 3. Recreation of Intestinal Microenvironment and Physiology -- 3.1. Materials -- 3.2. Equipment -- 3.3. Methods -- 4. Recapitulation of Host-Gut Microbiome Intercellular Interactions -- 4.1. Materials -- 4.2. Methods -- 5. Pathomimetic Modeling I: Intestinal Inflammation -- 5.1. Materials -- 5.2. Equipment -- 5.3. Methods -- 6. Pathomimetic Modeling II: Intestinal Bacterial Overgrowth -- 6.1. Methods -- 7. Outlook -- Acknowledgments -- References -- Chapter 8: 3D in vitro microvascular model-based lymphoma model -- 1. Introduction -- 2. Materials and Equipment -- 2.1. Materials -- 2.2. Equipment -- 3. Methods -- 3.1. Fabrication of DLBCL-on-Chip Model -- 3.2. Endothelialization of DLBCL-on-Chip Model -- 3.3. Plasma Bonding -- 3.4. Tumor Cell Generation -- 4. Closing Remarks -- References -- Chapter 9: Blood-brain barrier on a chip -- 1. Introduction -- 2. The Endogenous BBB -- 2.1. Cellular Makeup of the BBB -- 2.2. Endothelial Cells -- 2.3. Pericytes and Smooth Muscle Cells. |
| 505 8# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
2.4. Astrocytes -- 2.5. Neurons -- 2.6. Microglia -- 3. Current BBB Models -- 4. BBB-on-Chip Models -- 4.1. Protocol -- 5. Considerations for Modeling the BBB on a Chip -- 5.1. Types of Cells Used -- 5.2. Transendothelial Electrical Resistance Values -- 5.3. Barrier Permeability -- 5.4. Cellular Functionality -- 5.5. Material Biocompatibility -- 5.6. Cellular Adhesion and Cell Growth -- 5.7. Fluid Flow -- 6. Conclusion -- References -- Chapter 10: Pharmacokinetic-based multi-organ chip for recapitulating organ interactions -- 1. Introduction -- 2. Materials and Equipment -- 2.1. Chip Fabrication -- 2.2. Cell Seeding and Culture on Chip -- 2.3. Evaluation of Cell Viability -- 2.4. Evaluation of Metabolites -- 2.5. Construction of PK Model -- 3. Methods -- 3.1. Chip Fabrication -- 3.2. Cell Seeding and Culture on Chip -- 3.3. Evaluation of Cell Viability -- 3.4. Evaluation of Metabolites -- 3.5. Construction of PK Model -- 4. Exemplary MATLAB Code for Liver-Tumor-Blood Chip -- 5. Final Remarks -- Acknowledgment -- References -- Chapter 11: Studying TCR T cell anti-tumor activity in a microfluidic intrahepatic tumor model -- 1. Introduction to Microfluidic Technology -- 1.1. Main Advantages of Microfluidics -- 1.2. Main Limitations of Microfluidics -- 1.3. Microfluidic Models for Cancer Immunology and Immunotherapy -- 2. Materials and Equipment -- 2.1. Device Fabrication and Surface Coating -- 2.2. Cell Culture and Cell Handling -- 2.3. Microfluidic Assay -- 2.4. Cell Labeling, Confocal Imaging and Data Analysis -- 3. Methods -- 3.1. Device Fabrication -- 3.2. Surface Coating of the Microchannels With PDL -- 3.3. Transduced TCR T Cells -- 3.4. Electroporated TCR T Cells -- 3.5. Monocyte Isolation -- 3.6. Cancer Cell Culture and Cancer Aggregate Formation -- 3.7. Generation of a Tumor Microenvironment in a Microfluidic Device. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Microfluidics. |
| 9 (RLIN) |
84193 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cell physiology. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cytology |
| General subdivision |
Technique. |
| 9 (RLIN) |
2008 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Labs on a chip. |
| 9 (RLIN) |
966562 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Microfluidic devices. |
| 9 (RLIN) |
90553 |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Microfluidics |
| 9 (RLIN) |
84193 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cell Physiological Phenomena |
| 9 (RLIN) |
13220 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cytological Techniques |
| 9 (RLIN) |
2010 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Lab-On-A-Chip Devices |
| 9 (RLIN) |
84826 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Microfluidic Analytical Techniques |
| 9 (RLIN) |
84127 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Microfluidique. |
| 9 (RLIN) |
483450 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cellules |
| General subdivision |
Physiologie. |
| 9 (RLIN) |
97928 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cytologie |
| General subdivision |
Technique. |
| 9 (RLIN) |
100145 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Laboratoires sur puces. |
| 9 (RLIN) |
966563 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Dispositifs microfluidiques. |
| 9 (RLIN) |
539957 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
TECHNOLOGY & ENGINEERING / Hydraulics. |
| Source of heading or term |
bisacsh |
| 9 (RLIN) |
196456 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Microfluidic devices |
| Source of heading or term |
fast |
| 9 (RLIN) |
90553 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Labs on a chip |
| Source of heading or term |
fast |
| 9 (RLIN) |
966562 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cytology |
| General subdivision |
Technique |
| Source of heading or term |
fast |
| 9 (RLIN) |
2008 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Cell physiology |
| Source of heading or term |
fast |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Microfluidics |
| Source of heading or term |
fast |
| 9 (RLIN) |
84193 |
| 655 #4 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Internet Resources. |
| 9 (RLIN) |
80513 |
| 655 #4 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Index not Present. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Doh, Junsang, |
| Relator term |
editor. |
| 9 (RLIN) |
916736 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Fletcher, Daniel, |
| Relator term |
editor. |
| 9 (RLIN) |
916737 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Piel, Matthieu, |
| Relator term |
editor. |
| -- |
https://id.oclc.org/worldcat/entity/E39PCjv9dtRdKDXHJj86cdXtVd |
| 9 (RLIN) |
179952 |
| 758 ## - |
|---|
| -- |
has work: |
| -- |
Microfluidics in cell biology Microfluidics for multicellular systems Part A (Text) |
| -- |
https://id.oclc.org/worldcat/entity/E39PCG9qj6RDHhPGtdpXyjdkH3 |
| -- |
https://id.oclc.org/worldcat/ontology/hasWork |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Qualifying information |
Original |
| International Standard Book Number |
0128142804 |
| -- |
9780128142806 |
| Record control number |
(OCoLC)1029805270 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
|---|
| Uniform title |
Methods in cell biology ; |
| Volume number/sequential designation |
Volume 146. |
| 9 (RLIN) |
100821 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/0091679X/146">https://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/0091679X/146</a> |
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cloudLibrary |
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CLDL |
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9780128142813 |
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YBP Library Services |
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YANK |
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15861290 |
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EBSCOhost |
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EBSC |
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1797038 |
| 938 ## - |
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YBP Library Services |
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YANK |
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15608211 |
| 936 ## - OCLC/CONSER MISCELLANEOUS DATA (OCLC); PIECE USED FOR CATALOGING (pre-AACR2) (RLIN) |
|---|
| OCLC control number(s) of parallel record(s) (OCLC); Piece used for cataloging, PUC (RLIN) |
BATCHLOAD |
| 994 ## - |
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92 |
| -- |
ATIST |