A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy (Record no. 655801)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02336ntm a22003377a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | AT-ISTA |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20230712103012.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 230712s2023 au ||||| m||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | ISTA |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Michalska, Julia Magdalena |
| 9 (RLIN) | 972417 |
| 245 ## - TITLE STATEMENT | |
| Title | A versatile toolbox for the comprehensive analysis of nervous tissue organization with light microscopy |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher, distributor, etc. | Institute of Science and Technology |
| Date of publication, distribution, etc. | 2023 |
| 500 ## - GENERAL NOTE | |
| General note | Thesis |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Abstract |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | About the author |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Acknowledgments |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | List of publications |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Table of Contents |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | List of abbreviations |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | List of figures |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | List of tables |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 1 Introduction |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 2 Method development: Comprehensive analysis of nervous tissue across scales |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 3 Studying the hippocampal circuitry with CATS |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 4 Outlook |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 5 Bibliography |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 6 Appendix |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The brain is an exceptionally sophisticated organ consisting of billions of cells and trillions of connections that orchestrate our cognition and behavior. To decode its complex connectivity, it is pivotal to disentangle its intricate architecture spanning from cm-sized circuits down to tens of nm-small synapses. To achieve this goal, I developed CATS – Comprehensive Analysis of nervous Tissue across Scales, a versatile toolbox for obtaining a holistic view of nervous tissue context with (superresolution) fluorescence microscopy. CATS combines comprehensive labeling of the extracellular space, that is compatible with chemical fixation, with information on molecular markers, superresolved data acquisition and machine-learning based data analysis for segmentation and synapse identification. I used CATS to analyze key features of nervous tissue connectivity, ranging from whole tissue architecture, neuronal in- and output-fields, down to synapse morphology. Focusing on the hippocampal circuitry, I quantified synaptic transmission properties of mossy fiber boutons and analyzed the connectivity pattern of dentate gyrus granule cells with CA3 pyramidal neurons. This shows that CATS is a viable tool to study hallmarks of neuronal connectivity with light microscopy. |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.15479/at:ista:12470">https://doi.org/10.15479/at:ista:12470</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not Lost | Dewey Decimal Classification | Library | Library | 12/07/2023 | Quiet Room | AT-ISTA#002835 | 16/09/2025 | 12/07/2023 | Book |