MARC details
| 000 -LEADER |
| fixed length control field |
05547cam a2200625 i 4500 |
| 001 - CONTROL NUMBER |
| control field |
on1348179325 |
| 003 - CONTROL NUMBER IDENTIFIER |
| control field |
OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
| control field |
20250702171632.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
| fixed length control field |
m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
| fixed length control field |
cr cn||||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
| fixed length control field |
221019s2023 nyua ob 001 0 eng |
| 040 ## - CATALOGING SOURCE |
| Original cataloging agency |
NLM |
| Language of cataloging |
eng |
| Description conventions |
rda |
| -- |
pn |
| Transcribing agency |
NLM |
| Modifying agency |
OCLCF |
| -- |
WSU |
| -- |
GW5XE |
| -- |
OCLCO |
| 016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER |
| Record control number |
9918751185206676 |
| Source |
DNLM |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
| International Standard Book Number |
9781071626634 |
| Qualifying information |
(electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
| International Standard Book Number |
1071626639 |
| Qualifying information |
(electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
| Canceled/invalid ISBN |
9781071626627 |
| 024 8# - OTHER STANDARD IDENTIFIER |
| Standard number or code |
10.1007/978-1-0716-2663-4 |
| Source of number or code |
doi |
| 029 0# - (OCLC) |
| OCLC library identifier |
NLM |
| System control number |
9918751185206676 |
| 035 ## - SYSTEM CONTROL NUMBER |
| System control number |
(OCoLC)1348179325 |
| 042 ## - AUTHENTICATION CODE |
| Authentication code |
pcc |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
| Classification number |
QH581.2 |
| 060 00 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
| Classification number |
W1 |
| Item number |
ME9616J v.2563 2023 |
| 060 10 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
| Classification number |
QU 25 |
| 072 #7 - SUBJECT CATEGORY CODE |
| Subject category code |
PSB |
| Source |
bicssc |
| 072 #7 - SUBJECT CATEGORY CODE |
| Subject category code |
SCI007000 |
| Source |
bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE |
| Subject category code |
PSB |
| Source |
thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
| Classification number |
571.6/5 |
| Edition number |
23/eng/20221019 |
| 049 ## - LOCAL HOLDINGS (OCLC) |
| Holding library |
MAIN |
| 245 00 - TITLE STATEMENT |
| Title |
Phase-separated biomolecular condensates : |
| Remainder of title |
methods and protocols / |
| Statement of responsibility, etc. |
edited by Huan-Xiang Zhou, Jan-Hendrik Spille, Priya R. Banerjee. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
| Place of production, publication, distribution, manufacture |
New York, NY : |
| Name of producer, publisher, distributor, manufacturer |
Humana Press, |
| Date of production, publication, distribution, manufacture, or copyright notice |
[2023] |
| 300 ## - PHYSICAL DESCRIPTION |
| Extent |
1 online resource (1 PDF file (xiv, 449 pages)) : |
| Other physical details |
illustrations. |
| 336 ## - CONTENT TYPE |
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
| Media type term |
computer |
| Media type code |
c |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
| Carrier type term |
online resource |
| Carrier type code |
cr |
| Source |
rdacarrier |
| 490 1# - SERIES STATEMENT |
| Series statement |
Methods in molecular biology, |
| International Standard Serial Number |
1940-6029 ; |
| Volume/sequential designation |
2563 |
| 490 1# - SERIES STATEMENT |
| Series statement |
Springer protocols |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
| Bibliography, etc |
Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE |
| Formatted contents note |
Calculating Binodals and Interfacial Tension of Phase-Separated Condensates from Molecular Simulations, with Finite-Size Corrections -- Field-Theoretic Simulation Method to Study the Liquid-Liquid Phase Separation of Polymers -- Numerical Techniques for Applications of Analytical Theories to Sequence-Dependent Phase Separations of Intrinsically Disordered Proteins -- An Introduction to the Stickers-and-Spacers Framework as Applied to Biomolecular Condensates -- Multi-Scale Modeling of Protein-RNA Condensation In and Out of Equilibrium -- Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates -- Single-Molecule Fluorescence Methods to Study Protein-RNA Interactions Underlying Biomolecular Condensates -- Fluorescence Correlation Spectroscopy and Phase Separation -- Measurement of Protein and Nucleic Acid Diffusion Coefficients within Biomolecular Condensates Using In-Droplet Fluorescence Correlation Spectroscopy -- Single-Molecule Imaging of the Phase Separation-Modulated DNA Compaction to Study Transcriptional Repression -- Phase Separation-Based Biochemical Assays for Biomolecular Interactions -- Determining Thermodynamic and Material Properties of Biomolecular Condensates by Confocal Microscopy and Optical Tweezers -- A High-Throughput Method to Profile Protein Liquid-Liquid Phase Separation -- Phase Separation of Rubisco by the Folded SSUL Domains of CcmM in Beta-Carboxysome Biogenesis -- Cryo-Electron Tomography of Reconstituted Biomolecular Condensate -- Sedimentation Assays to Assess the Impact of Post-Translational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells -- Synthetic Organelles for Multiple mRNA Selective Genetic Code Expansion in Eukaryotes -- Single Molecule Tracking of RNA Polymerase In and Out of Condensates in Live Bacterial Cells -- An Optogenetic Toolkit for the Control of Phase Separation in Living Cells -- Assessing the Phase Separation Propensity of Proteins in Living Cells Through Optodroplet Formation -- Mass Balance Imaging: A Phase Portrait Analysis for Characterizing Growth Kinetics of Biomolecular Condensates -- Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo. |
| 520 ## - SUMMARY, ETC. |
| Summary, etc. |
This volume provides readers with a broad collection of theoretical, computational, and experimental methods to quantitatively study the properties of phase-separate biomolecular condensates in diverse systems. The chapters in this book cover topics such as theoretical and computational methods; methods for in vitro characterization of biomolecular condensates; and techniques that enable in-cell characterization of biomolecular condensates. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Phase-Separated Biomolecular Condensates: Methods and Protocols is a valuable resource that helps researchers learn and use established methods to study both biophysical properties and biological functions of biomolecular condensates. . |
| 588 0# - SOURCE OF DESCRIPTION NOTE |
| Source of description note |
Description based on online resource; title from PDF title page (SpringerLink, viewed October 19, 2022). |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name as entry element |
Cell organelles |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
975339 |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name as entry element |
Biomolecular Condensates |
| 9 (RLIN) |
975340 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
| Topical term or geographic name as entry element |
Cell organelles |
| Source of heading or term |
fast |
| 9 (RLIN) |
85090 |
| 655 #2 - INDEX TERM--GENRE/FORM |
| Genre/form data or focus term |
Laboratory Manual |
| 655 #7 - INDEX TERM--GENRE/FORM |
| Genre/form data or focus term |
Laboratory manuals |
| Source of term |
fast |
| 655 #7 - INDEX TERM--GENRE/FORM |
| Genre/form data or focus term |
Laboratory manuals. |
| Source of term |
lcgft |
| 655 #7 - INDEX TERM--GENRE/FORM |
| Genre/form data or focus term |
Manuels de laboratoire. |
| Source of term |
rvmgf |
| 9 (RLIN) |
966128 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Zhou, Huan-Xiang, |
| Relator term |
editor. |
| 9 (RLIN) |
975341 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Spille, Jan-Hendrik, |
| Relator term |
editor. |
| 9 (RLIN) |
975342 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
| Personal name |
Banerjee, Priya R. |
| Relator term |
editor. |
| 9 (RLIN) |
975343 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
| Uniform title |
Methods in molecular biology (Clifton, N.J.) ; |
| Volume number/sequential designation |
v. 2563. |
| International Standard Serial Number |
1064-3745 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
| Uniform title |
Springer protocols (Series) |
| International Standard Serial Number |
1949-2448 |
| 9 (RLIN) |
80328 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
| Uniform Resource Identifier |
<a href="https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-0716-2663-4">https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-0716-2663-4</a> |
| 994 ## - |
| -- |
92 |
| -- |
ATIST |