MARC details
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07660cam a2200973Ma 4500 |
| 001 - CONTROL NUMBER |
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ocn763161431 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20241002154442.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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m d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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cr cn||||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
111109s2012 ne a obf 001 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
E7B |
| Language of cataloging |
eng |
| Description conventions |
pn |
| Transcribing agency |
E7B |
| Modifying agency |
OCLCQ |
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CDX |
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NTE |
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CUX |
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REDDC |
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OCLCQ |
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N$T |
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OCLCQ |
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DEBSZ |
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OCLCQ |
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YDXCP |
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OPELS |
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OCLCQ |
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OCLCA |
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OPELS |
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U3G |
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OCLCO |
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WAU |
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OCLCQ |
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OCLCO |
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OCL |
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OCLCO |
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OCLCQ |
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OCLCO |
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OPELS |
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OCLCQ |
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ICA |
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MERUC |
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OPELS |
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K6U |
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ESU |
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OCLCQ |
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LEATE |
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BUF |
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OCLCO |
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WRM |
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OCLCO |
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OCLCQ |
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CHVBK |
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FQM |
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OCLCO |
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YDX |
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OCLCO |
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INT |
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OCLCA |
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AU@ |
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OCLCQ |
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OCLCO |
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OTZ |
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WYU |
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OCLCO |
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OCLCQ |
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OCLCO |
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M8D |
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OCLCO |
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OCLCA |
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UKCRE |
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S2H |
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OCLCO |
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OCLCQ |
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UKAHL |
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OCLCO |
| 019 ## - |
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| -- |
776632410 |
| -- |
828058575 |
| -- |
877017299 |
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972137035 |
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972285082 |
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992021760 |
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1037765019 |
| -- |
1038700669 |
| -- |
1083602336 |
| -- |
1153501356 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9780123855459 |
| Qualifying information |
(electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
0123855454 |
| Qualifying information |
(electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| Canceled/invalid ISBN |
9780123855442 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| Canceled/invalid ISBN |
0123855446 |
| 035 ## - SYSTEM CONTROL NUMBER |
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| System control number |
453823 |
| -- |
(N$T) |
| 035 ## - SYSTEM CONTROL NUMBER |
|---|
| System control number |
(OCoLC)763161431 |
| Canceled/invalid control number |
(OCoLC)776632410 |
| -- |
(OCoLC)828058575 |
| -- |
(OCoLC)877017299 |
| -- |
(OCoLC)972137035 |
| -- |
(OCoLC)972285082 |
| -- |
(OCoLC)992021760 |
| -- |
(OCoLC)1037765019 |
| -- |
(OCoLC)1038700669 |
| -- |
(OCoLC)1083602336 |
| -- |
(OCoLC)1153501356 |
| 037 ## - SOURCE OF ACQUISITION |
|---|
| Stock number |
1104879:10110003 |
| Source of stock number/acquisition |
Elsevier Science & Technology |
| Note |
http://www.sciencedirect.com |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
QH506 |
| Item number |
.C36 2012eb |
| 060 10 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
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| Classification number |
QU 25 |
| 072 #7 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
SCI |
| Subject category code subdivision |
049000 |
| Source |
bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
572.8/078 |
| Edition number |
23 |
| 084 ## - OTHER CLASSIFICATION NUMBER |
|---|
| Classification number |
WC 4150 |
| Number source |
rvk |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
MAIN |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Carson, Sue |
| Fuller form of name |
(Susan) |
| 9 (RLIN) |
169047 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Molecular biology techniques : |
| Remainder of title |
a classroom laboratory manual / |
| Statement of responsibility, etc. |
Susan Carson, Heather Miller, D. Scott Witherow. |
| 250 ## - EDITION STATEMENT |
|---|
| Edition statement |
3rd ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Amsterdam ; |
| -- |
Boston : |
| Name of publisher, distributor, etc. |
Elsevier /Academic Press, |
| Date of publication, distribution, etc. |
©2012. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
1 online resource (xxvi, 200 pages) : |
| Other physical details |
color illustrations. |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
computer |
| Media type code |
c |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
online resource |
| Carrier type code |
cr |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
|---|
| File type |
data file |
| Source |
rda |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
ClinicalKey |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc |
Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. |
| 588 0# - SOURCE OF DESCRIPTION NOTE |
|---|
| Source of description note |
Print version record. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions. |
| 590 ## - LOCAL NOTE (RLIN) |
|---|
| Local note |
WorldCat record variable field(s) change: 650 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology |
| Form subdivision |
Laboratory manuals. |
| 9 (RLIN) |
80278 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology |
| General subdivision |
Technique |
| Form subdivision |
Handbooks, manuals, etc. |
| 9 (RLIN) |
105378 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Recombinant DNA. |
| 9 (RLIN) |
87207 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Protein engineering. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
DNA, Recombinant |
| 9 (RLIN) |
80872 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular Biology |
| General subdivision |
methods |
| 9 (RLIN) |
23201 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Protein Engineering |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biologie moléculaire |
| Form subdivision |
Manuels de laboratoire. |
| 9 (RLIN) |
115585 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biologie moléculaire |
| General subdivision |
Technique |
| Form subdivision |
Guides, manuels, etc. |
| 9 (RLIN) |
1039889 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
ADN recombinant. |
| 9 (RLIN) |
100045 |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Technologie des protéines. |
| 9 (RLIN) |
83862 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
SCIENCE |
| General subdivision |
Life Sciences |
| -- |
Molecular Biology. |
| Source of heading or term |
bisacsh |
| 9 (RLIN) |
19012 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology. |
| Source of heading or term |
fast |
| Authority record control number |
(OCoLC)fst01024734 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular biology |
| General subdivision |
Technique. |
| Source of heading or term |
fast |
| Authority record control number |
(OCoLC)fst01024758 |
| 9 (RLIN) |
83033 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Methode |
| Source of heading or term |
gnd |
| 9 (RLIN) |
2960 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molekularbiologie |
| Source of heading or term |
gnd |
| 9 (RLIN) |
1147 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Methode |
| General subdivision |
(DE-588c)4038971-6. |
| Source of heading or term |
swd |
| 9 (RLIN) |
1039890 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molekularbiologie |
| General subdivision |
(DE-588c)4039983-7. |
| Source of heading or term |
swd |
| 9 (RLIN) |
1039891 |
| 655 #0 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Electronic books. |
| 9 (RLIN) |
396 |
| 655 #2 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Handbook |
| 9 (RLIN) |
84329 |
| 655 #2 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory Manual |
| 655 #4 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Electronic books. |
| 9 (RLIN) |
396 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
handbooks. |
| Source of term |
aat |
| 9 (RLIN) |
126663 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
manuals (instructional materials) |
| Source of term |
aat |
| 9 (RLIN) |
971902 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Handbooks and manuals. |
| Source of term |
fast |
| Authority record control number |
(OCoLC)fst01423877 |
| 9 (RLIN) |
6671 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory manuals. |
| Source of term |
fast |
| Authority record control number |
(OCoLC)fst01920776 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Handbooks and manuals. |
| Source of term |
lcgft |
| 9 (RLIN) |
6671 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Laboratory manuals. |
| Source of term |
lcgft |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Guides et manuels. |
| Source of term |
rvmgf |
| 9 (RLIN) |
849768 |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Manuels de laboratoire. |
| Source of term |
rvmgf |
| 9 (RLIN) |
966128 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Miller, Heather B. |
| 9 (RLIN) |
169053 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Witherow, D. Scott. |
| 9 (RLIN) |
169054 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Relationship information |
Print version: |
| Main entry heading |
Carson, Sue (Susan). |
| Title |
Molecular biology techniques. |
| Edition |
3rd ed. |
| Place, publisher, and date of publication |
Oxford ; Waltham, MA : Academic Press, 2012 |
| International Standard Book Number |
9780123855442 |
| Record control number |
(DLC) 2012359021 |
| -- |
(OCoLC)754167841 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
|---|
| Uniform title |
ClinicalKey. |
| 9 (RLIN) |
124548 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Materials specified |
EBSCOhost |
| Uniform Resource Identifier |
<a href="https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=453823">https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=453823</a> |
| 938 ## - |
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Askews and Holts Library Services |
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ASKH |
| -- |
AH23092886 |
| 938 ## - |
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Coutts Information Services |
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COUT |
| -- |
19836982 |
| -- |
60.99 GBP |
| 938 ## - |
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ebrary |
| -- |
EBRY |
| -- |
ebr10509200 |
| 938 ## - |
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EBSCOhost |
| -- |
EBSC |
| -- |
453823 |
| 938 ## - |
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YBP Library Services |
| -- |
YANK |
| -- |
7222699 |
| 994 ## - |
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| -- |
92 |
| -- |
N$T |