MARC details
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04599ntm a22002897a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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AT-ISTA |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250911150717.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250911s2024 au ||||| m||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
ISTA |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Kelemen, Reka |
| 9 (RLIN) |
1084215 |
| 245 ## - TITLE STATEMENT |
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| Title |
Characterizing the sequence and expression evolution of the t-haplotype, a model meiotic driver |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Name of publisher, distributor, etc. |
Institute of Science and Technology Austria |
| Date of publication, distribution, etc. |
2024 |
| 500 ## - GENERAL NOTE |
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| General note |
Thesis |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
Abstract |
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| Formatted contents note |
Acknowledgements |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
About the Author |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
List of Collaborators and Publications |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
Table of Contents |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
1 Introduction |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
2 Complex history and differentiation patterns of the t-haplotype, a mouse meiotic driver |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
3 Novel patterns of expression and recruitment of new genes on the t-haplotype, a mouse selfish chromosome |
| 505 ## - FORMATTED CONTENTS NOTE |
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4 Single-nucleus RNA sequencing uncovers candidate poisons and antidotes in testes carrying the t-haplotype, a model meiotic driver |
| 505 ## - FORMATTED CONTENTS NOTE |
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| Formatted contents note |
5 Discussion |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Genomes are shaped by natural selection at the level of the organism, as genomic variants that have a beneficial effect on the viability or fecundity of their carriers are on average expected to be passed on to more offspring than less beneficial alleles. However, selection also favors genomic variants that drive their own transmission to the next generation above the mendelian expectation of 50 percent in heterozygotes, even if these self-promoting variants are less beneficial to the organism than other variants at the same locus. Such variants, called meiotic drivers, are found in diverse taxa, and often impose fitness costs on their host organisms. As meiotic drivers often require multiple genes and sequences for transmission ratio distortion, they are often found in regions of low recombination, such as inversions, which prevent their recombination with the non-driving homologous regions. Reduced recombination rates are expected to lead to the accumulation of deleterious mutations, which may affect hundreds of genes trapped in the inversions of meiotic drivers. Although the observed fitness costs of self-promoting haplotypes are thought to possibly reflect sequence degeneration, no study has systematically investigated the level of degeneration on a meiotic driver. Further, the low rates of recombination between driving and non-driving haplotypes have limited the power of traditional genetic studies in uncovering the gene content of meiotic drivers, and made the the identification of the genes causing transmission ratio distortion difficult. After an introduction to meiotic drivers in Chapter 1, this thesis presents three studies that make use of next generation sequencing data to characterize the sequence and expression evolution of genes on the t-haplotype, a large and ancient meiotic driver in house mice that is transmitted to up to 100% of the offspring in males heterozygous for it. Chapter 2 presents a comprehensive assessment of the t-haplotype’s sequence evolution, which shows signs of sequence degeneration counteracted by occasional recombination with the non-driving homolog over large parts of the meiotic driver, proposing an explanation for its long-term survival. Chapter 3 investigates the sequence and expression evolution of genes on the t-haplotype, and finds widespread expression and copy number changes and signs of less efficient purifying selection compared to the genes on the non-driving homolog. Further, this chapter finds candidates for involvment in drive: two positively selected genes on the t-haplotype, and the discovery of a t-specific gene duplicate, which was gained from another chromosome, and which acquired novel sequence and testis-specific expression on the t-haplotype. Finally, Chapter 4 provides unprecedented insights into the gene expression landscape in testes of t-carrier mice, using single nucleus sequencing. Cell-resolved RNA-sequencing allows the comparison of expression in spermatids carrying or not carrying the t-haplotype as well as the timing of t-haplotype-induced expression changes along spermatogenesis. This study shows the timing of previously found drive-associated genes, and uncovers novel candidate genes and biological processes that may underlie the complex biology of transmission ratio distortion of the t-haplotype. Chapter 5 synthesizes the findings of the three studies, and discusses them in the context of the current state of meiotic drive research. |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://doi.org/10.15479/at:ista:17119">https://doi.org/10.15479/at:ista:17119</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Dewey Decimal Classification |