Plant hormone protocols / edited by Gregory A. Tucker and Jeremy A. Roberts.

Includes bibliographical references.

Extraction and purification of an enzyme potentially involved in ABA biosynthesis -- Differential display: analysis of gene expression during plant development -- Abscisic acid: ABA immunoassay and gas chromatography/mass spectrometry verificatin -- Auxin analysis -- Photoacoustic and photothermal detection of the plant hormone ethylene -- Analysis of gibberellins -- Cytokinins: extraction, separation, and analysis -- Binding studies -- Mutagenesis -- The identification of ethene biosynthetic genes by gene silencing: antisense transgenes, agrobacterium-mediated transformation, and the tomato ACC oxidase cDNA -- Extraction, separation, and analysis of plant phosphoinositides and complex glycolipids -- Reverse genetics: screening plant populations for gene knockouts.

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In Plant Hormone Protocols, established investigators from around the world describe in step-by-step detail their best techniques for the study of plant hormones and their regulatory activities. These state-of-the-art methods include contemporary approaches to identifying the biosynthetic pathways of plant hormones, monitoring their levels, characterizing the receptors with which they interact, and analyzing of the signaling systems by which they exert their effects. Each protocol-written by a hands-on master of the technique so as to achieve robust and readily reproducible results-provides detailed instructions, a full materials list, and notes about pitfalls to avoid and the troubleshooting of unexpected problems. Comprehensive and fully detailed for reproducible laboratory success, Plant Hormone Protocols offers plant biologists an indispensable compendium of today's most powerful methods and strategies for studying plant hormones, their regulation, and their activities.