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Transgenesis techniques : principles and protocols / edited by Alan R. Clarke.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 180.Publication details: Totowa, NJ : Humana Press, ©2002.Edition: 2nd edDescription: 1 online resource (x, 351 pages) : illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781592591787
  • 0896036960
  • 9780896036963
  • 0585405379
  • 9780585405377
  • 1592591787
  • 9786610821402
  • 6610821402
  • 128082140X
  • 9781280821400
  • 0896032450
  • 9780896032453
  • 1489939016
  • 9781489939012
  • 9781489939005
  • 1489939008
Subject(s): Genre/Form: Additional physical formats: Print version:: Transgenesis techniques.DDC classification:
  • 576.5/07/24 21
LOC classification:
  • QH442.6 .T66 2002
NLM classification:
  • W1
  • QH 442.6
Online resources:
Contents:
pt. 1. Topical reviews in transgenesis -- Biomedical and agricultural applications of animal transgenesis / Jim McWhir -- pt. 2. Transgenesis in invertebrate and lower vertebrate species -- Gene transfer in Drosophila / Mark J. O'Connor -- pt. 3. Transgenesis in the mouse: oocyte injection -- Oocyte injection in the mouse / Gary A.J. Brown -- pt. 4. Alternative routes to the germline -- Adenoviral infection / Tohru Tsukui -- Retroviral infection / Richard A. Bowen -- pt. 4. Transgenesis in the mouse: the ES cell route -- In vitro isolation of murine embryonic stem cells / David Wells -- Production of chimeras derived from murine embryonic stem cells / David Wells -- Gene targeting strategies / David W. Melton -- Cre/loxP recombination system and gene targeting / Ralf Kühn -- pt. 6. Cryopreservation of mouse lines -- Cryopreservation of transgenic mouse lines / Jillian M. Shaw -- Ovarian tissue transplantation and cryopreservation: application to maintenance and recovery of transgenic and inbred mouse lines / Jillian M. Shaw -- pt. 7. Transgenesis in the rat -- Transgenesis in the rat / Linda J. Mullins -- pt. 8. Transgenesis in domestic species -- Generation of transgenic livestock by pronuclear injection / A. John Clark -- Transgenic sheep from cultured cells / Keith H.S. Campbell -- pt. 9. Characterization and analysis of transgenic strains -- Analysis of transgenic mice / Stefan Selbert.
Summary: The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.
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Includes bibliographical references and index.

pt. 1. Topical reviews in transgenesis -- Biomedical and agricultural applications of animal transgenesis / Jim McWhir -- pt. 2. Transgenesis in invertebrate and lower vertebrate species -- Gene transfer in Drosophila / Mark J. O'Connor -- pt. 3. Transgenesis in the mouse: oocyte injection -- Oocyte injection in the mouse / Gary A.J. Brown -- pt. 4. Alternative routes to the germline -- Adenoviral infection / Tohru Tsukui -- Retroviral infection / Richard A. Bowen -- pt. 4. Transgenesis in the mouse: the ES cell route -- In vitro isolation of murine embryonic stem cells / David Wells -- Production of chimeras derived from murine embryonic stem cells / David Wells -- Gene targeting strategies / David W. Melton -- Cre/loxP recombination system and gene targeting / Ralf Kühn -- pt. 6. Cryopreservation of mouse lines -- Cryopreservation of transgenic mouse lines / Jillian M. Shaw -- Ovarian tissue transplantation and cryopreservation: application to maintenance and recovery of transgenic and inbred mouse lines / Jillian M. Shaw -- pt. 7. Transgenesis in the rat -- Transgenesis in the rat / Linda J. Mullins -- pt. 8. Transgenesis in domestic species -- Generation of transgenic livestock by pronuclear injection / A. John Clark -- Transgenic sheep from cultured cells / Keith H.S. Campbell -- pt. 9. Characterization and analysis of transgenic strains -- Analysis of transgenic mice / Stefan Selbert.

The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.

English.

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