In vitro mutagenesis protocols / edited by Jeff Braman.

Includes bibliographical references and index.

Rapid and Reliable Site-Directed Mutagenesis Using Kunkel's Approach / Priya Handa, Swapna Thanedar, and Umesh Varshney -- Site-Directed Mutagenesis Using Altered b-Lactamase Specificity / Christine A. Andrews and Scott A. Lesley -- Site-Directed Mutagenesis Facilitated by DpnI Selection on Hemimethylated DNA / Fusheng Li and James I. Mullins -- Multiple Site-Directed Mutagenesis In Vitro / Yang-Gyun Kim and Stefan Maas -- Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikChange Site-Directed Mutagenesis / Wenyan Wang and Bruce A. Malcolm -- Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids / Susan A. Nadin-Davis -- Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis : Versatile Strategy to Move Beyond Structural Limitations of 20 Natural Amino Acids Side Chains in Protein Engineering / Grace DeSantis and J. Bryan Jones -- Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product / Xueni Chen [and others] -- Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA / Jesper Brons-Poulson, Jane Nohr, and Leif Kongskov Larsen -- Generation of Epitope-Tagged Proteins by Inverse Polymerase Chain Reaction Mutagenesis / Lucio Gama and Gerda E. Breitwieser -- Site-Directed Mutagenesis by Polymerase Chain Reaction / Albert Jeltsch and Thomas Lanio -- Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction / Amom Ruhikanta Meetei and M.R.S. Rao -- Phenotypic Expression of Polymerase Chain Reaction-Generated Random Mutations in a Foreign Gene After its Introduction into an Acinetobacter Chromosome by Natural Transformation / David M. Young, Ruben G. Kok, and L. Nicholas Ornston -- Polymerase Chain Reaction-Mediated Mutagenesis in Sequences Resistant to Homogeneous Amplification / Ross N. Nazar, P.D. Abeyrathne, and Robert V.A. Intine -- Polymerase Chain Reaction-Based Signature-Tagged Mutagenesis / Dario E. Lehoux and Roger C. Levesque -- High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction / Stefan Howorka and Hagan Bayley -- In Vitro Scanning-Saturation Mutagenesis / Jennifer A. Maynard [and others] -- Random Transposon Mutagenesis of Large DNA Molecules in Escherichia coli / Wolfram Brune -- Random Chromosomal Gene Disruption Using Cassette Mutagenesis / French A. Lewis, III and Brian Dougherty -- Transplacement Mutagenesis : A Recombination-Based In Situ Mutagenesis Protocol / Knut Woltjen, M.W. Todd Unger, and Derrick E. Rancourt -- Preparation of Transposon Insertion Lines and Determination of Insertion Sites in Arabidopsis Genome / Takuya Ito, Reiko Motohashi, and Kazuo Shinozaki -- Evolutionary Molecular Engineering by Random Elongation Mutagenesis / Tomoaki Matsuura, Tetsuya Yomo, and Itaru Urabe -- Random Mutagenesis for Protein Breeding / Chris Fenton [and others] -- DNA Shuffling and Family Shuffling for In Vitro Gene Evolution / Miho Kikuchi and Shigeaki Harayama -- Mutagenic Polymerase Chain Reaction of Protein-Coding Genes for In Vitro Evolution / Ichiro Matsumura and Andrew D. Ellington.

Print version record.

In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventional site-directed mutagenesis, with chapters being devoted to chemical site-directed mutagenesis; PCR-based mutagenesis and the modifications that allow high-throughput experiments; and mutagenesis based on gene disruption that is both in vitro- and in situ-based. Additional methods are provided for in vitro gene evolution; for gene disruption based on transposon, recombination, and cassette mutagenesis; and for facilitating the introduction of multiple mutations. Each readily reproducible technique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers. Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today's molecular biologists a rich compendium of reliable and powerful techniques with which to illuminate the proteome.

English.