Renal disease : techniques and protocols / edited by Michael S. Goligorsky.

Includes bibliographical references and index.

Standards of animal care in biological experiments / E.M. Levee -- Models of polycystic kidney disease / P. Upadhya -- Rat models of the metabolic syndrome / S.P. Tofovic and E.K. Jackson -- Models of glomerulonephritis / R.V. Durvasula and S.J. Shankland -- Functional studies of the kidney with magnetic resonance imaging / H.B. Marcos, Y. Zhang and P.L. Choyke -- Use of radionuclides to study renal function / Y. Liu and M.D. Blaufox -- Intravital videomicroscopy / T. Yamamoto and F. Kajiya -- Confocal and two-photon microscopy / J. Peti-Peterdi and P.D. Bell -- Atomic force microscopy in renal physiology / R.M. Henderson -- Freeze-fracture analysis of renal-epithelial tight junctions / H. Sasaki -- Organ culture of intact metanephric kidneys / S.J. Welham and A.S. Woolf -- The ureteric bud. Tissue-culture approaches to branching morphogenesis and inductive signaling / A.O. Perantoni -- Studies of cell lineage in the developing kidney / M.L. Sequeira Lopez and R.A. Gomez -- Transient transfection assays for analysis of signal transduction in renal cells / R.L. Maser [and others] -- The study of gene polymorphisms. How complex is complex genetic disease? / S. Bahring, A. Aydin and F.C. Luft -- Laser-capture microdissection / L. Barisoni and R.A. Star -- Serial analysis of gene expression / M.A. el-Meanawy [and others] -- RNA labeling and hybridization of DNA microarrays / E.P. Bottinger, A. Novetsky and J. Zavadil -- Gene-expression analysis of microdissected renal biopsies / C.D. Cohen and M. Kretzler -- The use of SELDI ProteinChip array technology in renal disease research / E. Fung [and others] -- Clearance studies in genetically altered mice / W.T. Noonan and J.N. Lorenz -- Long-term blood-pressure monitoring in unrestrained animals / K.A. Griffin, I. Abu-Amarah and A.K. Bidani -- In vitro studies on renin release / B.L. Jensen, U.G. Friis and O. Skott -- Mitochondrial function / J.M. Weinberg and P. Saikumar -- Detection of cysteine S-nitrosylation and tyrosine 3-nitration in kidney proteins / M. Crabtree, G. Hao and S.S. Gross -- Products of arachidonic acid metabolism / M.A. Carroll, J.C. McGiff and N.R. Ferreri -- Methods for measurements of heme oxygenase (HO) isoforms-mediated synthesis of carbon monoxide and HO-1 and HO-2 proteins / N.G. Abraham [and others] -- The juxtamedullary nephron preparation / D. Casellas and L.C. Moore -- Tubuloglomerular feedback / V. Vallon and J. Schnermann -- Microdissected perfused vessels / T.L. Pallone -- Renal microperfusion techniques / C.S. Wingo, I.D. Weiner and S.L. Xia -- How to design a clinical trial / B.M. Curtis, B.J. Barrett and P.S. Parfrey -- Mapping the disease / M.S. Goligorsky.

Print version record.

Today's investigator, overwhelmed by the information avalanche, may find it difficult to analyze emerging advances in technology. In Renal Disease: Techniques and Protocols, leading experts distill their experience into user-friendly methods for studying fluid-electrolyte homeostasis and kidney function in health and disease. Described in step-by-step detail, the techniques move from the molecular level to the whole organism, and from simple models to integrative physiology. Here the researcher will find established disease models, methods for optimizing renal disease research, as well as much help in choosing imaging techniques for studies of the kidney's structure and function in health, disease, and during embryonic development. Subjects covered include molecular diagnostics, strategies for studying the molecular mechanisms of kidney disease, technical means to assess the functional correlates of disease, and the planning of clinical trials. In addition to detailed instructions, each cutting-edge method includes troubleshooting advice, lists of reagents, and tips on avoiding pitfalls. State-of-the-art and highly practical, Renal Disease: Techniques and Protocols is not only a vade mecum for the many techniques employed to study the kidney in health and disease today, but also a user-friendly guide for the uninitiated, and a thoughtful consult for the experienced researcher.

English.