Nitric oxide protocols.

Includes bibliographical references and index.

Measurement of nitric oxide by reconversion of nitrate/nitrite to NO / Reinhard Berkels, Svenja Purol-Schnabel, and Renate Roesen -- High-resolution capillary electrophoresis of nitrite and nitrate in biological samples / Dmitri Y. Boudko -- Nitrite and nitrate measurements in human urine by capillary electrophoresis / Edward Morcos and N. Peter Wiklund -- In vivo nitric oxide measurements using a microaxial electrode / Yoshiichiro Kitamura [and others] -- Measurement of exhaled nitric oxide / Peter A. Steerenberg and Jan G.C. van Amsterdam -- Nitric oxide imaging in neurons using confocal microscopy / Xiaoxiang Zheng [and others] -- Measurement of nitric oxide synthase activity in vivo and in vitro by gas -- Chromatography-mass spectroscopy / Dimitrios Tsikas.

A nonradioactive assay for nitric oxide synthase activity in tissue extracts / Hazel Sutherland [and others] -- Measurement of nitric oxide-related enzymes in the brain by in situ hybridization / Olivier Braissant -- Real-time polymerase chain reaction to quantify mRNA for endothelial nitric oxide synthase / Yi Chu and Frank M. Faraci -- Dioxygen-dependent metabolism of nitric oxide / Paul R. Gardner, Anne M. Gardner, and Craig K. Hallstrom -- Proteomic method for identification of tyrosine nitrated proteins / Kulwant S. Aulak [and others] -- Immunohistochemical detection of S-nitrosylated proteins / Andrew J. Gow [and others] -- Gene therapy for the delivery of endothelial nitric oxide synthase (eNOS) to the corpus cavernosum for erectile dysfunction / Trinity J. Bivalacqua [and others].

Adenovirus-mediated nitric oxide synthase gene transfer into the nucleus tractus solitarius in conscious rats / Yoshitaka Hirooka and Koji Sakai -- Gene therapy in cancer via use of a retrovector having a tumor specificity and expressing inducible nitric oxide synthase / Masahide Kuroki -- Gene transfer of nitric oxide synthase via the use of adeno-associated virus vectors / Yoshikazu Maeda, Kazuyuki Shimada, and Uichi Ikeda -- Adenovirus-mediated nitric oxide synthase gene transfer / Kathleen G. Raman [and others].

Print version record.

Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.

English.

Available to OhioLINK libraries.