Antifungal agents : methods and protocols / edited by Erika J. Ernst and P. David Rogers.

Includes bibliographical references and index.

Susceptibility testing methods of antifungal agents / Erika J. Ernst -- DNA fingerprinting methods for Candida species / Shawn R. Lockhart [and others] -- CARE-2 fingerprinting of Candida albicans isolates / Joachim Morschhuser, Peter Staib, and Gerwald Kuhler -- Targeted gene deletion in Candida albicans wild-type strains by MPAR flipping / Joachim Morschhuser, Peter Staib, and Gerwald Kuhler -- Application of DNA microarray analysis to the study of azole antifungal resistance in Candida albicans / Katherine S. Barker and P. David Rogers -- Application of proteomic analysis to the study of azole antifungal resistance in Candida albicans / Massoumeh Z. Hooshdaran, George M. Hilliard, and P. David Rogers -- Techniques for antifungal susceptibility testing of Candida albicans biofilms / Gordon Ramage and Jose Luis Lopez-Ribot -- Natural products and antifungal drug discovery / Melissa R. Jacob and Larry A. Walker -- Animal model of disseminated candidiasis / David Andes -- Murine model of invasive aspergillosis / Russell E. Lewis and Nathan P. Wiederhold -- Antifungal combinations / Roxana G. Vitale, Javier Afeltra, and Eric Dannaoui -- Post-antifungal effect methods / Roxana G. Vitale -- Evaluation of immunotherapy in invasive candidiasis: antifungal activity and cytokine expression assays / Maria Simitsopoulou and Emmanuel Roilides -- Interaction of histoplasma capsulatum with human macrophages, dendritic cells, and neutrophils / Simon L. Newman -- Immunology of infection owing to cryptococcus neoformans / Floyd L. Wormley, Jr. and John R. Perfect.

Print version record.

With the growing frequency of fungal infections even as resistance to existing antifungal agents increases, methods for finding novel drug targets and evaluating antifungal resistance have become more important than ever. In Antifungal Agents: Methods and Protocols, expert scientists describe in detail the state-of-the-art molecular methods they have optimized for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. Many of the protocols were developed in the laboratory and, when a method is limited to specific organisms, include alternative techniques to expand their application. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists, and molecular biologists the productive tools they need today in order to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.