Human airway inflammation : sampling techniques and analytical protocols / edited by Duncan F. Rogers and Louise E. Donnelly.

Includes bibliographical references and index.

Airway inflammation and remodeling in asthma / S.T. Holgate -- Biopsy techniques / M. Saetta and G. Turato -- Bronchoalveolar lavage (BAL) / C. Ward and E.H. Walters -- Nasal secretions and exudations / L. Greiff, M. Andersson and C.G.A. Persson -- Induced sputum / M.M. Kelly, A. Efthimiadis and F.E. Hargreave -- Induced sputum / V.M. Keatings and J.A. Nightingale -- Measurement of exhaled nitric oxide and carbon monoxide / S.A. Kharitonov -- Measurement of exhaled hydrocarbons / P. Paredi, S.A. Kharitonov and P.J. Barnes -- Breath condensate as a vehicle for collection of inflammatory mediators, especially hydrogen peroxide / J.C. Jongste, R. Jöbsis and H. Rolien Raatgeep -- Airway epithelial cells (primaries vs cell lines) / L.E. Donnelly -- Isolation and culture of human alveolar type II pneumocytes / I.R. Witherden and T.D. Tetley -- Pulmonary artery endothelial cells / J. Seybold and N. Suttorp -- Isolation and culture of human airway smooth muscle cells / R.A. Panettieri -- Isolation and purification of human mast cells and basophils / B.F. Gibbs and M. Ennis -- Neutrophils / S.V. Culpitt -- Macrophages / L.W. Poulter and C.M. Burke -- Collection, separation, and activation of human T lymphocytes / Y. Sotsios and S.G. Ward -- Eosinophils / L. Koenderman [and others] -- Analysis of gene expression / E.-B. Haddad and J. Rousell -- Analysis of gene expression / J. Rousell and E.-B. Haddad -- The technique of in situ hybridization / S. Ying and A. Barry Kay -- Measurement of airway mucin gene expression / K. Pritchard, A.K. Smith and D.F. Rogers -- Transcription factors and inflammatory gene regulation / R. Newton and K.J. Staples -- Inflammatory mediators in spontaneously produced sputum / A. Hill [and others] -- Flow cytometry / A. Hartnell -- Measurement of granulocyte pharmacodynamics in whole blood by flow cytometry / S.A. Bryan [and others] -- Current in vitro models of leukocyte migration / J.R. Allport [and others] -- Tracing intracellular mediator storage and mobilization in eosinophils / S. Mahmudi-Azer, P. Lacy and R. Moqbel -- Measurement of metalloproteinases / S.D. Shapiro, D. Kelley and D. Kobayashi -- Immunocytochemistry and immunohistochemistry / J. Springer and A. Fischer -- The measurement of cysteinyl leukotrienes in urine / J.Y. Westcott and S.E. Wenzel -- Quantitative analysis of cyclooxygenase metabolites of arachidonic acid / R. Dworski, J.R. Sheller and B.W. Christman -- Quantitative analysis of F2 isoprostanes / R. Dworski, B.W. Christman and J.R. Sheller.

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Airway inflammation is now recognized as central to the pathophysiology of several major respiratory conditions-the two most common being asthma and chronic obstructive pulmonary disease (COPD). In Human Airway Inflammation: Sampling Techniques and Analytical Protocols, experienced basic and clinical investigators describe a wide range of readily reproducible methods for sampling, isolating, and culturing all of the major inflammatory cells involved in the pathophysiology of asthma and COPD. The collection techniques detailed here involve biopsy and sampling of airway liquids such as sputum and exhaled gases such as nitric oxide. Also provided is a full range of methods for the isolation and characterization of cells and for the measurement of many of the major inflammatory markers, along with protocols for measuring the inflammatory mediators and enzymes released during lung inflammation. Each user-friendly method contains all the essential step-by-step elements the researcher must navigate from sample collection to analysis, and includes notes on how to avoid problems. Among the more advanced techniques are the measurement of exhaled hydrocarbons and F2 isoprostanes, granulocyte pharmacodynamics in whole blood measured by flow cytometry, and tracing mediator trafficking in eosinophils using confocal microscopy. Comprehensive and highly practical, the methods presented in Human Airway Inflammation: Sampling Techniques and Analytical Protocols provide today's basic and clinical researchers with all the major techniques for investigating airway inflammation, and powerfully illuminate many novel targets for emerging drugs.

English.