Immunochemical protocols / edited by Margaret M. Manson.

Includes bibliographical references and index.

Production of polyclonal antisera -- Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogens -- Raising polyclonal antibodies using nitrocellulose-bound antigen -- Synthesis of peptides for use as immunogens -- Production and characterization of antibodies against synthetic peptides -- Preparation and testing of monoclonal antibodies to recombinant proteins -- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase -- Purification of immunoglobulin G (IgG) -- Epitope mapping -- Enzyme-antienzyme method for immunohistochemistry -- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates -- Immunohistochemical detection of bromodeoxyuriding-labeled nuclei for in vivo cell kinetic studies -- Avidin-biotin technology: preparation of biotinylated probes -- Avidin-biotin technology: preparation of avidin conjugates -- Immunochemical applications of avidin-biotin technology -- Preparation of gold probes -- Immunogold probes for light microscopy -- Immunogold probes in electron microscopy -- Electron microscopic silver enhancement for double labeling with antibodies raised in the same species -- Quantitative and qualitative immunoelectrophoresis -- Rocket immunoelectrophoresis techniques or electroimmunodiffusion -- Crossed immunoelectrophoresis -- Crossed immunoaffinoelectrophoresis -- Immunodetection of proteins by western blotting -- Erasable western blots -- Colloidal gold staining and immunoprobing on the same western blot -- Colloidal gold staining and immunodetection in 2-D protein mapping -- Fluorescent protein staining on nitrocellulose with subsequent immunodetection of antigen -- Competitive ELISA -- Twin-site ELISAs for fos and myc oncoproteins using the AMPAK system -- Preparation of cytotoxic antibody-toxin conjugates -- Immunoaffinity purification and quantification of antibody-toxin conjugates -- An immuno-slot-blot assay for detection and detection and quantitation of alkyldeoxyguanosines in DNA -- Production of monoclonal antibodies for the detection of chemically modified DNA -- Sensitive competitive enzyme-linked immunoassay for quantitation of modified bases in DNA -- An immunochemical assay for detecting transition of B-DNA to Z-DNA -- Cell sorting using immunomagnetic beads -- Cell preparation for flow cytometry -- Preparation of rat lung cells for flow cytometry -- The isolation of rat hepatocytes for flow cytometry -- Flow cytometric analysis of cells using an antibody to a surface antigen -- Multiple immunofluorescence analysis of cells using flow cytometry -- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine -- Production and use on nonradioactive hybridization probes -- Cellular human and viral DNA detection by nonisotopic in situ hybridization -- Chromosomal mapping of genes by nonisotopic in situ hybridization -- Nonisotopic in situ hybridization -- Biotinylated probes in colony hybridization -- Screening of agt11 cDNA libraries using monoclonal antibodies -- Expression of foreign genes in mammalian cells using an antibody fusion system.

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Highlights recent advances in immunochemical techniques and their impact on basic research and clinical medicine, combining the forces of experts in a wide range of subspecialties to cover a wealth of information.

English.