Molecular methods in developmental biology : Xenopus and zebrafish / edited by Matthew Guille.

Includes bibliographical references and index.

The animal cap assay / Jeremy Green -- Cell and tissue transplantation in zebrafish embryos / Toshiro Mizuno, Minori Shinya, and Hiroyuki Takeda -- Ribonuclease protection analysis of gene expression in Xenopus / Craig S. Newman and Paul A. Krieg -- Quantitative analysis of mRNA levels in Xenopus embryos by reverse transcriptase-polymerase chain reaction (RT-PCR) / Oliver C. Steinbach and Ralph A.W. Rupp -- Wholemount in situ hybridization of Xenopus and zebrafish embryos / Joanne Broadbent and E. Mary Read -- In situ hybridization to sections of Xenopus embryos / David Bertwistle -- Zebrafish immunohistochemistry / Rachel Macdonald -- Immunohistochemistry of Xenopus embryos / Carl Robinson and Matt Guille -- Preparation and testing of synthetic mRNA for microinjection / Wendy Moore and Matt Guille -- Microinjection into Xenopus oocytes and embryos / Matt Guille -- Microinjection into zebrafish embryos / Qiling Xu -- Expression from DNA injected into Xenopus embryos / Ondine Cleaver and Paul A. Krieg -- Promoter analysis in zebrafish embryos / Jos Joore -- Transient transgenesis in Xenopus laevis facilitated by AAV-ITRs / Yuchang Fu, Donghui Kan, and Sylvia Evans -- Band-shift analysis using crude oocyte and embryo extracts from Xenopus laevis / Rob Orford and Matt Guille -- DNA-footprinting using crude embryonic extracts from Xenopus laevis / Rob Orford, Darren Mernagh, and Matt Guille -- Mapping protein-DNA interactions using in vivo footprinting / David Warshawsky and Leo Miller.

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In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.