Developmental biology protocols : Volume 1 / edited by Rocky S. Tuan and Cecilia W. Lo.

Includes bibliographical references and index.

Developmental biology protocols. Overview i / R.S. Tuan and C.W. Lo -- Rearing larvae of sea urchins and sea stars for developmental studies / C.J. Lowe and G.A. Wray -- Large-scale culture and preparation of sea urchin embryos for isolation of transcriptional regulatory proteins / J.A. Coffman and P.S. Leahy -- The chick embryo as a model system for analyzing mechanisms of development / D.K. Darnell and G.C. Schoenwolf -- Culture of avian embryos / D.K. Darnell and G.C. Schoenwolf -- Exo ovo culture of avian embryos / T. Ono -- Culture of preimplantation mouse embryos / A.S. Doherty and R.M. Schultz -- In vitro culture of rodent embryos during the early postimplantation period / M. Fujinaga -- Cryopreservation of mouse embryos / J. Richa -- Studying head and brain development in drosophila / R. Finkelstein -- Bioassays of inductive interactions in amphibian development / T. Ariizumi [and others] -- Gastrulation and early mesodermal patterning in vertebrates / G.C. Schoenwolf and J.L. Smith -- Craniofacial development of avian and rodent embryos / B.K. Hall and T. Miyake -- Examination of the axial skeleton of fetal rodents / M.G. Narotsky and J.M. Rogers -- Cardiac morphogenesis and dysmorphogenesis. An immunohistochemical approach / B.R. Waller, 3rd and A. Wessels -- Application of plastic embedding for sectioning whole-mount immunostained early vertebrate embryos / K.K. Linask and T. Tsuda -- Confocal microscopy of live xenopus oocytes, eggs, and embryos / C.A. Larabell -- Whole-mount immunolabeling of embryos by microinjection. Increased detection levels of extracellular and cell surface epitopes / C.D. Little and C.J. Drake -- Confocal laser scanning microscopy of morphology and apoptosis in organogenesis-stage mouse embryos / R.M. Zucker, E.S. Hunter, 3rd and J.M. Rogers -- Embryo/fetal topographical analysis by fluorescence microscopy and confocal laser scanning microscopy / R.M. Zucker and J.M. Rogers -- Magnetic resonance imaging analysis of embryos / B.R. Smith -- Optical coherence tomography imaging in developmental biology / S.A. Boppart, M.E. Brezinski and J.G. Fujimoto -- Ultrasound backscatter microscopy of mouse embryos / D.H. Turnbull -- Use of doppler echocardiography to monitor embryonic mouse heart function / K.K. Linask and J.C. Huhta -- Calcium imaging in cell-cell signaling / D.C. Slusarski and V.G. Corces -- Acquisition, display, and analysis of digital three-dimensional time-lapse (four-dimensional) data sets using free software applications / C.F. Thomas and J.G. White -- Cell lineage analysis. Applications of green fluorescent protein / M. Zernicka-Goetz and J. Pines -- Cell lineage analysis. X-inactivation mosaics / S.S. Tan, L. Godinho and P.P. Tam -- Retroviral cell lineage analysis in the developing chick heart / R.G. Gourdie [and others] -- Dynamic labeling techniques for fate mapping, testing cell commitment, and following living cells in avian embryos / D.K. Darnell [and others] -- Cell lineage analysis. Videomicroscopy techniques / P.J. Heid and J. Hardin -- Cell lineage analysis in xenopus embryos / S.A. Moody -- Photoactivatable (caged) fluorescein as a cell tracer for fate mapping in the zebrafish embryo / D.J. Kozlowski and E.S. Weinberg -- Carboxyfluorescein as a marker at both light and electron microscope levels to follow cell lineage in the embryo / D. Sun, C.M. Griffith and E.D. Hay -- Transplantation chimeras. Use in analyzing mechanisms of avian development / D.K. Darnell and G.C. Schoenwolf -- Interspecific chimeras in avian embryos / N.M. Le Douarin [and others] -- Quail-chick transplantation in the embryonic limb bud / E.E. LeClair and R.S. Tuan -- Mouse chimeras and the analysis of development / R.L. Gardner and T.J. Davies -- Cell grafting and fate mapping of the early-somite-stage mouse embryo / S.J. Kinder, S.S. Tan and P.P. Tam -- Interspecific chimeras. Transplantation of neural crest between mouse and chick embryos / M.L. Kirby [and others] -- Interspecific mouse-chick chimeras / J. Fontaine-Perus -- Mosaic analysis in caenorhabditis elegans / J. Yochem, M. Sundaram and E.A. Bucher -- Local application of bone morphogenic protein on developing chick embryos using fibrous glass matrix as a carrier / A. Nifuji and M. Noda -- Laser ablation and fate mapping / M.L. Kirby [and others] -- Photoablation of cells expressing beta-galactosidase / S. Nirenberg -- Exo utero surgery / V. Ngo-Muller and K. Muneoka -- Methods for constructing and producing retroviral vectors / A. Gambotto [and others] -- Retroviral gene transduction in limb bud micromass cultures / N.S. Stott and C.M. Chuong -- Construction of adenoviral vectors / A.R. Davis [and others] -- Application of adenoviral vectors. Analysis of eye development / J. Bennett [and others] -- The application of adenoviral vectors in the study of mammalian cardiovascular development / C.S. Mickanin and H.S. Baldwin.

Print version record.

Developmental Biology Protocols grows out of the need for a comprehensive laboratory manual that provides the readers the principles, background, rationale, as well as the practical protocols, for studying and analyzing the events of embryonic development.

This three-volume set, consisting of 142 chapters, is intentionally broad in scope, because of the nature of modern developmental biology.

Information is grouped into the following topics: (1) systems--production, culture, and storage; (2) developmental pattern and morphogenesis; (3) embryo structure and function; (4) cell lineage analysis; (5) chimeras; (6) experimental manipulation of embryos; (7) application of viral vectors; (8) organogenesis; (9) abnormal development and teratology;(10) screening and apping of novel genes and mutations; (11) trans genes is production and gene knockout; (12) manipulation of developmental gene expression and function; (13) analysis of gene expression; (14) models of morphogenesis and development; and (15) in vitro models and analysis of differentiation and development.