Cancer cell signaling : methods and protocols / edited by David M. Terrian.

Includes bibliographical references and index.

pt. 1. Manipulation and detection of survival signals -- Functional analysis of the antimitogenic activity of tumor supressors / Erik S. Knudsen -- Rescue and isolation of Rb-deficient prostate epithelium by tissue recombination / Simon W. Hayward -- Signal transduction study using gene-targeted embryonic stem cells / Hideki Kawasome -- The use of the yeast two-hybrid system to measure protein-protein interactions that occur following oxidative stress / Richard A. Franklin -- Differential screening of cDNA libraries for analysis of gene expression during tumor progression / Christopher W. Gregory -- Mitogen-activated protein kinase signaling in drug-resistant neuroblastoma cells / Raymond R. Mattingly -- TUNEL and immunofluorescence double-labeling assay for apoptotic cells with specific antigen(s) / Stephanie M. Overhaus -- pt. 2. Manipulation and detection of oncogenic signals -- Kinetworks protein kinase multiblot analysis / Steven Pelech -- Protein tyrosine kinase and phosphotase expression profiling in human cancers / Wen-Chang Lin -- Association of nonreceptor tyrosine kinase c-yes with tight junction protein occludin by coimmunoprecipitation assay / Yan-Hua Chen -- Isolation of novel substrates using a tyrosine kinase overlay/in situ assay / Irwin H. Gelman -- Manipulating expression of endogenous oncogenic proteins using an antisense oligonucleotide approach in prostate cancer cells / Daqing Wu -- Measurements of phospholipidases A2, C, and D (PLA2, PLC, and PLD): in vitro microassays, analysis of enzyme isoforms, and intact-cell assays / Julian Gomez-Camnronero -- Detection of the content and activity of the transcription factor AP-1 in a mutlistage skin carcinogenesis model / Yunfeng Zhao -- Fibroblastic, hematopoietic, and hormone responsive epithelial cell lines and culture conditions for elucidation of signal transduction and drug resistance pathways by gene transfer / Linda S. Steelman -- Elucidation of signal transduction pathways by transfection of cells with modified oncogenes / Linda S. Steelman -- Elucidation of signal transduction pathways by retroviral infection of cells with modified organisms / Linda S. Steelman -- pt. 3. Protein interactions -- Methods for the study of protein-protein interactions in cancer cell biology / Daniel Price -- Production of ligand-specific mutants using a yeast two-hybrid mating assay / André Nantel -- Coimmunoprecipitation assay for the detection of kinase-substrate interactions / Lucio Comai -- pt. 4. Genomic rearrangements -- Mutational analysis of the androgen receptor using laser capture microdissection and direct sequencing / Sheila Green -- Clonality analysis by T-cell receptor y PCR and high-resolution electrophoresis in the diagnosis of cutaneous T-cell lymphoma (CTCL) / Ansgar Lukowsky.

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The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid. Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.

English.