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Pichia protocols / edited by James M. Cregg.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 389.Publication details: Totowa, N.J. : Humana Press, 2007.Edition: 2nd edDescription: 1 online resource (xi, 268 pages) : illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781588294296
  • 1588294293
  • 1597454567
  • 9781597454568
Subject(s): Genre/Form: Additional physical formats: Print version:: Pichia protocols.DDC classification:
  • 660.62 22
LOC classification:
  • TP248.27.Y43 .P53 2007
NLM classification:
  • W1
  • QW 25
Other classification:
  • TQ920
Online resources:
Contents:
Introduction : distinction between Pichia pastoris and other expression systems / James M. Cregg -- Vectors and strains for expression / Joan Lin-Cereghino and Geoff P. Lin-Cereghino -- DNA-mediated transformation / James M. Cregg -- Rational design and optimization of fed-batch and continuous fermentation / Wenhui Zhag, Mehmet Inan, and Michael M. Meagher -- Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1) / Mehmet Inan [and others] -- Purification of the N- and C-terminal subdomains of recombinant heavy chain fragment C of botulinum neurotoxin serotype C / Jicai Huang [and others] -- Rapid screening of chromatography resins for the purification of proteins / Sandra E. Ríos, Erin M. Giaccone, and Tillman U. Gerngross -- Characterization of O-linked saccharides on glycoproteins / Roger K. Bretthauer -- Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using glycoswitch plasmids / Wouter Vervecken [and others] -- N-linked glycan characterization of heterologous proteins / Huijan Li [and others] -- Heavy labeling of recombinant proteins / Eric Rodriguez -- Selenomethionine labeling of recombinant proteins / Anna M. Larsson and T. Alwyn Jones -- Selective isotopic labeling of recombinant proteins using amino acid auxotroph strains / James W. Whittaker -- Classical genetics / Ilya Tolstorukov and James M. Cregg -- Identification of pexophagy genes by restriction enzyme-mediated integration / Laura A. Schroder, Benjamin S. Glick, and William A. Dunn, Jr. -- Characterization of protein-protein interactions : application to the understanding of peroxisome biogenesis / Sebastien Leon [and others] -- Localization of proteins and organelles using fluorescence microscopy / Jean-Claude Farre [and others] -- Fluorescence microscopy and thin-section electron microscopy / Benjamin S. Glick.
Summary: The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Protocols Available
Total holds: 0

Previous edition: 1998.

Includes bibliographical references and index.

Title from title screen (viewed November 20, 2007).

Introduction : distinction between Pichia pastoris and other expression systems / James M. Cregg -- Vectors and strains for expression / Joan Lin-Cereghino and Geoff P. Lin-Cereghino -- DNA-mediated transformation / James M. Cregg -- Rational design and optimization of fed-batch and continuous fermentation / Wenhui Zhag, Mehmet Inan, and Michael M. Meagher -- Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1) / Mehmet Inan [and others] -- Purification of the N- and C-terminal subdomains of recombinant heavy chain fragment C of botulinum neurotoxin serotype C / Jicai Huang [and others] -- Rapid screening of chromatography resins for the purification of proteins / Sandra E. Ríos, Erin M. Giaccone, and Tillman U. Gerngross -- Characterization of O-linked saccharides on glycoproteins / Roger K. Bretthauer -- Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using glycoswitch plasmids / Wouter Vervecken [and others] -- N-linked glycan characterization of heterologous proteins / Huijan Li [and others] -- Heavy labeling of recombinant proteins / Eric Rodriguez -- Selenomethionine labeling of recombinant proteins / Anna M. Larsson and T. Alwyn Jones -- Selective isotopic labeling of recombinant proteins using amino acid auxotroph strains / James W. Whittaker -- Classical genetics / Ilya Tolstorukov and James M. Cregg -- Identification of pexophagy genes by restriction enzyme-mediated integration / Laura A. Schroder, Benjamin S. Glick, and William A. Dunn, Jr. -- Characterization of protein-protein interactions : application to the understanding of peroxisome biogenesis / Sebastien Leon [and others] -- Localization of proteins and organelles using fluorescence microscopy / Jean-Claude Farre [and others] -- Fluorescence microscopy and thin-section electron microscopy / Benjamin S. Glick.

The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system.

English.

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