Methods in membrane lipids / edited by Alex M. Dopico.

Title from title screen (viewed November 20, 2007).

Includes bibliographical references and index.

A glance at the structural and functional diversity of membrane lipids / A.M. Dopico and G.J. Tigyi -- Membrane lipid polymorphism: Relationship to bilayer properties and protein function / R.M. Epand -- Acrylodan-labeled intestinal fatty acid-binding protein to measure concentrations of unbound fatty acids / J.R. Simard, F. Kamp and J.A. Hamilton -- Measuring molecular order and orientation using coherent anti-stokes raman scattering microscopy / H.A. Rinia, G.W. Wurpel and M. Muller -- Preparation of oriented, fully hydrated lipid samples for structure determination using x-ray scattering / S.A. Tristram-Nagle -- Nuclear magnetic resonance investigation of oriented lipid membranes / O. Soubias and K. Gawrisch -- Molecular dynamics simulations as a complement to nuclear magnetic resonance and x-ray diffraction measurements / S.E. Feller -- Use of inverse theory algorithms in the analysis of biomembrane nmr data / E. Sternin -- Statistical thermodynamics through computer simulation to characterize phospholipid interactions in membranes / M. Mezei and P. Jedlovszky -- Fluorometric assay for detection of sterol oxidation in liposomal membranes / P.L. Chong and M. Olsher -- Fluorescence detection of signs of sterol superlattice formation in lipid membranes / P.L. Chong, B. Venegas and M. Olsher -- Differential scanning calorimetry in the study of lipid phase transitions in model and biological membranes: Practical considerations / R.N. Lewis, D.A. Mannock and R.N. McElhaney -- Pressure perturbation calorimetry / P.D. Heerklotz -- Fourier transform infrared spectroscopy in the study of lipid phase transitions in model and biological membranes: Practical considerations / R.N. Lewis and R.N. McElhaney -- Optical dynamometry to study phase transitions in lipid membranes / R. Dimova and B. Pouligny -- Fluorescence assays for measuring fatty acid binding and transport through membranes / K. Brunaldi [and others] -- Measurement of lateral diffusion rates in membranes by pulsed magnetic field gradient, magic angle spinning-proton nuclear magnetic resonance / K. Gawrisch and H.C. Gaede -- Using fluorescence recovery after photobleaching to measure lipid diffusion in membranes / C.W. Mullineaux and H. Kirchhoff -- Single-molecule fluorescence microscopy to determine phospholipid lateral diffusion / M.J. Murcia, S. Garg and C.A. Naumann -- Modeling 2d and 3d diffusion / M.J. Saxton -- Measurement of water and solute permeability by stopped-flow fluorimetry / J.C. Mathai and M.L. Zeidel -- Fluorescence microscopy to study pressure between lipids in giant unilamellar vesicles / A. Celli, C.Y. Lee and E. Gratton -- X-ray scattering and solid-state deuterium nuclear magnetic resonance probes of structural fluctuations in lipid membranes / H.I. Petrache and M.F. Brown -- Determination of lipid spontaneous curvature from x-ray examinations of inverted hexagonal phases / M.M. Kozlov -- Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity, and line tension / S.T. Hess [and others] -- Laser tweezer deformation of giant unilamellar vesicles / C. Poole and W. Losert -- Measurement of lipid forces by x-ray diffraction and osmotic stress / H.I. Petrache, D. Harries and V.A. Parsegian -- Micropipet aspiration for measuring elastic properties of lipid bilayers / M.L. Longo and H.V. Ly -- Langmuir films to determine lateral surface pressure on lipid segregation / A. Cruz and J. Perez-Gil -- Detergent and detergent-free methods to define lipid rafts and caveolae / R.S. Ostrom and X. Liu -- Near-field scanning optical microscopy to identify membrane microdomains / A. Ianoul and L.J. Johnston -- Fluorescence microscopy to study domains in supported lipid bilayers / J.M. Crane and L.K. Tamm -- Fluorescence resonance energy transfer to characterize cholesterol-induced domains / L.M. Loura and M. Prieto -- Lipid domains in supported lipid bilayer for atomic force microscopy / W.C. Lin [and others] -- Nuclear magnetic resonance structural studies of membrane proteins in micelles and bilayers / X.M. Gong [and others] -- Laurdan studies of membrane lipid-nicotinic acetylcholine receptor protein interactions / S.S. Antollini and F.J. Barrantes -- Single-molecule methods for monitoring changes in bilayer elastic properties / O.S. Andersen [and others] -- Ion-channel reconstitution / F.J. Morera [and others] -- The use of differential scanning calorimetry to study drug-membrane interactions / T.M. Mavromoustakos -- Atomic force microscopy to study interacting forces in phospholipid bilayers containing general anesthetics / Z.V. Leonenko, E. Finot and D.T. Cramb.

Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field.

English.