Electroporation protocols for microorganisms / edited by Jac A. Nickoloff.

Includes bibliographical references and index.

Electroporation theory : concepts and mechanisms / James C. Weaver -- Instrumentation / Gunter A. Hofmann -- Direct plasmid transfer between bacterial species and electrocuring / Helen L. Withers -- Transfer of episomal and integrated plasmids from Saccharomyces cerevisiae to Escherichia coli by electroporation / Laura Gunn, Jennifer Whelden, Jac A. Nickoloff -- Production of cDNA libraries by electroporation / Christian E. Gruber -- Electroporation of RNA into Saccharomyces cerevisiae / Daniel R. Gallie -- Electrofusion of yeast protoplasts / Herbert Weber, Hermann Berg -- Escherichia coli electrotransformation / Elizabeth M. Miller, Jac A. Nickoloff -- Electrotransformation in Salmonella / Kenneth E. Sanderson, P. Ronald MacLachlan, Andrew Hessel -- Electrotransformation of Pseudomonas / Jonathan J. Dennis, Pamela A. Sokol -- Electroporation of Xanthomonas / Teresa J. White, Carlos F. Gonzalez -- Transformation of Brucella species with suicide and broad host-range plasmids / John R. McQuiston, Gerhardt G. Schurig, Nammalwar Sriranganathan, Stephen M. Boyle -- Electroporation of Francisella tularensis / Gerald S. Baron, Svetlana V. Myltseva, Francis E. Nano -- A simple and rapid method for transformation of vibrio species by electroporation / Hajime Hamashima, Makoto Iwasaki, Taketoshi Arai -- Genetic transformation of Bacteroides spp. using electroporation / C. Jeffrey Smith -- Electrotransformation of Agrobacterium / Jhy-Jhu Lin -- Electroporation of Helicobacter pylori / Ellyn D. Segal -- Electrotransformation of Streptococci / Robert E. McLaughlin, Joseph J. Ferretti -- Transformation of Lactococcus by electroporation / Helge Holo, Ingolf F. Nes -- Transformation of Lactobacillus by electroporation / Thea W. Aukrust, May B. Brurberg, Ingolf F. Nes -- Electrotransformation of staphylococci / Jean C. Lee -- Electroporation and efficient transformation of Enterococcus faecalis grown in high concentrations of glycine / Brett D. Shepard, Michael S. Gilmore -- Introduction of recombinant DNA into Clostridium spp. / Mary K. Phillips-Jones -- Electroporation of mycobacteria / T. Parish, N.G. Stoker -- Electrotransformation of the spirochete Borrelia burgdorferi / D. Scott Samuels -- Yeast transformation and the preparation of frozen spheroplasts for electroporation / Lisa Stowers, James Gautsch, Richard Dana, Merl F. Hoekstra -- Ten-minute electrotransformation of Saccharomyces cerevisiae / Martin Grey, Martin Brendel -- Electroporation of Schizosaccharomyces pombe / Mark T. Hood, C.S. Stachow -- Gene transfer by electroporation of filamentous fungi / M. Kapoor -- Transformation of Candida maltosa by electroporation / Dietmar Becher, Stephen G. Oliver -- Electroporation of Physarum polycephalum / Timothy G. Burland, Juliet Bailey -- Electroporation of Dictyostelium discoideum / David Knecht, Ka Ming Pang -- Gene transfer by electroporation in Tetrahymena / Jacek Gaertig, Martin A. Gorovsky -- Transfection of the African and American trypanosomes / John M. Kelly, Martin C. Taylor, Gloria Rudenko, Pat A. Blundell -- Electroporation in Giardia lamblia / A.L. Wang, Tiina Sepp, C.C. Wang.

Print version record.

English.

Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro­ organisms describe the underlying theory of electroporation, the com­ mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre­ sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro­ duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ­ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.