Molecular bacteriology : protocols and clinical applications / edited by Neil Woodford and Alan P. Johnson.

Includes bibliographical references and index.

Microbiology and laboratory diagnosis / K. Cartwright-Isolation, culture, and identification of meningococci from clinical specimens / P. Olcen and H. Fredlund-Pcr diagnosis / M. Guiver and R. Borrow-Detection of meningococcal antigen by latex agglutination / M.A. Sobanski, R.A. Barnes and W.T. Coakley-Meningococcal serology / S.J. Gray, R. Borrow and E.B. Kaczmarski-Antibiotic susceptibility testing / C. Block-Molecular approach for the study of penicillin resistance in neisseria meningitidis / L. Arreaza and J.A. Vazquez-Overview: Epidemiology, surveillance, and population biology / M.C. Maiden and N.T. Begg-Serological characterization / B. Kuipers [and others]-Pulsed-field gel electrophoresis / M. Achtman and G. Morelli-Nucleotide sequencing of antigen genes of neisseria meningitidis / R. Urwin-Multi-locus sequence typing / K.A. Jolley-Capsular operons / U. Vogel, H. Claus and M. Frosch-Molecular analysis of the meningococcal lps expression / M.P. Jennings, A.W. Berrington and E.R. Moxon-Genome sequencing and annotation / A.C. Jeffries, N.J. Saunders and D.W. Hood-Representational difference analysis / L.D. Bowler, A. Bart and A. van der Ende-Managing outbreaks : The public health response / J.M. Stuart-Global trends in meningococcal disease / D.A. Caugant-Epidemiology, surveillance, and population biology : Carriage studies / K. Cartwright-Surveillance of meningococcal disease in europe / N. Noah-Global surveillance for meningococcal disease / N.E. Rosenstein-Epidemiology of meningococcal disease in north america / A.J. Pollard, D. Scheifele and N. Rosenstein-Computational methods for meningococcal population studies / K.A. Jolley and R. Urwin-Clinical studies : An overview / F.A. Riordan and A.J. Pollard-Clinical scoring systems in meningococcal disease / M. Festa and B. Derkx-Quantitative detection of bacterial lipopolysaccharides in clinical specimens / P. Brandtzaeg, R. Ovstebo and P. Kierulf-Isolation and analysis of radiolabeled meningococcal endotoxin / P.C. Giardina [and others]-Cytokine measurement in vivo and in vitro / M. van Deuren and P. Brandtzaeg-Cytokine bioassays / N.B. Liabakk [and others]-Molecular analysis of gene-polymorphisms affecting the host response to infection / R.G. Westendorp and T.W. Huizinga-Coagulation studies / J.A. Hazelzet, C.E. Hack and R. de Groot-Complement pathways and meningococcal disease : Diagnostic aspects / A.G. Sjoholm, L. Truedsson and J.C. Jensenius-Evaluation of new treatments for meningococcal disease / S. Nadel, I. Macintosh and M. Levin-Studies on the effect of neisserial porins on apoptosis of mammalian cells / P. Massari and L.M. Wetzler-Molecular recognition mechanisms of meningococci : Identifying receptor-ligand pairs involved in microbe-host interactions / M. Virji and L. Serino-Experimental nasopharyngeal colonization by neisseria meningitidis using explant organ culture / R.C. Read and L. Goodwin-Invasion of the central nervous system by neisseria meningitidis / C. Tinsley and X. Nassif-Interactions of meningococci with endothelium / R.S. Heyderman and N.J. Klein-A promoter probe plasmid based on green fluorescent protein : A strategy for studying meningococcal gene expression / S.A. Webb, P.R. Langford and J.S. Kroll-Signature-tagged mutagenesis / S. Bakshi [and others].

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In Molecular Bacteriology: Protocols and Clinical Applications, Woodford and Johnson bring together in a single volume a state-of-the-art collection of detailed molecular methods for the diagnosis and clinical investigation of bacterial infections. These wide ranging techniques-each presented by recognized experts who have fine-tuned their methods for optimum performance-will permit readers to establish them successfully in their own laboratories. An accompanying series of minireviews highlights and explains the clinical and epidemiological relevance of the techniques and shows how they may be applied to major pathogen species and various types of antibiotic resistance. Molecular Bacteriology: Protocols and Clinical Applications enlarges our current understanding of the role that molecular methodology can play in modern medical bacteriology, fully explaining both the practical and theoretical aspects of the cutting-edge methods that are increasingly finding diagnostic use in laboratories throughout the world. An authoritative laboratory resource for everyone working with infectious diseases today, this book's insightful reviews and detailed protocols will soon win many devotees.

English.