Plant virology protocols : from viral sequence to protein function / Gary D. Foster [and others].

Includes bibliographical references and index.

Plant-virus interactions / Peter Palukaitis, John P. Carr, and James E. Schoelz -- Role of capsid proteins / John F. Bol -- Role of plant virus movement proteins / Michael Taliansky, Lesley Torrance, and Natalia O. Kalinina -- Multiple roles of viral replication proteins in plant RNA virus replication / Peter D. Nagy and Judit Pogany -- Role of silencing suppressor proteins / József Burgyán -- Role of vector-transmission proteins / Véronique Ziegler-Graff and Véronique Brault -- In vivo analyses of viral RNA translation / William R. Staplin and W. Allen Miller -- In vitro analysis of translation enhancers / Aurélie M. Rakotondrafara and W. Allen Miller -- Identification of plant virus IRES / Sek-Man Wong, Dora Chin-Yen Koh, and Dingxiang Liu -- Analysis of geminivirus DNA replication by 2-D gel / Keith Saunders -- Begomoviruses : molecular cloning and identification of replication origin / Lilian H. Florentino [and others] -- Analysis of viroid replication / Ricardo Flores [and others] -- Biochemical analyses of the interactions between viral polymerases and RNAs / Young-Chan Kim and C. Cheng Kao -- In situ detection of plant viruses and virus-specific products / Andrew J. Maule and Zoltán Havelda -- Detection of siRNAs and miRNAs / Sakari Kauppinen and Zoltán Havelda -- Cloning of short interfering RNAs from virus-infected plants / Thien X. Ho [and others].

Solution structure probing of RNA structures / Marc R. Fabian and K. Andrew White -- RNA encapsidation assay / Padmanaban Annamalai and A.L.N. Rao -- Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus / K.S. Rajendran and Peter D. Nagy -- Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis / Raúl C. Gomila and Lee Gehrke -- Probing interactions between plant virus movement proteins and nucleic acids / Tzvi Tzfira and Vitaly Citovsky -- Movement profiles : a tool for quantitative analysis of cell-to-cell movement of plant viral movement proteins / Kateryna Trutnyeva, Pia Ruggenthaler, and Elisabeth Waigmann -- Analysis of siRNA-suppressor of gene silencing interactions / Lórant Lakatos and József Burgyán -- Phosphorylation analysis of plant viral proteins / Kristiina M. Mäkinen and Konstantin I. Ivanov -- Analysis of interactions between viral replicase proteins and plant intracellular membranes / Hélène Sanfaçon and Guangzhi Zhang -- Membrane and protein dynamics in virus-infected plant cells / Michael Goodin, Romit Chakrabarty, and Sharon Yelton -- Site-directed mutagenesis of whole viral genomes / Li Liu and George P. Lomonossoff -- Viral protein-nucleic acid interaction : south (north)-western blot / Huanting Liu -- Protein-protein interactions : the yeast two-hybrid system / Deyin Guo, Minna-Liisa Rajamäki, and Jari Valkonen -- NMR analysis of viral protein structures / Andrew J. Dingley, Inken Lorenzen, and Joachim Grötzinger -- Localization of viral proteins in plant cells : protein tagging / Sophie Haupt, Angelika Ziegler, and Lesley Torrance -- Construction of infectious clones for RNA viruses : TMV / Sean N. Chapman -- Construction of infectious cDNA clones for RNA viruses : turnip crinkle virus / Eugene V. Ryabov -- Construction of infectious clones for DNA viruses : mastreviruses / Margaret I. Boulton -- Construction of infectious clones of double-stranded DNA viruses of plants using citrus yellow mosaic virus as an example / Qi Huang and John S. Hartung.

Insertion of introns : a strategy to facilitate assembly of infectious full length clones / I. Elisabeth Johansen and Ole Søgaard Lund -- Analysis of cell-to-cell and long-distance movement of apple latent spherical virus in infected plants using green, cyan, and yellow fluorescent proteins / Tsubasa Takahashi and Nobuyuki Yoshikawa -- Agroinoculation : a simple procedure for systemic infection of plants with viruses / Zarir E. Vaghchhipawala and Kirankumar S. Mysore -- Geminivirus : biolistic inoculation and molecular diagnosis / Anésia A. Santos [and others] -- Expression microarrays in plant-virus interaction / Kristina Gruden [and others] -- Genome-wide screens for identification of host factors in viral replication / Tadas Panavas [and others] -- Phosphorylation of movement proteins by the plasmodesmal-associated protein kinase / Jung-Youn Lee -- Virus-induced gene silencing as a tool to identify host genes affecting viral pathogenicity / Xiaohong Zhu and S.P. Dinesh-Kumar -- Yeast two-hybrid assay to identify host-virus interactions / Stuart A. MacFarlane and Joachim F. Uhrig.

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This new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of Plant Virology Protocols presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies.

English.