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Live cell imaging : methods and protocols / edited by Dmitri B. Papkovsky.

Contributor(s): Material type: TextTextSeries: Springer protocols (Series) | Methods in molecular biology (Clifton, N.J.) ; v. 591.Publication details: New York, N.Y. : Humana Press, ©2010.Description: 1 online resource (xi, 367 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781607614043
  • 1607614049
Subject(s): Genre/Form: Additional physical formats: No titleDDC classification:
  • 579 23
LOC classification:
  • QH585
NLM classification:
  • QY 95
Online resources:
Contents:
Instrumentation for live-cell imaging and main formats / Martin Oheim -- Labels and probes for live cell imaging: overview and selection Guide / Scott A. Hilderbrand -- Live cell imaging: an industrial perspective / Terry McCann -- Design of fluorescent fusion protein probes / Elizabeth Pham, Kevin Truong -- Synthetic fluorescent probes for imaging of peroxynitrite and hypochlorous acid in living cells / Dan Yang, Zhen-Ning Sun, Tao Peng, Hua-Li Wang, Jian-Gang Shen, Yan Chen, Paul Kwong-Hang Tam -- Photo-activatable probes for the analysis of receptor function in living cells / Wen-Hong Li -- The Application of fluorescent probes for the analysis of lipid dynamics during phagocytosis / Ronald S. Flannagan, Sergio Grinstein -- Imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor / Kenji Sugimoto, Shigenobu Tone -- Manipulation of neutrophil-like HL-60 cells for the study of directed cell migration / Arthur Millius, Orion D. Weiner -- A Method for analyzing protein-protein interactions in the plasma membrane of live B cells by fluorescence resonance energy transfer imaging as acquired by total internal reflection fluorescence microscopy / Hae Won Sohn, Pavel Tolar, Joseph Brzostowski, Susan K. Pierce -- Sample preparation for STED microscopy / Christian A. Wurm, Daniel Neumann, Roman Schmidt, Alexander Egner, Stefan Jakobs -- Two-photon permeabilization and calcium measurements in cellular organelles / Oleg Gerasimenko, Julia Gerasimenko -- Imaging and analysis of three-dimensional cell culture models / Benedikt W. Graf, Stephen A. Boppart -- Long-term imaging in microfluidic devices / Gilles Charvin, Catherine Oikonomou, Frederick Cross -- Monitoring of cellular responses to hypoxia / Joachim Fandrey, Christoph Wotzlaw -- Imaging of cellular oxygen and analysis of metabolic responses of mammalian cells / Andreas Fercher, Tomas C. O'Riordan, Alexander V. Zhdanov, Ruslan I. Dmitriev, Dmitri B. Papkovsky / Martin vandeVen, Corina Balut, Szilvia Baron, Ilse Smets, Paul Steels, Marcel Ameloot -- Live cell imaging analysis of receptor function / Daniel C. Worth, Maddy Parsons -- Subcellular dynamic imaging of protein-protein interactions in live cells by bioluminescence resonance energy transfer / Julie Perroy -- Quantitative analysis of membrane potentials / Manus W. Ward -- Image correlation spectroscopy to define membrane dynamics / Jeremy Bonor, Anja Nohe.
Summary: Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.
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Total holds: 0

Title from PDF title page (SpringerLink, viewed December 7, 2009).

Includes bibliographical references and index.

Instrumentation for live-cell imaging and main formats / Martin Oheim -- Labels and probes for live cell imaging: overview and selection Guide / Scott A. Hilderbrand -- Live cell imaging: an industrial perspective / Terry McCann -- Design of fluorescent fusion protein probes / Elizabeth Pham, Kevin Truong -- Synthetic fluorescent probes for imaging of peroxynitrite and hypochlorous acid in living cells / Dan Yang, Zhen-Ning Sun, Tao Peng, Hua-Li Wang, Jian-Gang Shen, Yan Chen, Paul Kwong-Hang Tam -- Photo-activatable probes for the analysis of receptor function in living cells / Wen-Hong Li -- The Application of fluorescent probes for the analysis of lipid dynamics during phagocytosis / Ronald S. Flannagan, Sergio Grinstein -- Imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor / Kenji Sugimoto, Shigenobu Tone -- Manipulation of neutrophil-like HL-60 cells for the study of directed cell migration / Arthur Millius, Orion D. Weiner -- A Method for analyzing protein-protein interactions in the plasma membrane of live B cells by fluorescence resonance energy transfer imaging as acquired by total internal reflection fluorescence microscopy / Hae Won Sohn, Pavel Tolar, Joseph Brzostowski, Susan K. Pierce -- Sample preparation for STED microscopy / Christian A. Wurm, Daniel Neumann, Roman Schmidt, Alexander Egner, Stefan Jakobs -- Two-photon permeabilization and calcium measurements in cellular organelles / Oleg Gerasimenko, Julia Gerasimenko -- Imaging and analysis of three-dimensional cell culture models / Benedikt W. Graf, Stephen A. Boppart -- Long-term imaging in microfluidic devices / Gilles Charvin, Catherine Oikonomou, Frederick Cross -- Monitoring of cellular responses to hypoxia / Joachim Fandrey, Christoph Wotzlaw -- Imaging of cellular oxygen and analysis of metabolic responses of mammalian cells / Andreas Fercher, Tomas C. O'Riordan, Alexander V. Zhdanov, Ruslan I. Dmitriev, Dmitri B. Papkovsky / Martin vandeVen, Corina Balut, Szilvia Baron, Ilse Smets, Paul Steels, Marcel Ameloot -- Live cell imaging analysis of receptor function / Daniel C. Worth, Maddy Parsons -- Subcellular dynamic imaging of protein-protein interactions in live cells by bioluminescence resonance energy transfer / Julie Perroy -- Quantitative analysis of membrane potentials / Manus W. Ward -- Image correlation spectroscopy to define membrane dynamics / Jeremy Bonor, Anja Nohe.

Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.

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