Molecular diagnosis of infectious diseases / edited by Udo Reischl ; foreword by Hans Wolf.

Includes bibliographical references and index.

1. Recombinant proteins and peptides -- 2. Antibodies.

Identification of novel diagnostic markers by differential display / P. Liang [and others]-Immunoprecipitation / K. Johansen and L. Svensson-Rt-pcr methods and applications / P.D. Siebert-Quantitative rt-pcr / P.D. Siebert-Cloning cdna ends using race / Y. Zhang and M.A. Frohman-A method for the rapid identification of epitopes and other functional peptide domains / R.C. Mierendorf, B. Hammer and R.E. Novy-Mapping of linear b-cell epitopes on viral polypeptides by multiple peptide synthesis and fine tuning sensitivity and specificity of the identified peptide antigens for application in virus diagnosis / U. Wienhues [and others]-Amplification of genomic DNA by pcr / B. Frey-Pcr-based cloning and subsequent expression of antigenic proteins in escherichia coli / U. Reischl-Application of tev protease in protein production / D.A. Polayes [and others]-Stable transfected drosophila schneider-2 cells as a novel tool to produce recombinant antigens for diagnostic, therapeutic, and preventive purposes / L. Deml and R. Wagner-Use of recombinant antigens as diagnostic reagents in enzyme-linked immunosorbent assays / A. Warnes, A.R. Fooks and J.R. Stephenson-Generation of recombinant baculovirus DNA in e. Coli using a baculovirus shuttle vector / V.C. Ciccarone, D.A. Polayes and V.A. Luckow-The sfv gene expression system / V.C. Ciccarone, J.A. Jessee and P. Liljestrom-Expression and purification of recombinant proteins using the pet system / R.C. Mierendorf [and others]-Procedures for the analysis and purification of his-tagged proteins / R.E. Kneusel [and others]-Detection and immobilization of proteins containing the 6xhis tag / R.E. Kneusel, M. Wulbeck and J. Ribbe-The use of recombinant fusion proteases in the affinity purification of recombinant proteins / L.E. Leong-Purification and immunological characterization of recombinant antigens expressed in the form of insoluble aggregates (inclusion bodies) / U. Reischl-Purification of recombinant proteins having high isoelectric points / R.A. Calogero and A. Aulicino-Immunoblotting / M. Kirschfink and P. Terness-Diagnostic application of a multiple-channel immunoblot apparatus / S.D. Black-Immobilization of lmmunoreactants on solid phases for ellsas / R. Decker and D. Leahy-Nonradioactive labeling and lmmunodetection of recombinant proteins / R. Oberfelder-Flow cytometry / V. Daniel-Basic problems of serological laboratory diagnosis / W. Fierz-Molecular diagnostics resources on the internet / K.J. O'Donnell-Monoclonal antibody-based immunoassays / P. Appleby and U. Reischl-Biosensor-based methods in clinical diagnosis / F. Aberl and C. Kosslinger-The quartz crystal microbalance (qcm) as an immunosensor / F. Aberl, C. Kosslinger and H. Wolf-Biomolecular interaction analysis : Strategies for antibody and antigen characterization / U.W. Roder and F. Markey-Recent developments in antibody engineering / M. Little [and others]-Cloning and expression of single-chain fragments (scfv) from mouse and rat hybridomas / F. Breitling and S. Dubel-Production of a human antibody library in the phage-display vector psex81 / M. Welschof, M. Little and H. Dorsam-Screening of phage-displayed antibody libraries / H. Dorsam [and others]-Purification, characterization, and biotinylation of single-chain antibodies / S.M. Kipriyanov.

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In Molecular Diagnosis of Infectious Diseases leading international authorities provide a comprehensive and up-to-date set of basic and advanced methods that illuminate the principles of molecular diagnostics and allow researchers to establish assay systems tailored to their specific needs. This unique collection of protocols covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens, as well as their use as diagnostic reagents in immunological assays. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors. Molecular Diagnosis of Infectious Diseases offers clinical microbiologists, clinical chemists, and pathologists unique tools with which to develop improved assays for diagnosing infectious diseases. The book is certain to play a leading role in helping researchers to understand and realize in their daily work the powerful potential of molecular diagnostic techniques in clinical microbiology today.