Liposomes : methods and protocols / edited by Volkmar Weissig.

Includes bibliographical references and indexes.

v. 1. Pharmaceutical nanocarriers. Current trends in liposome research / Tamer A. ElBayoumi, Vladimir P. Torchilin. Nanoliposomes: preparation and analysis / M.R. Mozafari. Preparation of DRV liposomes / Sophia G. Antimisiaris. Elastic liposomes for topical and transdermal drug delivery / Heather A.E. Benson. Archaebacterial tetraetherlipid liposomes / Aybike Ozcetin, Samet Mutlu, Udo Bakowsky. Cationic magnetoliposomes / Marcel De Cuyper, Stefaan J.H. Soenen. Ultrasound-responsive liposomes / Shao-Ling Huang. Liposome formulations of hydrophobic drugs / Reto A. Schwendener, Herbert Schott. Remote loading of anthracyclines into liposomes / Felicitas Lewrick, Regine Peschka Suss. Arsonoliposomes: preparation and physicochemical characterization / Sophia G. Antimisiaris, Panayiotis V. Ioannou. Liposome-based vaccines / Reto A. Schwendener, Burkhard Ludewig, Andreas Cerny, Olivier Engler.-- Mannosylated liposomes for targeted vaccines delivery / Suresh Prasad Vyas, Amit K. Goyal, Kapil Khatri. Lipsomes for specific depletion of macrophages from organs and tissues / Nico van Rooijen, Esther Hendrickx. Vesicular phospholipid gels / Martin Brandl. Environment-responsive multifunctional lipsomes / Amit A. Kale, Vladimir P. Torchilin. Functional liposomes membranes for triggered release / Armagan Kocer. A "dock and lock" approach to preparation of targeted liposomes / Marina V. Backer, Joseph M. Backer. Conjunction of ligands to the surface of preformed liposomes by click chemistry / Benoit Frisch, Fatouma Said Hassane, Francis Schubert. Targeted magnetic liposomes loaded with doxornbicin / Pallab Pradhan, Rinti Banerjee, Dhirendra Bahadur, Christian Koch, Olga Mykhaylyk, Christian Plank. Liposomes for drug delivery to mitochondria / Sarathi V. Boddapati, Gerard G.M. D'Souza, Volkmar Weissig. Cytoskeletal-antigen specific immunoliposomes: preservation of myocardial viability / Vishwesh Patil, Tala Khudairi, Ban-An Khaw. Gadolinium-loaded polychelating polymer-containing tumor-targeted liposomes / Suna Erdogan, Vladimir P. Torchilin. Angiogenic vessel-targeting DDS by liposomalized oligopeptides / Tomohiro Asai, Naoto Oku. TAT-peptide modified liposomes: preparation, characterization, and cellular interaction / Marian M. Fretz, Gert Storm. ATP-loaded liposomes for targeted treatment in models of myocardial ischemia / Tatyana S. Levchenko, William C. Hartner, Daya D. Verma, Eugene A. Bernstein, Vladimir P. Torchilin. Intracellular ATP delivery using highly fusogenic liposomes / Sufan Chien. Lipoplex formation using liposomes prepared by ethanol injection / Yoshie Maitani. Acid-labile liposome/pDNA complexes / Michel Bessodes, Daniel Scherman. Serum-resistant lipoplexes in the presence of asialofetuin / Conchita Tros de ILarduya. Anionic pH sensitive lipoplexes / Nathalie Mignet, Daniel Scherman. Liposomal siRNA delivery / Keffrey Hughes, Preeti Yadava, Ryan Mesaros. Complexation of siRNA and pDNA with cationic liposomes: the important aspects in lipoplex preparation / Jose Mario Barichello, Tatsuhiro Ishida, Hiroshi Kiwada. Effective in vitro and in vivo gene delivery by the combination of liposomal bubbles (Bubble liposomes) and untrasound exposure / Ryo Suzuki, Kazuo Maruyama. Lipsomal magnetofection / Olga mykhaylyk, Yolanda Sanchez Antequera, Dialekti Vlashou, Edelburga Hammerschmid, Martina Anton, Olivier Zelphati, Christian Plank. Long-circulating, pH-sensitive liposomes / Denitsa Momekova, Stanislav Rangelov, Nikolay Lambov. Serum-stable, long-circulating, pH-sensitive PEGylated liposomes / Nicolas Bertrand, Pierre Simard, Jean-Christophe Leroux.

v. 2. Biological membrane models. Utilization of liposomes for studying drug transfer and uptake / Alfed Fahr and Xiangli Liu. The use of liposomes in the study of drug metabolism : a method to incorporate the enzymes of the cytochrome P450 monooxygenase system into phospholipid, bilayer vesicles / James R. Reed. Use of liposomes to study cellular osmosensors / Reinhard Krämer, Sascha Nicklisch, and Vera Ott. Studying mechanosensitive ion channels using liposomes / Boris Martinac [and others]. Studying amino acid transport using liposomes / Cesare Indiveri. Use of liposomes for studyng interactions of soluble proteins with cellular membranes / Chris T. Höfer, Andreas Herrmann, and Peter Müller. Liposomal reconstitution of monotopic integral membrane proteins / Zahra MirAfzali and David L. DeWitt. The reconstitution of actin polymerization on liposomes / Mark Stamnes and Weidong Xu. Electroformation of giant unilamellar vesicles from native membranes and organic lipid mixtures for the study of lipid domains under physiological ionic-strength conditions / L.-Ruth Montes [and others]. Visualization of lipid domain-specific protein sorting in giant unilamellar vesicles / Martin Stöckl, Jörg Nikolaus, and Andreas Herrmann. Biosynthesis of proteins inside liposomes / Pasquale Stano [and others]. Study of respiratory cytochromes in liposomes / Iseli L. Nantes [and others]. Use of liposomes to evaluate the role of membrane interactions on antioxidant activity / Salette Reis [and others]. Studying colloidal aggregation using liposomes / Juan Sabín, Gerardo Prieto, and Félix Sarmiento. Assessment of liposome-cell interactions / Jan A.A.M. Kamps. Methods to monitor liposome fusion, permeability, and interaction with cells / Nejat Düzgüneş, Henrique Faneca, and Maria C. Pedroso de Lima. The use of isothermal titration calorimetry to study multidrug transport proteins in liposomes / David Miller and Paula J. Booth. Studying lipid organization in biological membranes using liposomes and EPR spin labeling / Witold K. Subczynski, Marija Raguz, and Justyna Widomska. Membrane translocation assayed by fluorescence spectroscopy / J. Broecker and S. Keller. Interaction of lipids and ligands with nicotinic acetylcholine receptor vesicles assessed by electron paramagnetic resonance spectroscopy / Hugo Rubén Arias. Environmental scanning electron microscope imaging of vesicle systems / Yvonne Perrie [and others]. Freeze-fracture electron microscopy on domains in lipid mono- and bilayer on nano-resolution scale / Brigitte Papahadjopoulos-Sternberg. Atomic force microscopy for the characterization of proteoliposomes / Johannes Sitterberg [and others]. Method of simultaneous analysis of liposome components using HPTLC/FID / Sophia Hatziantoniou and Costas Demetzos. Viscometric analysis of DNA-lipid complexes / Sadao Hirota and Nejat Düzgüneş. Fluorometric analysis of individual cationic lipid-DNA complexes / Edwin Pozharski. Fluorescence resonance energy transfer-based analysis of lipoplexes / Edwin Pozharski. Analysis of lipoplex structure and lipid phase changes / Rumiana Koynova. Fluorescence methods for evaluating lipoplex-mediated gene delivery / Henrique Faneca, Nejat Düzgüneş, and Maria C. Pedroso de Lima. FRET imaging of cells transfected with siRNA/liposome complexes / Il-Han Kim [and others]. Spectral bio-imaging and confocal imaging of the intracellular distribution of lipoplexes / Sebastian Schneider and Regine Süss. Techniques for loading technetium-99m and rhenium-186/188 radionuclides into pre-formed liposomes for diagnostic imaging and radionuclide therapy / Beth Goins, Ande Bao, and William T. Phillips. Fluorescence correlation spectroscopy for the study of membrane dynamics and organization in giant unilamellar vesicles / Ana J. García-Sáez, Dolores C. Carrer, and Petra Schwille. Liposome biodistribution via europium complexes / Nathalie Mignet and Daniel Scherman. Biosensor-based evaluation of liposomal binding behavior / Gerd Bendas. Use of liposomes to study vesicular transport / Kohji Takei, Hiroshi Yamada, and Tadashi Abe.

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With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane.?Volume 1: Pharmaceutical Nanocarriers focuses on the state-of-the-art aspects of developing liposome-based nanoscale drug delivery techniques, delving into the depths of the emerging field of pharmaceutical and biomedical nanotechnology. As a volume in the highly successful Methods in Molecular BiologyTM series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.