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Antibody engineering. Volume 2 / Roland Kontermann, Stefan Dubel (eds.).

Contributor(s): Material type: TextTextSeries: Springer protocols (Series)Publication details: Berlin ; London : Springer, 2010.Edition: 2nd edDescription: 1 online resource (xii, 589 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9783642011474
  • 3642011470
  • 3642011462
  • 9783642011467
  • 1282830538
  • 9781282830530
  • 9786612830532
  • 6612830530
  • 9783642011481
  • 3642011489
  • 9783642422768
  • 3642422764
Subject(s): Genre/Form: Additional physical formats: Print version:: Antibody engineering. Volume 2.DDC classification:
  • 616.0798 22
LOC classification:
  • QR186.87 .A58 v.2
NLM classification:
  • QW 525
Online resources:
Contents:
Part I. Bioinformatics of Antigen-binding Sites -- 1. Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool / Svetlana Mollova, Ida Retter, Michael Hust, Stefan Dübel, and Werner Müller -- 2. Standardized Sequence and Structure Analysis of Antibody Using IMGT1 / François Ehrenmann, Patrice Duroux, Véronique Giudicelli, and Marie-Paule Lefranc -- 3. Protein Sequence and Structure Analysis of Antibody Variable Domains / Andrew C.R. Martin -- Part II. Generation of Antibody Fragments and Their Derivatives -- 4. scFv by Two-Step Cloning / Dafne Müller -- 5. Bivalent Diabodies / Roland E. Kontermann -- 6. Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies) / Tove Olafsen, Vania E. Kenanova, and Anna M. Wu -- 7. Miniantibodies / Jonas V. Schaefer, Peter Lindner, and Andreas Plückthun -- 8. Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins / Athanasios Mavratzas, Evelyn Exner, Jürgen Krauss, and Michaela A.E. Arndt -- 9. Antibody-Cytokine Fusion Proteins with Members of the TNF-Family / Dafne Müller and Jeannette Gerspach -- 10. Recombinant Immunotoxins for Treating Cancer / Ira Pastan and Mitchell Ho -- 11. T Bodies / Bianca Altvater, Silke Landmeier, and Claudia Rossig -- 12. Expressing Intrabodies in Mammalian Cells / Alessio Cardinale and Silvia Biocca -- 13. Phenotypic Knockdown with Intrabodies / Nina Strebe and Manuela Schüngel -- 14. Disulfide-Stabilized Fv Fragments / Ulrich Brinkmann -- 15. PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics / Arutselvan Natarajan and Sally J. DeNardo -- 16. Fusion Proteins with Improved PK / Roland Stork -- 17. In Vivo Biotinylated scFv Fragments / Laila Al-Halabi and Torsten Meyer -- 18. Bispecific Diabodies and Single-Chain Diabodies / Roland E. Kontermann -- 19. Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-IgTM) Molecule / Chengbin Wu, Tariq Ghayur, and Jochen Salfeld -- 20. Isolation of Antigen-Specific Nanobodies / Gholamreza Hassanzadeh Ghassabeh, Dirk Saerens, and Serge Muyldermans -- 21. CDR-FR Peptides / Xiao-Qing Qiu -- Part III. Production of Antibody Fragments -- 22. Purification and Characterization of His-Tagged Antibody Fragments / Martin Schlapschy, Markus Fiedler, and Arne Skerra -- 23. Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium / Miriam Steinwand, Eva Jordan, and Michael Hust -- 24. Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L / Remko Griep and John McDougall -- 25. Purification and Analysis of Strep-tagged Antibody-Fragments / Martin Schlapschy and Arne Skerra -- 26. Production of Antibodies and Antibody Fragments in Escherichia coli / Dorothea E. Reilly and Daniel G. Yansura -- 27. Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones / Jonas V. Schaefer and Andreas Plückthun -- 28. Bioreactor Production of scFv Fragments in Pichia pastoris / Stephan Hellwig and Georg Melmer -- 29. Expression of Antibody Fragments in Transgenic Plants / Udo Conrad and Doreen M. Floss -- 30. Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells / Thomas Schirrmann and Konrad Büssow -- Part IV. Recombinant Antibody Molecules in Nanobiotechnology and Proteomics -- 31. Immunoliposomes / Sylvia K.E. Messerschmidt, Julia Beuttler, and Miriam Rothdiener -- 32. Targeted Polymeric Nanoparticles / Katharina Landfester and Anna Musyanovych -- 33. Antibody Microarrays for Expression Analysis / Christoph Schröder, Anette Jacob, Sven Rüffer, Kurt Fellenberg, and Jörg D. Hoheisel -- 34. Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique / Zoltán Konthur and Jeannine Wilde -- Part V. Preclinical and Clinical Development -- 35. Xenograft Mouse Models for Tumour Targeting / Colin Green, Hakim Djeha, Gail Rowlinson-Busza, Christina Kousparou, and Agamemnon A. Epenetos -- 36. Xenograft Mouse Models for Tumour Targeting / Surinder K. Sharma and R. Barbara Pedley -- 37. Imaging Tumor Xenografts Using Radiolabeled Antibodies / Tove Olafsen, Vania E. Kenanova, and Anna M. Wu -- 38. Human Anti-antibody Response / Natalie L. Griffin, Hassan Shahbakhti, and Surinder K. Sharma -- 39. IP Issues in the Therapeutic Antibody Industry / Ulrich Storz and Alan J. Morrison -- Appendix: Amino Acids and Codons.
Summary: Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. ""Antibody Engineering"" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest 'hands on' knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include help
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Item type Current library Collection Call number Status Date due Barcode Item holds
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Total holds: 0

Previous edition: published in one volume. 2001.

Includes bibliographical references and index.

Part I. Bioinformatics of Antigen-binding Sites -- 1. Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool / Svetlana Mollova, Ida Retter, Michael Hust, Stefan Dübel, and Werner Müller -- 2. Standardized Sequence and Structure Analysis of Antibody Using IMGT1 / François Ehrenmann, Patrice Duroux, Véronique Giudicelli, and Marie-Paule Lefranc -- 3. Protein Sequence and Structure Analysis of Antibody Variable Domains / Andrew C.R. Martin -- Part II. Generation of Antibody Fragments and Their Derivatives -- 4. scFv by Two-Step Cloning / Dafne Müller -- 5. Bivalent Diabodies / Roland E. Kontermann -- 6. Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies) / Tove Olafsen, Vania E. Kenanova, and Anna M. Wu -- 7. Miniantibodies / Jonas V. Schaefer, Peter Lindner, and Andreas Plückthun -- 8. Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins / Athanasios Mavratzas, Evelyn Exner, Jürgen Krauss, and Michaela A.E. Arndt -- 9. Antibody-Cytokine Fusion Proteins with Members of the TNF-Family / Dafne Müller and Jeannette Gerspach -- 10. Recombinant Immunotoxins for Treating Cancer / Ira Pastan and Mitchell Ho -- 11. T Bodies / Bianca Altvater, Silke Landmeier, and Claudia Rossig -- 12. Expressing Intrabodies in Mammalian Cells / Alessio Cardinale and Silvia Biocca -- 13. Phenotypic Knockdown with Intrabodies / Nina Strebe and Manuela Schüngel -- 14. Disulfide-Stabilized Fv Fragments / Ulrich Brinkmann -- 15. PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics / Arutselvan Natarajan and Sally J. DeNardo -- 16. Fusion Proteins with Improved PK / Roland Stork -- 17. In Vivo Biotinylated scFv Fragments / Laila Al-Halabi and Torsten Meyer -- 18. Bispecific Diabodies and Single-Chain Diabodies / Roland E. Kontermann -- 19. Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-IgTM) Molecule / Chengbin Wu, Tariq Ghayur, and Jochen Salfeld -- 20. Isolation of Antigen-Specific Nanobodies / Gholamreza Hassanzadeh Ghassabeh, Dirk Saerens, and Serge Muyldermans -- 21. CDR-FR Peptides / Xiao-Qing Qiu -- Part III. Production of Antibody Fragments -- 22. Purification and Characterization of His-Tagged Antibody Fragments / Martin Schlapschy, Markus Fiedler, and Arne Skerra -- 23. Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium / Miriam Steinwand, Eva Jordan, and Michael Hust -- 24. Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L / Remko Griep and John McDougall -- 25. Purification and Analysis of Strep-tagged Antibody-Fragments / Martin Schlapschy and Arne Skerra -- 26. Production of Antibodies and Antibody Fragments in Escherichia coli / Dorothea E. Reilly and Daniel G. Yansura -- 27. Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones / Jonas V. Schaefer and Andreas Plückthun -- 28. Bioreactor Production of scFv Fragments in Pichia pastoris / Stephan Hellwig and Georg Melmer -- 29. Expression of Antibody Fragments in Transgenic Plants / Udo Conrad and Doreen M. Floss -- 30. Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells / Thomas Schirrmann and Konrad Büssow -- Part IV. Recombinant Antibody Molecules in Nanobiotechnology and Proteomics -- 31. Immunoliposomes / Sylvia K.E. Messerschmidt, Julia Beuttler, and Miriam Rothdiener -- 32. Targeted Polymeric Nanoparticles / Katharina Landfester and Anna Musyanovych -- 33. Antibody Microarrays for Expression Analysis / Christoph Schröder, Anette Jacob, Sven Rüffer, Kurt Fellenberg, and Jörg D. Hoheisel -- 34. Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique / Zoltán Konthur and Jeannine Wilde -- Part V. Preclinical and Clinical Development -- 35. Xenograft Mouse Models for Tumour Targeting / Colin Green, Hakim Djeha, Gail Rowlinson-Busza, Christina Kousparou, and Agamemnon A. Epenetos -- 36. Xenograft Mouse Models for Tumour Targeting / Surinder K. Sharma and R. Barbara Pedley -- 37. Imaging Tumor Xenografts Using Radiolabeled Antibodies / Tove Olafsen, Vania E. Kenanova, and Anna M. Wu -- 38. Human Anti-antibody Response / Natalie L. Griffin, Hassan Shahbakhti, and Surinder K. Sharma -- 39. IP Issues in the Therapeutic Antibody Industry / Ulrich Storz and Alan J. Morrison -- Appendix: Amino Acids and Codons.

Print version record.

Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. ""Antibody Engineering"" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest 'hands on' knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include help

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