Plant kinases : methods and protocols / edited by Nico Dissmeyer, Arp Schnittger.

Includes bibliographical references and index.

Guide to the book plant kinases / Nico Dissmeyer and Arp Schnittger -- Age of protein kinases / Nico Dissmeyer and Arp Schnittger -- Expression and purification of active protein kinases from wheat germ extracts / Boglarka Sonkoly, Viola Bardoczy, and Tamas Meszaros -- Measurement of plant cyclin-dependent kinase activity using immunoprecipitation-coupled and affinity purification-based kinase assays and the baculovirus expression system / Hirofumi Harashima and Masami Sekine -- Mitogen-activated protein kinase activity and reporter gene assays in plants / Robert Doczi, Elizabeth Hatzimasoura, and Laszlo Bogre -- Use of phospho-site substitutions to analyze the biological relevance of phosphorylation events in regulatory networks / Nico Dissmeyer and Arp Schnittger -- Bacterial assay to study plant sensor histidine kinases / Lukas Spichal -- Substrate analysis of Arabidopsis PP2C-type protein phosphatases / Julija Umbrasaite, Alois Schweighofer, and Irute Meskiene -- Modulating and monitoring MAPK activity during programmed cell death in pollen / Shutian Li and Vernonica E. Franklin-Tong -- Sensitizing plant protein kinases to specific inhibition by ATP-competitive molecules / Dor Salomon [and others] -- Fluorescence fluctuation analysis of receptor kinase dimerization / Mark A. Hink, Sacco C. de Vries, and Antonie J.W.G. Visser -- Quantifying degradation rates of transmembrane receptor kinases / Niko Geldner -- Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility In Planta / Mark Kwaaitaal [and others] -- Bimolecular-fluorescence complementation assay to monitor kinase-substrate interactions in vivo / Stefan Pusch, Nico Dissmeyer, and Arp Schnittger -- Chemical genetic analysis of protein kinase function in plants / Maik Bohmer, Michael Bolker, and Tina Romeis -- Modified metal-oxide affinity enrichment combined with 2D-PAGE and analysis of phosphoproteomes / Thomas Colby [and others] -- Phosphoproteomics using iTRAQ / Alexandra M.E. Jones and Thomas S. Nuhse.

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Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.