Microbial carotenoids from fungi : methods and protocols / edited by José-Luis Barredo.

Includes bibliographical references and index.

Print version record.

Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage, and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids From Fungi: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the manipulation and metabolic engineering of the Beta-carotene producing fungi Blakeslea trispora and Mucor circinelloides, lycopene production with the yeast Yarrowia lipolytica, peroxisome targeting of lycopene pathway enzymes in Pichia pastoris, and the manipulation of the heterobasidiomycetous yeast Xanthophyllomyces dendrorhous, which produces astaxanthin, a red xanthophyll with large importance in the aquaculture, pharmaceutical, and food industries. Additionally, the book includes a DNA assembler method for construction of zeaxanthin-producing strains of Saccharomyces cerevisiae, production of neurosporaxanthin by Neurospora and Fusarium, and production of torularhodin, torulene and b-carotene by Rhodotorula yeasts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Carotenoids From Fungi: Methods and Protocols, provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms.

English.

Essential role of genetics in the advancement of biotechnology / Arnold L. Demain and José L. Adrio -- Microbial carotenoids / Preejith Vachali, Prakash Bhosale, and Paul S. Bernstein -- Biosynthesis, extraction, purification, and analysis of trisporoid sexual communication compounds from mated cultures of Blakeslea trispora / C. Schimek and J. Wöstemeyer -- Isolation of mutants and construction of intersexual heterokaryons of Blakeslea trispora / Enrique Cerdá-Olmedo and Bina J. Mehta -- Molecular tools for carotenogenesis analysis in the zygomycete Mucor circinelloides / Santiago Torres-Martínez, Rosa M. Ruiz-Vázquez, Victoriano Garre, Sergio López-García, Eusebio Navarro, Ana Vila -- Gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides / Enrique A. Iturriaga, Tamás Papp, María Isabel Álvarez, Arturo P. Eslava -- Integration of a bacterial Ý-carotene ketolase gene into the Mucor circinelloides genome by the Agrobacterium tumefaciens-mediated transformation method / Tamás Papp, Árpád Csernetics, Ildikó Nyilasi, Csaba Vágvölgyi, Enrique A. Iturriaga -- Metabolic engineering of Mucor circinelloides for zeaxanthin production / Marta Rodríguez-Sáiz, Juan-Luis de la Fuente, José-Luis Barredo -- Bioengineering of oleaginous yeast Yarrowia lipolytica for lycopene production / Rick W. Ye, Pamela L. Sharpe, Quinn Zhu -- Peroxisome targeting of lycopene pathway enzymes in Pichia pastoris / Pyung Cheon Lee -- Production, extraction, and quantification of astaxanthin by Xanthophyllomyces dendrorhous or Haematococcus pluvialis : standardized techniques / Alma Rosa Domínguez-Bocanegra -- Isolation and selection of new astaxanthin producing strains of Xanthophyllomyces dendrorhous / Diego Libkind, Martín Moliné, Celia Tognetti -- Isolation and characterization of extrachromosomal double-stranded RNA elements in Xanthophyllomyces dendrorhous / Marcelo Baeza, María Fernández-Lobato, Víctor Cifuentes -- Isolation of carotenoid hyperproducing mutants of Xanthophyllomyces dendrorhous (Phaffia rhodozyma) by flow cytometry and cell sorting / Byron F. Brehm-Stecher, Eric A. Johnson -- Generation of astaxanthin mutants in Xanthophyllomyces dendrorhous using a double recombination method based on hygromycin resistance / Mauricio Niklitschek, Marcelo Baeza, María Fernández-Lobato, Víctor Cifuentes -- Genetic manipulation of Xanthophyllomyces dendrorhous and Phaffia rhodozyma / Guangyun Lin, Joanna Bultman, Eric A. Johnson, Jack W. Fell -- DNA assembler method for construction of zeaxanthin-producing strains of Saccharomyces cerevisiae / Zengyi Shao, Yunzi Luo, Huimin Zhao -- Neurosporaxanthin production by Neurospora and Fusarium / Javier Avalos, Alfonso Prado-Cabrero, Alejandro F. Estrada -- Production of torularhodin, torulene, and Ý-carotene by Rhodotorula Yeasts / Martín Moliné, Diego Libkind, María van Broock.