Molecular dermatology : methods and protocols / edited by Cristina Has and Cassian Sitaru.

Includes bibliographical references and index.

Molecular dermatology comes of age / Cristina Has and Cassian Sitaru -- Molecular diagnosis of autoimmune blistering diseases / Daisuke Tsuruta [and others] -- Molecular diagnosis of genodermatoses / Vesarat Wessagowit -- Three-dimensional visualization of the molecular architecture of cell-cell junctions in situ by cryo-electron tomography of vitreous sections / Ashraf Al-Amoudi and Achilleas S. Frangakis -- 3D visualization of epidermal Langerhans cells / Akiharu Kubo, Keisuke Nagao, and Masayuki Amagai -- In vivo imaging of lymph node lymphangiogenesis by immuno-positron emission tomography / Viviane Mumprecht and Michael Detmar -- Optical imaging of HPV infection in a murine model / Rhonda C. Kines [and others] -- Laser scanning microscopy approach for semiquantitation of in vitro dermal particle penetration / Hagar I. Labouta, Ulrich F. Schaefer, and Marc Schneider -- Analysis of cutaneous somatic mosaicism / Anna M.G. Pasmooij and Marcel F. Jonkman -- Global proteome analyses of SILAC-gabeled skin cells / Adrian Sprenger [and others] -- MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR method / Janosch Hildebrand [and others] -- Cell death in the skin : how to study its quality and quantity? / Roman Makarov [and others] -- In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus / Ken Ishii and Masayuki Amagai -- Induction of granulocyte-dependent dermal-epidermal separation by autoantibodies ex vivo / Mircea Teodor Chiriac -- Analysis of collective invasion of carcinoma cells in a 3D organotypic model / Jean Albrengues, Gerrino Meneguzzi, and Cedric Gaggioli -- Isolation of melanoma tumor-initiating cells from surgical tissues / Alexander D. Boiko -- Detection, enumeration, and characterization of immune cells infiltrating melanoma tumors / Benjamin Toh [and others] -- Analysis of cell movement between skin and other anatomical sites in vivo using photoconvertible fluorescent protein "Kaede"-transgenic mice / Michio Tomura and Kenji Kabashima -- Reconstitution of skin fibrosis development using a tissue engineering approach / Véronique J. Moulin -- Bioengineered skin humanized model of psoriasis / Marta Carretero, Sara Guerrero-Aspizua, and Marcela Del Río -- Induction of contact hypersensitivity in the mouse model / Stefan F. Martin --Generation of functional multipotent keratinocytes from mouse induced and pluripotent stem cells / Ganna Bilousova and Dennis R. Roop -- RNAi-mediated gene function analysis in skin / Slobodan Beronja and Elaine Fuchs -- Mouse models of autoimmune blistering diseases induced by the passive transfer of antibodies / Wataru Nishie -- induction of experimental epidermolysis bullosa acquisita by immunization with murine collagen VII / Alina Sesarman and Cassian Sitaru -- Animal model for cutaneous leishmaniasis / Michael R. Fischer [and others] -- Model for generation of large numbers of primary inflammatory skin-derived neutrophils and macrophages / Dominik John, Michael R. Fischer, and Esther von Stebut -- Zebrafish as a model system to study heritable skin diseases / Qiaoli Li and Jouni Uitto -- Cell- and protein-based therapy approaches for epidermolysis bullosa / Alexander Nyström, Leena Bruckner-Tuderman, and Johannes S. Kern -- RNA trans-splicing for genodermatoses / Johann W. Bauer [and others].

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases. In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology. As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.

English.