Plant cell morphogenesis : methods and protocols / edited by Viktor Žárský, Fatima Cvrčková.

Includes bibliographical references and index.

Essential Methods of Plant Sample Preparation for Light Microscopy / Aleš Soukup and Edita Tylová -- Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy / Aleš Soukup -- Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues / Kieran J.D. Lee and J. Paul Knox -- Automated Microscopy in Forward Genetic Screening of Arabidopsis / Tereza Dobisová and Jan Hejátko -- Image Analysis: Basic Procedures for Description of Plant Structures / Jana Albrechtová, Zuzana Kubínová, Aleš Soukup, and Jiří Janáček -- Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells / Andreas Nebenführ -- Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM / Amparo Rosero, Viktor Žárský, and Fatima Cvrčková -- Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces / Dorota Kwiatkowska and Agata Burian -- Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope / Olivier Hamant, Pradeep Das, and Agata Burian -- Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX / Pierre Barbier de Reuille, Sarah Robinson, and Richard S. Smith -- Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy / Anne-Lise Routier-Kierzkowska and Richard S. Smith -- High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy / Ichirou Karahara and Byung-Ho Kang -- Reconstructing Plant Cells in 3D by Serial Section Electron Tomography Kiminori Toyooka and Byung-Ho Kang -- Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy / Jindřiška Fišerová and Martin W. Goldberg -- Immunogold Labeling of Resin-embedded Electron Microscopical Sections / Ilse Foissner and Margit Hoeftberger -- Live Cell Imaging of Arabidopsis Root Hairs / Tijs Ketelaar -- Morphological Analysis of Cell Growth Mutants in Physcomitrella / Jeffrey P. Bibeau and Luis Vidali -- Plant Cell Lines in Cell Morphogenesis Research / Daniela Seifertová, Petr Klíma, Markéta Pařezová, Jan Petrášek, Eva Zažímalová, and Zdeněk Opatrný -- Antisense Oligodeoxynucleotide-mediated Gene Knockdown in Pollen Tubes / Radek Bezvoda, Roman Pleskot, Viktor Žárský, and Martin Potocký-- Lab-on-a-Chip for Studying Growing Pollen Tubes / Carlos G. Agudelo, Muthukumaran Packirisamy, and Anja Geitmann -- Laser Microdissection of Plant Cells / Yvonne Ludwig and Frank Hochholdinger -- Optical Trapping in Plant Cells / Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren -- Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery / Fatima Cvrčková and Michal Hála.

Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on classical light microscopy and histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized electron microscopy techniques, and methods used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by expert researchers in the field and include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Print version record and online resource; title from PDF title page (Springer Protocols, viewed January 29, 2014).

English.