Small non-coding RNAs : methods and protocols / edited by Mathieu Rederstorff.

Includes bibliographical references and index.

Small non-coding RNAs : a quick look in the rearview mirror / Guillaume Clerget, Yoann Abel, and Mathieu Rederstorff -- Alcoholic precipitation of small non-coding RNAs / Guillaume Clerget, Valérie Bourguignon-Igel, and Mathieu Rederstorff -- Quantification and quality control of a small non-coding RNA preparation / Virginie Marchand and Christiane Branlant -- Impact of RNA isolation protocols on RNA detection by northern blotting / Katrin Damm [and others] -- Improved northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes / Katrin Damm [and others] -- Direct cloning of double-stranded RNAs / Manli Shen [and others] -- Detection and labeling of small non-coding RNAs by splinted ligation / Gabrielle Bourgeois [and others] -- Fluorescence in situ hybridization of small non-coding RNAs / Valentin Vautrot [and others] -- RT-qPCR-based quantification of Small Non-Coding RNAs / Fjoralba Zeka, Pieter Mestdagh, and Jo Vandesompele -- Stem-Loop RT-PCR based quantification of small non-coding RNAs / Véronique Salone and Mathieu Rederstorff -- miR-RACE : an effective approach to accurately determine the sequence of computationally identified miRNAs / Chen Wang and Jinggui Fang -- Probing small non-coding RNAs structures / Jean-Vincent Philippe [and others] -- cDNA library generation for the analysis of small RNAs by high-throughput sequencing / Jennifer Gebetsberger, Roger Fricker, and Norbert Polacek -- CLIP-Seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells / Jérôme Saulière and Hervé Le Hir -- Microarray analysis of small non-coding RNAs / Michael Karbiener and Marcel Scheideler -- RML-RACE, PPM-RACE, and qRT-PCR : an integrated strategy to accurately validate miRNA target genes / Chen Wang and Jinggui Fang -- Dual luciferase gene reporter assays to study miRNA function / Thomas Clément, Véronique Salone, and Mathieu Rederstorff -- Gene expression knockdown by transfection of siRNAs into mammalian cells / Yoann Abeland Mathieu Rederstorff -- Efficient and selective knockdown of small non-coding RNAs / Xue-Hai Liang, Wen Shen, and Stanley T. Crooke -- Cell-SELEX : in vitro selection of synthetic small specific ligands / Helena Dickinson [and others] -- Small non-coding RNAs and aptamers in diagnostics and therapeutics / Marissa Leonard, Yijuan Zhang, and Xiaoting Zhang.

Online resource; title from PDF title page (SpringerLink, viewed March 27, 2015).

This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular, and molecular biologists already working in the field of RNA biology and to those just starting to study small non-coding RNAs.