Plant mitochondria : methods and protocols / edited by James Whelan, Monika W. Murcha.

Includes bibliographical references and index.

Isolation of intact mitochondria from the model plant species Arabidopsis thaliana and Oryza sativa / Monika W. Murcha and James Whelan -- Determining mitochondrial transcript termini for the study of transcription start sites and transcript 5' end maturation / Stefan Binder and Kristina Kühn -- Mitochondrial run-on transcription assay using biotin labeling / Kristina Kühn -- In vitro RNA uptake studies in plant mitochondria / Szymon Kubiszewski-Jakubiak [and five others] -- In vitro and in vivo protein uptake studies in plant mitochondria / Owen Duncan [and three others] -- Plant mitochondrial proteomics / Nicolas L. Taylor and A. Harvey Millar -- Identification of lysine-acetylated mitochondrial proteins and their acetylation sites / Markus Hartl, Ann-Christine König, and Iris Finkemeier -- Flowchart to analyze protease activity in plant mitochondria / Pedro F. Teixeira [and three others] -- Activity measurements of mitochondrial enzymes in native gels / Peter Schertl and Hans-Peter Braun -- Activity assay for plant mitochondrial enzymes / Shaobai Huang, Chun Pong Lee, and A. Harvey Millar -- Analysis of type II NAD(P)H dehydrogenases / Kathleen L. Soole and Chevaun A. Smith -- Assessment of respiration in isolated plant mitochondria using clark-type electrodes / Richard P. Jacoby, A. Harvey Millar, and Nicolas L. Taylor -- Micro-respiratory measurements in plants / Yun Shin Sew, A. Harvey Millar, and Elke Stroeher -- Improvements to define mitochondrial metabolomics using nonaqueous fractionation / Richard Fly [and four others] -- Mitochondrial markers of programmed cell death in Arabidopsis thaliana / Theresa J. Reape [and others] -- Imaging and analysis of mitochondrial dynamics in living cells / Sanjaya B. Ekanayake [and four others] -- Analysis of plant mitochondrial function using fluorescent protein sensors / Stephan Wagner [and six others] -- In planta analysis of leaf mitochondrial superoxide and nitric oxide / Marina Cvetkovska and Greg C. Vanlerberghe -- Databases and informatics resources for analysis of plant mitochondria / Reena Narsai -- Expression and crystallization of the plant alternative oxidase / Benjamin May [and six others].

Online resource; title from PDF title page (SpringerLink, viewed April 29, 2015).

Annotation The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.