Methods in mouse atherosclerosis / edited by Vicente Andrés, Beatriz Dorado.

Includes bibliographical references and index.

Use of mouse models in atherosclerosis research / Godfrey S. Getz and Catherine A. Reardon -- Cytokines and immune responses in murine atherosclerosis / Pascal J.H. Kusters and Esther Lutgens -- Intravital microscopy for atherosclerosis research / Remco T.A. Megens and Oliver Soehnlein -- Noninvasive molecular imaging of mouse atherosclerosis / Martina A. McAteer and Robin P. Choudhury -- Oil red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root / M. Jesús Andrés-Manzano, Vicente Andrés, and Beatriz Dorado -- Isolation, culture, and polarization of murine bone marrow-derived and peritoneal macrophages / Inés Pineda-Torra [and others] -- Isolation of mouse primary aortic endothelial cells by selection with specific antibodies / Pedro Molina-Sánchez and Vicente Andrés -- Isolation and culture of aortic smooth muscle cells and in vitro calcification assay / Ricardo Villa-Bellosta and Magda R. Hamczyk -- Immunostaining of macrophages, endothelial cells, and smooth muscle cells in the atherosclerotic mouse aorta / Prashanthi Menon and Edward A. Fisher -- Immunostaining of lymphocytes in mouse atherosclerotic plaque / Anton Gisterå and Daniel F.J. Ketelhuth -- Flow cytometric analysis of immune cells within murine aorta / Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, and Elena V. Galkina -- In vitro differentiation of naïve CD4+ T cells : a tool for understanding the development of atherosclerosis / Salvador Iborra and Jose M. González-Granado -- Quantification of apoptosis in mouse atherosclerotic lesions / Nichola L. Figg and Martin R. Bennett -- Quantification of cellular proliferation in mouse atherosclerotic lesions / José J. Fuster -- Quantification of in vitro macrophage cholesterol efflux and in vivo macrophage-specific reverse cholesterol transport / Joan Carles Escolà-Gil [and others] -- In vitro macrophage phagocytosis assay / Magda R. Hamczyk, Ricardo Villa-Bellosta, and Vicente Andrés -- Glucose and insulin tolerance tests in the mouse / Ángela Vinué and Herminia González-Navarro -- Wire myography to study vascular tone and vascular structure of isolated mouse arteries / Lara del Campo and Mercedes Ferrer -- Pressure myography to study the function and structure of isolated small arteries / Olav L. Schjørring, Rune Carlsson, and Ulf Simonsen -- Generation of aorta transcript atlases of wild-type and apolipoprotein E-null mice by laser capture microdissection-rased mRNA expression microarrays / Changjun Yin [and others] -- Analysis of gene and protein expression in atherosclerotic mouse aorta by western blot and quantitative real-time PCR / José Rivera-Torres -- Bone marrow transplantation in mice to study the role of hematopoietic cells in atherosclerosis / Vinatha Sreeramkumar and Andrés Hidalgo -- Tandem stenosis to induce atherosclerotic plaque instability in the mouse / Yung Chih Chen, Jennifer Rivera, and Karlheinz Peter -- Detection of intraplaque hemorrhage in mouse atherosclerotic lesions / Judith C. Sluimer, Marion J. Gijbels, and Sylvia Heeneman -- Mechanical stabilization of mouse carotid artery for in vivo intravital microscopy imaging of atherogenesis / Raphaël Chèvre -- Intravital microscopy in the cremaster muscle microcirculation for endothelial dysfunction studies / Cristina Rius and María J. Sanz -- Fluorescent molecular tomography for in vivo imaging of mouse atherosclerosis / Alicia Arranz [and others] -- In vivo 18F-FDG-PET imaging in mouse atherosclerosis / Jesús Mateo [and others] -- Magnetic resonance imaging of the atherosclerotic mouse aorta / Jesús Mateo [and others].

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors' personal wet lab experience.