Plant cell division : methods and protocols / edited by Marie-Cecile Caillaud.

Includes bibliographical references and index.

Synchronization of green algae by light and dark regimes for cell cycle and cell division studies / Monika Hlavová, Milada Vítová, and Kateřina Bišová -- Plant cell division analyzed by transient Agrobacterium-Mediated transformation of tobacco BY-2 cells / Henrik Buschmann -- Identifying gene regulatory networks in arabidopsis by in silico prediction, yeast-1-hybrid, and inducible gene profiling assays / Erin E. Sparks and Philip N. Benfey -- Cytrap marker systems for in vivo visualization of cell cycle progression in Arabidopsis / Shiori Sugamata Aki and Masaaki Umeda -- Imaging nuclear morphology and organization in cleared plant tissues treated with cell cycle inhibitors / José Dijair Antonino de Souza Junior [and others] -- Isolation of plant nuclei at defined cell cycle stages using EdU labeling and flow cytometry / Emily E. Wear [and others] -- Preparation and fluorescent analysis of plant metaphase chromosomes / Trude Schwarzacher -- Fluorescence in situ hybridization on rice chromosomes / Yafei Li and Zhukuan Cheng -- Dynamics of the plant nuclear envelope during cell division / David E. Evans and Katja Graumann -- Immunolabeling of nuclei/chromosomes in Arabidopsis thaliana / Michael Sandmann, Joerg Fuchs, and Inna Lermontova -- In vivo imaging of microtubule organization in dividing giant cell / Marie-Cécile Caillaud and Bruno Favery -- Real-time lineage analysis to study cell division orientation in the arabidopsis shoot meristem / Cory J. Tobin and Elliot M. Meyerowitz -- Cell proliferation analysis using EdU labeling in whole plant and histological samples of arabidopsis / Anita Kazda [and others] -- Studying cell division plane positioning in early-stage embryos / Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia -- Characterization of cytokinetic mutants using small fluorescent probes / Andrei Smertenko [and others] -- Using live-cell markers in maize to analyze cell division orientation and timing / Carolyn G. Rasmussen -- Ratiometric fluorescence live imaging analysis of membrane lipid order in arabidopsis mitotic cells using a lipid order-sensitive probe / Patricia Gerbeau-Pissot [and others].

This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.