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Hydrocarbon and lipid microbiology protocols : biochemical methods / Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors.

Contributor(s): Material type: TextTextSeries: Springer protocols (Series)Publisher: Berlin : Springer, 2016Description: 1 online resource (x, 188 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9783662491379
  • 3662491370
Subject(s): Genre/Form: Additional physical formats: Print version:: Hydrocarbon and lipid microbiology protocols.DDC classification:
  • 572/.57 23
LOC classification:
  • QP751
NLM classification:
  • 2016 I-103
  • QD 305.H5
Online resources:
Contents:
Introduction -- Protocols for the isolation and analysis of lipopeptides and bioemulsifiers -- Protocols for the detection and chemical characterisation of microbial glycolipids -- Isolation of glycoprotein bioemulsifiers produced by marine bacteria -- Protocols for lipid extraction from wet algal biomass -- Isolation and characterization of lipid droplets from yeast -- Protocols for Isolation and Analysis of Polyhydroxyalkanoates -- Purification of lipid rafts from bacterial membranes -- Isolation and analysis of membrane phospholipids as biomarkers -- Protocols for purifying and characterizing integral membrane AlkB enzymes -- Protocols for structural and functional analysis of particulate methane monooxygenase from Methylocystis species strain Rockwell (ATCC 49242) -- Phenotype-based Identification of key enzymes for polycyclic aromatic hydrocarbon (PAH) metabolism from Mycobacteria using transposon mutagenesis and a PAH spray plate -- Microcalorimetry as a general technique to characterize ligand binding.
Summary: This volume provides protocols for the biochemical analysis of hydrocarbon - and lipid - relevant products, cell components and activities of microbes that interact with hydrophobic compounds. It includes methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates.
Holdings
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Total holds: 0

Online resource; title from PDF title page (SpringerLink, viewed September 1, 2016).

This volume provides protocols for the biochemical analysis of hydrocarbon - and lipid - relevant products, cell components and activities of microbes that interact with hydrophobic compounds. It includes methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates.

Introduction -- Protocols for the isolation and analysis of lipopeptides and bioemulsifiers -- Protocols for the detection and chemical characterisation of microbial glycolipids -- Isolation of glycoprotein bioemulsifiers produced by marine bacteria -- Protocols for lipid extraction from wet algal biomass -- Isolation and characterization of lipid droplets from yeast -- Protocols for Isolation and Analysis of Polyhydroxyalkanoates -- Purification of lipid rafts from bacterial membranes -- Isolation and analysis of membrane phospholipids as biomarkers -- Protocols for purifying and characterizing integral membrane AlkB enzymes -- Protocols for structural and functional analysis of particulate methane monooxygenase from Methylocystis species strain Rockwell (ATCC 49242) -- Phenotype-based Identification of key enzymes for polycyclic aromatic hydrocarbon (PAH) metabolism from Mycobacteria using transposon mutagenesis and a PAH spray plate -- Microcalorimetry as a general technique to characterize ligand binding.

Includes bibliographical references.

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