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T-cell motility : methods and protocols / edited by Navin Kumar Verma.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1930.Publisher: New York : Humana Press : Springer, [2019]Description: 1 online resource (x, 158 pages) : illustrations (chiefly color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781493990368
  • 1493990365
Subject(s): Genre/Form: Additional physical formats: Print version:: T-cell motility.DDC classification:
  • 571.9/66 23
LOC classification:
  • QR185.8.T2
NLM classification:
  • W1
  • QW 25
Online resources:
Contents:
Introduction to LFA-1/ICAM-1 interactions in T-cell motility / Navin Kumar Verma and Dermot Kelleher -- Isolation of human peripheral blood T-lymphocytes / Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma -- Laboratory model to study T-cell motility / Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher -- Quantification of T-cell migratory phenotypes using high-content analysis / Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma -- Live cell imaging and analysis to capture T-cell motility in real-time / Seow Theng Ong and Navin Kumar Verma -- Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells / Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma -- Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis / Praseetha Prasannan and Navin Kumar Verma -- Quantitative real-time PCR for Evaluating Transcriptional Changes in T-Lymphocytes / Atish Kizhakeyil and Navin Kumar Verma -- GapmeR-mediated gene silencing in motile T-cells / Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, and Navin Kumar Verma -- Live imaging of resident T-cell migration in human lymphoid tissue slices using confocal microscopy / Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann -- Phosphoprotein enrichment for protein analysis in motile T-lymphocytes / Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma -- Immunometabolomic phenotyping of motile T-cells / Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma -- Profiling activity of cellular kinases in migrating T-cells / Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and WimVanden Berghe -- Biotinylation assay to determine LFA-1 recycling in motile T-lymphocytes / Malin Samuelsson and Lena M. Svensson -- Protocol to study T-cell signaling in an immune synapse by microscopy / Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, and Navin Kumar Verma -- Enzyme-linked immunosorbent assay for T-cell dependent immunogenicity assessment of therapeutic peptides / Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma -- Modified intravital microscopy to assess vascular health and T-cell motility / Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers -- Computational analysis of protein-protein interactions in motile T-cells / Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma.
Summary: This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging.
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Online resource; title from PDF title page (SpringerLink, viewed January 7, 2019).

Includes bibliographical references and index.

Introduction to LFA-1/ICAM-1 interactions in T-cell motility / Navin Kumar Verma and Dermot Kelleher -- Isolation of human peripheral blood T-lymphocytes / Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma -- Laboratory model to study T-cell motility / Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher -- Quantification of T-cell migratory phenotypes using high-content analysis / Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma -- Live cell imaging and analysis to capture T-cell motility in real-time / Seow Theng Ong and Navin Kumar Verma -- Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells / Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma -- Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis / Praseetha Prasannan and Navin Kumar Verma -- Quantitative real-time PCR for Evaluating Transcriptional Changes in T-Lymphocytes / Atish Kizhakeyil and Navin Kumar Verma -- GapmeR-mediated gene silencing in motile T-cells / Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, and Navin Kumar Verma -- Live imaging of resident T-cell migration in human lymphoid tissue slices using confocal microscopy / Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann -- Phosphoprotein enrichment for protein analysis in motile T-lymphocytes / Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma -- Immunometabolomic phenotyping of motile T-cells / Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma -- Profiling activity of cellular kinases in migrating T-cells / Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and WimVanden Berghe -- Biotinylation assay to determine LFA-1 recycling in motile T-lymphocytes / Malin Samuelsson and Lena M. Svensson -- Protocol to study T-cell signaling in an immune synapse by microscopy / Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, and Navin Kumar Verma -- Enzyme-linked immunosorbent assay for T-cell dependent immunogenicity assessment of therapeutic peptides / Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma -- Modified intravital microscopy to assess vascular health and T-cell motility / Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers -- Computational analysis of protein-protein interactions in motile T-cells / Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma.

This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging.

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