Nanotechnology for nucleic acid delivery : methods and protocols / edited by Manfred Ogris, Haider Sami.

Includes bibliographical references and index.

Sequence-defined cationic lipo-oligomers containing unsaturated fatty acids for transfection / Soren Reinhard and Ernst Wagner -- Synthesis of Bioreducible polycations with controlled topologies / Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, and David Oupicky -- Histidine-rich cationic cell-penetrating peptides for plasmid DNA and siRNA Delivery / Antoine Kichler, A. James Mason, Arnaud Marquette, and Burkhard Bechinger -- Covalent fluorophore labeling of oligonucleotides and generation of other oligonucleotide bioconjugates / Cornelia Lorenzer and Johannes Winkler -- Synthetic oligonucleotides in SPECT/CT In vivo imaging : chemical modifications, In111 complex formation, incorporation into drug delivery systems / Maxim Antopolsky -- Synthesis of polyethylenimine-based nanocarriers for systemic tumor targeting of nucleic acids / Wolfgang Rodl, Alexander Taschauer, David Schaffert, and Ernst Wagner, and Manfred Ogris -- Cationic photopolymerized polydiacetylenic (PDA) micelles for siRNA delivery / Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, and Antoine Kichler -- Lipids for nucleic acid delivery : cationic or neutral lipoplexes, synthesis, and particle formation / Michel Bessodes, Helene Dhotel, and Nathalie Mignet -- Preparation, characterization, and in vitro evaluation of lipidoid-polymer hybrid nanoparticles for siRNA delivery to the cytosol / Kaushik Thanki, Xianghui Zeng, and Camilla Foged -- Layer-by-layer assembled nanoparticles for siRNA delivery / Michaela Guter and Miriam Breunig -- Layer-by-layer film engineering for sequential gene delivery / Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, and Guangzhao Mao -- Surface- and Hydrogel-mediated delivery of nucleic acid nanoparticles / Angela K. Pannier, Tyler Kozisek, and Tatiana Segura -- In situ AFM analysis investigating disassembly of DNA nanoparticles and nanofilms / Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, and Guangzhao Mao -- Lyophilization of synthetic gene carriers / Julia Christina Kasper, Sarah Hedtrich, and Wolfgang Friess -- Firefly luciferase-based reporter gene assay for investigating nanoparticle-mediated nucleic acid delivery / Katharina Muller, Manfred Ogris, and Haider Sami -- Enhancing nucleic acid delivery with ultrasound and microbubbles / Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, and Ine Lentacker -- Magnetic and acoustically active microbubbles loaded with nucleic acids for gene delivery / Dialechti Vlaskou, Olga Mykhaylyk, and Christian Plank -- Lactate dehydrogenase assay for assessment of polycation cytotoxicity / Ladan Parhamifar, Helene Andersen, and S. Moein Moghimi -- Combined fluorimetric caspase-3/7 assay and bradford protein determination for assessment of polycation-mediated cytotoxicity / Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, and S. Moein Moghimi -- Determination of polycation-mediated perturbation of mitochondrial respiration in intact cells by high-resolution respirometry (Oxygraph-2k, OROBOROS) / Arnaldur Hall and S. Moein Moghimi -- Evaluating the regulation of cytokine levels after siRNA treatment in antigen-specific target cell populations via intracellular staining / Rima Kandil, Daniel Feldmann, Yuran Xie, and Olivia M. Merkel -- Anti-PEG IgM production via a pegylated nanocarrier system for nucleic acid delivery / Amr S. Abu Lila and Tatsuhiro Ishida -- Near-infrared optical imaging of nucleic acid nanocarriers in vivo / Claire Rome, Julien Gravier, Marie Morille, Gilles Divita, Anne-Laure Bolcato-Bellemin, Veronique Josserand, and Jean-Luc Coll -- Flow cytometry-based cell type-specific assessment of target regulation by pulmonary siRNA delivery / Olivia M. Merkel, Leigh M. Marsh, Holger Garn, and Thomas Kissel -- Microbubbles for nucleic acid delivery in liver using mild sonoporation / Nathalie Mignet, Corinne Marie, Anthony Delalande, Simona Manta, Michel-Francis Bureau, Gilles Renault, Daniel Scherman, and Chantal Pichon -- Lipopeptide delivery of siRNA to the central nervous system / Mark D. Zabel, Luke Mollnow, and Heather Bender.

Online resource; title from PDF title page (SpringerLink, viewed March 6, 2019).

This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself.