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CryoEM : methods and protocols / edited by Tamir Gonen, Brent L. Nannenga.

Contributor(s): Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 2215.Publisher: New York, NY : Humana Press, [2021]Description: 1 online resource (xi, 350 pages) : illustrations (some color)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781071609668
  • 1071609661
  • 1071609653
  • 9781071609651
Subject(s): Additional physical formats: Printed edition: No title; Printed edition: No title; Printed edition: No titleDDC classification:
  • 502.8/25 23
LOC classification:
  • QH225
NLM classification:
  • W1 ME9616J
  • QY 95
Online resources:
Contents:
Getting Started with in situ cryo-electron Tomography -- Cryo-Electron Tomography and Automatic Segmentation of Cultured Hippocampal Neurons -- Practical Approaches for Cryo-FIB Milling and applications for Cellular Cryo-Electron Tomography -- In situ Imaging and Structure Determination of Biomolecular Complexes using Cryo-Electron Tomography -- Progress Towards CryoEM: Negative-stain Procedures for Biological Samples -- Setting up Parallel Illumination on the Talos Arctica for High-Resolution Data Collection -- Multi-body refinement of cryo-EM images in RELION -- Evaluating Local and Directional Resolution of Cryo-EM Density Maps -- Automated modeling and Validation of Protein Complexes in cryo-EM Maps -- 2D Electron Crystallography of Membrane Protein Single-, Double- and Multi-Layered Ordered Arrays -- Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-Protein Interaction -- Single Particle Analysis for High Resolution 2D Electron Crystallography -- MicroED Sample Preparation and Data Collection for Protein Crystals. -- Detection of Microcrystals for CryoEM -- Low-dose Data Collection and Radiation Damage in MicroED -- Automation of Continuous-rotation Data Collection for MicroED -- Ab initio Determination of Peptide Structures by MicroED.
Summary: This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology.
Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
eBook eBook e-Library eBook Protocols Available
Total holds: 0

Includes index.

This volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology.

Getting Started with in situ cryo-electron Tomography -- Cryo-Electron Tomography and Automatic Segmentation of Cultured Hippocampal Neurons -- Practical Approaches for Cryo-FIB Milling and applications for Cellular Cryo-Electron Tomography -- In situ Imaging and Structure Determination of Biomolecular Complexes using Cryo-Electron Tomography -- Progress Towards CryoEM: Negative-stain Procedures for Biological Samples -- Setting up Parallel Illumination on the Talos Arctica for High-Resolution Data Collection -- Multi-body refinement of cryo-EM images in RELION -- Evaluating Local and Directional Resolution of Cryo-EM Density Maps -- Automated modeling and Validation of Protein Complexes in cryo-EM Maps -- 2D Electron Crystallography of Membrane Protein Single-, Double- and Multi-Layered Ordered Arrays -- Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-Protein Interaction -- Single Particle Analysis for High Resolution 2D Electron Crystallography -- MicroED Sample Preparation and Data Collection for Protein Crystals. -- Detection of Microcrystals for CryoEM -- Low-dose Data Collection and Radiation Damage in MicroED -- Automation of Continuous-rotation Data Collection for MicroED -- Ab initio Determination of Peptide Structures by MicroED.

Online resource; title from PDF title page (SpringerLink, viewed March 3, 2021).

Includes bibliographical references and index.

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